Fabrication and Characterization of Silicon Based Vertical Electrode Nanogap Biosensor for Protein Detection PDF Download

Author: Norbi Hayati Mohd Noor
Publisher:
ISBN:
Category : Nanobiotechnology
Languages : en
Pages : 0

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Book Description
The main objective of this research is to design, fabricate, characterize, and test the silicon based vertical electrode Nanogap biosensor which will be used to detect and identify target proteins in aqueous solution.

Author: Norbi Hayati Mohd Noor
Publisher:
ISBN:
Category : Nanobiotechnology
Languages : en
Pages : 112

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Book Description
The main objective of this research is to design, fabricate, characterize, and test the silicon based vertical electrode Nanogap biosensor which will be used to detect and identify target proteins in aqueous solution.

Author: Norbi Hayati Bt Mohd Noor
Publisher:
ISBN:
Category :
Languages : en
Pages : 58

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Author: Thakra S. Dhahi
Publisher:
ISBN:
Category : Electrodes
Languages : en
Pages : 165

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Book Description
The aim of this research work is to design, fabricate, characterize, and test nanogap-electrode based device for biochemical detection and DNA immobilization and hybridization detection. However, the focus of this research is to investigate electrically and chemically the effect of different materials and gap sizes on the nanogap electrodes design.

Author: Nazwa Taib
Publisher:
ISBN:
Category : Nanoelectronics
Languages : en
Pages : 91

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Book Description
The aim of this research is to design, fabricate and characterize an array of planar nanogap capacitive sensor based on Poly-SiO2 structure by using size expansion technique.

Author: Bonhye Koo
Publisher:
ISBN:
Category :
Languages : en
Pages : 155

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Book Description
Pressing performance demands require next-generation biosensors to detect target chemical and biological molecules with higher sensitivity, shorter response times, and lower detection limit. Micro- and nanoscale devices are attractive for a wide range of biosensor applications since at small scale, in addition to being more compact, the device may exhibit improved performance. The benefits include minimization of tissue damage for implantable devices, improved spatial resolution and sensitivity, as well as increased surface charge to mass ratio, which is important for the performance of our novel technology for nucleic acid detection described below. Borrowing from the processing technologies used in the semiconductor industry, we implemented micromachining techniques to fabricate devices at both the micro- and nanoscale. In this dissertation, we present our work on the fabrication and characterization of two next-generation biosensors. The first device we fabricated is a sequence-specific nucleic acid sensor based on the blockage of a nanopore. Current methods for nucleic acid detection generally rely on polymerase chain reaction (PCR) and fluorescent labeling, however, these methods render the devices slow, expensive, complex, and bulky. In order to address these limitations, a new sensor was fabricated from a single glass wafer, consisting of a glass nanopore in a thin glass membrane. For nanopore sensing, low frequency noise is critical since it limits the discrimination of signal change based on target analyte movement from the fluctuation of noise. To further our understanding of nanopores, we observed how different pore geometries affect noise characteristics, and then compared this newly developed glass nanopore to conventional Si-based nanopores. Based on the analysis, low-noise glass nanopores, suitable for sequence-specific nucleic acid detection, were fabricated. By scaling down the pore diameter to the nano-regime, 1 aM detection of 16S rRNA from Escherichia coli was demonstrated even in the presence of a million-fold background of RNA from Pseudomona putida. This new platform for the PCR-free, optics-free, label-free sequence-specific nucleic acid detection shows the potential to detect pathogens in body fluids, food, or water. In addition, we developed a new method to transfer enzyme to a microelectrode array on an implantable microprobe, which enables fabrication of better performing microprobes for the sensing of multiple neurochemicals in vivo. Monitoring the release of neurotransmitters in real-time offers valuable information necessary to understand neurological disorders and abnormal behaviors. We employed polydimethylsiloxane (PDMS) stamping to transfer enzyme onto microelectrode array microprobes. A model enzyme, glucose oxidase (GOx), was stamped onto the surface of disk electrodes to test the feasibility of PDMS stamping for biosensor fabrication. The model sensor showed a good combination of performance (29 A/mM cm2 sensitivity and 4 M detection limit) proving that PDMS stamping offers a simple and cost-effective enzyme deposition method for construction of electroenzymatic sensors. The next step was to add an alignment function to PDMS stamping to create microprobes with dual sensing (glucose and choline) capabilities for in vivo applications. Two different enzymes, GOx and choline oxidase (ChOx), were selectively transferred onto specific sites in a 4 microelectrode array by PDMS stamping with alignment using a microscope and a custom-built stage. The dual sensor showed improved consistency and performance including sensitivity to choline and to glucose (286 and 117 A/mM cm2, respectively) as well as low detection limits (3 and 1 M, respectively). This work demonstrated the ability to immobilize specific enzymes on selected microelectrodes in an array to give a high performance microprobe for simultaneous sensing of two analytes for neuroscience application.

Author: Gérald Thouand
Publisher: Springer
ISBN: 9783030232160
Category : Science
Languages : en
Pages : 992

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Book Description
This handbook is an interdisciplinary and comprehensive reference covering all aspects of cell biosensors. It is divided into four main sections which are led and organized by numerous international experts. The scope of coverage includes: Fundamentals and genetics for biosensor applications Transducers, Materials and Systems Markets, innovation and education Application of biosensors in business Biosensor research is an exciting hybrid world where biologists, chemists, physicists, engineers and computer engineers come together. This handbook will serve as an invaluable living resource for all researchers in academia and industry working with cell biosensors.

Author: Mohd Azraai Mohd Razman
Publisher: Springer Nature
ISBN: 9811560250
Category : Computers
Languages : en
Pages : 285

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Book Description
This book highlights selected articles from the electrical engineering track, with a focus on the latest trends in electrical and electronic engineering toward embracing Industry 4.0, as part of the Malaysian Technical Universities Conference on Engineering and Technology—MUCET 2019. The event brings together researchers and professionals in the fields of engineering, research, and technology, and provides a platform for future collaborations and exchanges.

Author: Michael Koch
Publisher: Wiley-Interscience
ISBN: 9780863802447
Category : Technology & Engineering
Languages : en
Pages : 0

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Book Description
Discusses different modelling techniques in microfluidics (FEM and CFD). Every reader will have an easy start to model any kind of microfluidic device. Presents the necessary fabrication technologies and examples of the latest microfluidic devices and systems. Microfluidics is a very new research area in microelectro-mechanical systems (MEMS). This book introduces the theory and practice of microfluidic technology. The content is designed to be of value to engineers with different backgrounds working in the area of microsystem technology. The book includes the necessary fabrication technologies and examples of the latest microfluidic devices and systems that have been realised by a worldwide community of researchers. It covers all aspects of microfluidic theory and describes the breath-taking developments in this field.

Author: Chris D. Geddes
Publisher: John Wiley & Sons
ISBN: 0470642785
Category : Science
Languages : en
Pages : 655

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Book Description
Discover how metal-enhanced fluorescence is changing traditional concepts of fluorescence This book collects and analyzes all the current trends, opinions, and emerging hot topics in the field of metal-enhanced fluorescence (MEF). Readers learn how this emerging technology enhances the utility of current fluorescence-based approaches. For example, MEF can be used to better detect and track specific molecules that may be present in very low quantities in either clinical samples or biological systems. Author Chris Geddes, a noted pioneer in the field, not only explains the fundamentals of metal-enhanced fluorescence, but also the significance of all the most recent findings and models in the field. Metal-enhanced fluorescence refers to the use of metal colloids and nanoscale metallic particles in fluorescence systems. It offers researchers the opportunity to modify the basic properties of fluorophores in both near- and far-field fluorescence formats. Benefits of metal-enhanced fluorescence compared to traditional fluorescence include: Increased efficiency of fluorescence emission Increased detection sensitivity Protect against fluorophore photobleaching Applicability to almost any molecule, including both intrinsic and extrinsic chromophores Following a discussion of the principles and fundamentals, the author examines the process and applications of metal-enhanced fluorescence. Throughout the book, references lead to the primary literature, facilitating in-depth investigations into particular topics. Guiding readers from the basics to state-of-the-technology applications, this book is recommended for all chemists, physicists, and biomedical engineers working in the field of fluorescence.