Fabricating Paper-based Microfluidics with 3D Printing and PDMS Stamps PDF Download

Author: Robert Hunter Montgomery
Publisher:
ISBN:
Category : Microfluidics
Languages : en
Pages : 170

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Book Description

Author: Savas Tasoglu
Publisher: MDPI
ISBN: 3038974676
Category : Botanical chemistry
Languages : en
Pages : 213

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Book Description
This book is a printed edition of the Special Issue "3D Printed Microfluidic Devices" that was published in Micromachines

Author: Bryan Ellis
Publisher: CRC Press
ISBN: 1420005707
Category : Science
Languages : en
Pages : 1129

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Book Description
A reliable source for scientific and commercial information on over 1,000 polymers, this revised and updated edition features 25 percent new material, including 50 entirely new entries that reflect advances in such areas as conducting polymers, hydrogels, nano-polymers, and biomaterials. The second edition also comes with unlimited access to a complete, fully searchable web version of the reference. Powerful retrieval software allows users to customize their searches and refine results. Each entry includes trade names, properties, manufacturing processes, commercial applications, supplier details, references, and links to constituent monomers.

Author: Carlton A. McMullen
Publisher:
ISBN:
Category : Microfluidic devices
Languages : en
Pages : 174

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Book Description
A pneumatically actuated PDMS based microfluidic devices were designed and fabricated by soft-lithography. Two types of molds were fabricated out of different material for this experiment. The first mold, (device 1), was fabricated from a sheet of Polymethyl methacrylate (PMMA) material, similar to Plexiglas. The device features were micro-engraved onto the face of the material. The second mold, (device 2), was fabricated from the use of fused deposition modeling (FDM) 3D printing. The pumping efficiency of the PDMS devices was analyzed through the characterization of the micro-pumps flowrate with respect to the pumps driving pressure and the actuation frequency. Tested at a driving pressure of 10psi, the flowrate for device 1 peaked at 75μL/min with a 7Hz actuation frequency before failing, while device 2 peaked at 498μL/min with a 15Hz actuation frequency. Using the techniques of rapid prototyping and fused deposition modelling a pneumatically actuated 3D printer based micro-pump and micro-mixer are fabricated. The devices were fabricated using a thermoplastic elastomer (TPE) material as an alternative material to the present polydimethylsiloxane (PDMS). The micro-pump{u2019}s fluid flow output was analyzed through the characterization of the micro-pumps flowrate with respect to the pumps driving pressure and the actuation frequency. Testing showed that a maximum flowrate of 1120μL/min was achieved at an actuation frequency of 10Hz with an applied driving pressure of 40psi. A qualitative mixing performance was conducted with the micro-mixer. The diffusion of two dyes was tested under an active mix and non-active mix condition. Testing showed that the active mixing condition resulted in a complete diffusion as opposed to the non-mixing condition which partially diffused. As a proof of concept for biological testing, E. coli and E.coli anti-bodies were mixes to measure the capturing efficiency. The results showed that the active mixing resulted in about 50% capturing efficiency as opposed to the non-mixing which resulted in 33% capturing efficiency.

Author: Guihe Li
Publisher:
ISBN:
Category : Biomedical materials
Languages : en
Pages : 122

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Book Description
Microfluidics, the study and utilization of fluids on micron length scales, is a rapidly growing field with applications in various areas including molecular biology, drug screening, chemical synthesis, and cell biology. Researchers are able to take advantage of unique physical characteristics to improve mass and heat transfer and achieve, for example, rapid diffusion and laminar flow. Consequently, spatial microenvironments can be precisely controlled within microfluidic devices. Polydimethylsiloxane (PDMS), a silicone-based elastomer, has been widely used to fabricate microfluidic devices because of its excellent optical clarity, high gas permeability, and biocompatibility. The combination of gas transport and fluid flow within PDMS microfluidic devices is crucial for many biomedical experiments because local concentrations may significantly affect spatially localized conditions and cell behavior. One consequential example is the fabrication of hydrogel microparticles and the encapsulation of cells within them, both of which are dramatically affected by subtle variations in oxygen concentration. Here, we have designed and fabricated an optimized microfluidic device with which we have conducted a systematic study to understand gas exchange through PDMS and its effect on microgel fabrication within microdroplets. Based on this study, a new microfabrication technique, which combines 3D printing and soft lithography for multi-depth microfluidic devices has been developed. A transformative approach to PDMS microfabrication is presented that transcends mask-based photolithography barriers and allows the fabrication of multi-depth devices in an alignment-free and low-cost manner. We believe that the presented 3D-printing method can overcome limitations of soft lithography and enable additional applications for multi-layer PDMS microfluidic devices.

Author: Andreas Manz
Publisher: Royal Society of Chemistry
ISBN: 1782628339
Category : Science
Languages : en
Pages : 307

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Book Description
Responding to the need for an affordable, easy-to-read textbook that introduces microfluidics to undergraduate and postgraduate students, this concise book will provide a broad overview of the important theoretical and practical aspects of microfluidics and lab-on-a-chip, as well as its applications.

Author: Madhulika Pradhan
Publisher: CRC Press
ISBN: 1040131875
Category : Medical
Languages : en
Pages : 439

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Book Description
3D Printing and Microfluidics in Dermatology provides a thorough exploration and applications of three-dimensional (3D) printing and microfluidics within the field of dermatology. It investigates various methods utilized in these fields, such as 3D bioprinting, nano-transporters, microscopic fabrication, and device development. The book not only examines practical applications but also delves into the design principles crucial for implementing these techniques using specific materials tailored to their intended purposes. Additionally, it addresses ethical concerns and regulatory considerations pertinent to these evolving technologies. Key highlights include the following: A detailed insight into the utilization of 3D printing and microfluidic technologies for treating skin disorders. Exploration of design concepts necessary for effective implementation, considering the unique properties of materials involved. Coverage of diverse methodologies, ranging from 3D bioprinting to nano-transporters, microscopic fabrication, and device engineering. In-depth discussion on ethical considerations vital for the sustainable development of the industry. Investigation into advancements in material development, device design, fabrication techniques, and performance evaluation through preclinical and clinical studies. This book targets graduate students and researchers in fields such as 3D printing, dermatology, drug delivery, bioengineering, and pharmaceutical sciences.

Author: Tasoglu Savas
Publisher:
ISBN: 9783038974680
Category : Electronic books
Languages : en
Pages : 1

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Book Description
3D printing has revolutionized the microfabrication prototyping workflow over the past few years. With the recent improvements in 3D printing technologies, highly complex microfluidic devices can be fabricated via single-step, rapid, and cost-effective protocols as a promising alternative to the time consuming, costly and sophisticated traditional cleanroom fabrication. Microfluidic devices have enabled a wide range of biochemical and clinical applications, such as cancer screening, micro-physiological system engineering, high-throughput drug testing, and point-of-care diagnostics. Using 3D printing fabrication technologies, alteration of the design features is significantly easier than traditional fabrication, enabling agile iterative design and facilitating rapid prototyping. This can make microfluidic technology more accessible to researchers in various fields and accelerates innovation in the field of microfluidics. Accordingly, this Special Issue seeks to showcase research papers, short communications, and review articles that focus on novel methodological developments in 3D printing and its use for various biochemical and biomedical applications.

Author: Xiujun (James) Li
Publisher: Newnes
ISBN: 0444594612
Category : Science
Languages : en
Pages : 486

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Book Description
Multidisciplinary Microfluidic and Nanofluidic Lab-on-a-Chip: Principles and Applications provides chemists, biophysicists, engineers, life scientists, biotechnologists, and pharmaceutical scientists with the principles behind the design, manufacture, and testing of life sciences microfluidic systems. This book serves as a reference for technologies and applications in multidisciplinary areas, with an emphasis on quickly developing or new emerging areas, including digital microfluidics, nanofluidics, papers-based microfluidics, and cell biology. The book offers practical guidance on how to design, analyze, fabricate, and test microfluidic devices and systems for a wide variety of applications including separations, disease detection, cellular analysis, DNA analysis, proteomics, and drug delivery. Calculations, solved problems, data tables, and design rules are provided to help researchers understand microfluidic basic theory and principles and apply this knowledge to their own unique designs. Recent advances in microfluidics and microsystems for life sciences are impacting chemistry, biophysics, molecular, cell biology, and medicine for applications that include DNA analysis, drug discovery, disease research, and biofluid and environmental monitoring. Provides calculations, solved problems, data tables and design rules to help understand microfluidic basic theory and principles Gives an applied understanding of the principles behind the design, manufacture, and testing of microfluidic systems Emphasizes on quickly developing and emerging areas, including digital microfluidics, nanofluidics, papers-based microfluidics, and cell biology

Author: Casey Carter Glick
Publisher:
ISBN:
Category :
Languages : en
Pages : 302

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Book Description
Microfluidics, the science and engineering of fluid at small scales, affords numerous benefits for applications in chemistry and biology, including rapid reaction rates, reaction uniformity and precision, and reagent minimization but the technology remains limited by the availability of appropriate control mechanisms and related microfluidic components. Microfluidic devices have traditionally been fabricated using soft-lithography, which is time-consuming, costly, and reliant on extensive facilities. Over the past decade, research has shifted towards developing alternate methods such as additive manufacturing (widely known as three-dimensional (3D) printing) to fabricate microfluidic structures. This dissertation has developed three methods for resolving the fabrication and control problems of microfluidics using a combination of standard microfluidic/MEMS techniques with newer 3D printing techniques: optofluidic lithographic microfluidic circuitry, 3D-printed transfer molding, and fully-3D-printed fluidic circuitry. The basic theory of microfluidic control systems, including the hydraulic analogy and fluidic circuitry, the low Reynolds number approximation to the Navier-Stokes equations, and neglectable terms in microfluidic models are first investigated. Optofluidic lithography, where photocurable liquids are selectively solidified under UV exposure, is utilized as a means of fabricating moving microfluidic structures within a microchannel. Several variants of microfluidic control mechanisms are demonstrated, including current sources (for regulation of fluid flow-rates to $29.2 \pm 0.8$ \si{\micro\liter/min} for pressures from $5-10$ kPa), and microfluidic gain valves (for controlling a high-pressure channel with a low-pressure one to achieve a dynamic gain of $6.30 \pm 0.23$). A hybrid manufacturing process, where 3D printed polymer molds are used to make multi-layer PDMS constructions by employing unique molding, bonding, alignment, and rapid assembly processes. Specifically, a novel single-layer, two-sided molding method is developed to realize two channels levels, non-planar membranes/valves, vertical interconnects (vias) between channel levels, and built-in inlet/outlet ports for fast linkages to external fluidic systems. As a demonstration, a single-layer membrane micro-valve is constructed, dramatically reducing the number of fabrication steps. Additionally, multilayer structures are fabricated through an intra-layer bonding procedure which uses custom 3D printed stamps to selectively apply uncured liquid PDMS adhesive only to bonding interfaces without clogging fluidic channels. Using built-in alignment marks to accurately position both stamps and individual layers, this technique is demonstrated by rapidly assembling a six-layer microfluidic device. By using the multi-jet-based additive manufacturing, fully-3D-printed microfluidic circuitry components are demonstrated, including fluidic resistors, capacitors, diodes (for a diodicity of $80.6 \pm 1.8$), and transistors (for a dynamic pressure gain of $3.01 \pm 0.78$) through the combination of experimental, simulation, and analytical methods, which are integrated complex microfluidic circuits, such as half- and full-wave microfluidic rectifiers. The hydraulic analogy circuitry exhibits similar transfer functions to its electronic counterparts, but ultimately behaves more similarly to vacuum triodes than to actual transistors. When combined with fully-realized microfluidic IF-gate and CMOS-analog components, this research may allow for fabrication of analog microfluidic operational-amplifiers, which could allow for fully-analog control of microfluidic systems using high-gain amplification of small pressure signals, and the CAD-based nature of the design process makes it simple for researchers to readily combine multiple microfluidic components into larger integrated microfluidic circuit networks.