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Bioconjugate Techniques

Bioconjugate Techniques PDF Author: Greg T. Hermanson
Publisher: Academic Press
ISBN: 0080568726
Category : Science
Languages : en
Pages : 1233

Book Description
Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates Includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticlesSilane coupling agentsDendrimers and dendronsChemoselective ligationQuantum dotsLanthanide chelatesCyanine dyesDiscrete PEG compoundsBuckyballs,fullerenes, and carbon nanotubesMass tags and isotope tagsBioconjugation in the study of protein interactions

Bioconjugate Techniques

Bioconjugate Techniques PDF Author: Greg T. Hermanson
Publisher: Academic Press
ISBN: 0080568726
Category : Science
Languages : en
Pages : 1233

Book Description
Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates Includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticlesSilane coupling agentsDendrimers and dendronsChemoselective ligationQuantum dotsLanthanide chelatesCyanine dyesDiscrete PEG compoundsBuckyballs,fullerenes, and carbon nanotubesMass tags and isotope tagsBioconjugation in the study of protein interactions

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ISBN: 1483194884
Category : Science
Languages : en
Pages : 345

Book Description
Physical Techniques in Biological Research, Second Edition, Volume II, Part B: Physical Chemical Techniques covers physical chemical techniques that have been most widely employed in the investigation of molecules of biological significance. The book discusses the measurement and properties of ionizing radiations; magnetic resonance and related techniques in magnetic susceptibility measurements; and chromatographic methods and applications of chromatography to steroid research. The text then describes the phenomenon of centrifugation, including the techniques for studying density gradient centrifugation; the formation of gradient column in swinging-bucket tubes; gradient materials; density gradient centrifugation in heavy salt; some factors influencing the distribution of sedimenting particles; and the recovery of samples. Biological investigators and students of biophysics or biochemistry will find the book invaluable.

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