Electrophysiology of Isolated Mammalian CNS Preparations PDF Download

Author: G. A. Kerkut
Publisher:
ISBN:
Category : Brain chemistry
Languages : en
Pages : 402

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Author: Abel Lajtha
Publisher: Springer Science & Business Media
ISBN: 1468470183
Category : Science
Languages : en
Pages : 686

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Author: P. Michael Conn
Publisher: Elsevier
ISBN: 1483284891
Category : Science
Languages : en
Pages : 521

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Book Description
Electrical activity is central to the functioning and nature of neural cells. Every effort has been made in this volume to provide representative examples of useful techniques that have made this area a productive component of the neurosciences. Convenient bench-top format**Methods presented for easy adaptation to new systems**Comprehensive protocols included for**Cell recording: from conscious and freely behaving animals, measurement of calcium currents, and multiple electrodes**Voltage clamping and voltammetry: apparatus and applications**Specific ion channels and receptors: expression in oocytes, single-cell secretion, purification of calcium channel drug receptors**Special electrodes and other equipment: movable electrodes, microelectrodes, multiple electrodes, en passant recording, microincubators for optical studies**Special preparations: multicompartment cell cultures, grease-gap methods, perforated path techniques, giant proteoliposomes for reconstitution studies, microinjection**Data analysis and reduction: digital filtering on a personal computer, filtering on a personal computer, fitting of single-channel dwell time distributions, discrimination of kinetic models, voltage noise analysis, list-oriented analysis of single-channel systems

Author: Paul J. Marangos
Publisher: Elsevier
ISBN: 0323152805
Category : Science
Languages : en
Pages : 384

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Brain Receptor Methodologies, Part A, General Methods and Concepts: Amines and Acetylcholine provides information pertinent to neurotransmitter and neuromodulator receptors in brain. This book explores the methodologies that can used to address several basic and clinical problems. Organized into two sections encompassing 18 chapters, this book starts with an overview of the receptor concept, which can be validated from indirect evidence obtained in studies of the quantitative aspects of drug antagonism. This text then examines the radioligand–receptor binding interactions. Other chapters consider immunocytochemistry, which has a primary role in determining the precise distribution of regulatory peptides to neural and endocrine elements of the diffuse neuroendocrine system. The final chapter discusses the use of the radioligand binding procedure for the study of muscarinic receptors, which has expanded the area of muscarinic receptor pharmacology. Biochemists, pharmacologists, physiologists, and researchers engaged in the fields of neurobiology and neuroscience will find this book extremely useful.

Author: N. I. A. Rakkah
Publisher:
ISBN:
Category :
Languages : en
Pages :

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Author: Kenneth Blum
Publisher: CRC Press
ISBN: 9780824772833
Category : Medical
Languages : en
Pages : 708

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Author: Leslie L. Iversen
Publisher: Springer Science & Business Media
ISBN: 1461334527
Category : Medical
Languages : en
Pages : 359

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Volume 15 of Handbook of Psychopharmacology represents the first of a new series of volumes whose aim is to bring earlier sections of the work up to date by describing the latest developments in the field. It is now seven years since the first Handbook volumes on Basic Neuropharmacology were published, and there have been many important advances. As in many other areas in science, progress in this field has depended to a considerable extent on the availability of new experimental methods, and Volume 15 reviews some major recent developments, including new autoradiographic techniques that allow direct visualization of drug and transmitter receptors in the nervous system, and the pinpointing of the precise locations of the changes in brain metabolism elicited by various drug treatments. Volumes 16 and 17 will cover two of the most active areas for basic research in psychopharmacology at the moment: the characterization of drug and transmitter receptors in brain by radioligand binding techniques, and studies of the role of small peptides in brain function. The latter area, in particular, illustrates how rapidly progress continues to be made in basic research on the mechanisms of chemical communication within the nervous system. Seven years ago when the Handbook first appeared none of the opioid peptides (enkephalins and endorphins) had yet been identified. Since then a whole new area of basic biological research has focused on these substances, and in addition we know of more than thirty other neuropeptides with putative eNS transmitter functions.

Author: Henrik Jahnsen
Publisher:
ISBN:
Category : Medical
Languages : en
Pages : 264

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Presents some of the latest in vitro techniques that can be used to study the vertebrate central nervous system--particularly the brain slice technique. The advent of this new era in neuroscience led to a number of difficult test limitations in the use of this technique, including problems associated with the study of properties in large three-dimensional neural networks and processes lasting longer than 18-24 hours. The authors present solutions to these problems and indicate how it is possible to push in vitro techniques toward their known limits. Invaluable, this work will serve as a stepping-stone to further research and development activity in the neuroscience field.

Author: Sten Grillner
Publisher: Springer
ISBN: 1349091480
Category : Science
Languages : en
Pages : 719

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Author: Raymond Dingledine
Publisher: Springer Science & Business Media
ISBN: 1468445839
Category : Medical
Languages : en
Pages : 452

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Book Description
In little less than a decade brain slices have gained prominence among neurobiologists as appropriate tools to study cellular electrophysiolog ical aspects of mammalian brain function. The purpose of this volume is to present in some detail several inquiries in the brain sciences that have benefited greatly by the use of brain slices. The book is directed primarily toward advanced students and researchers wishing to evaluate the impact these in vitro preparations of the mammalian brain are having on neurobiology. The term brain slice has come to refer to thin (100-700 j. Lm) sections of a brain region prepared from adult mammals and maintained for many hours in vitro, for either electrophysiological or biochemical stud ies. In addition to good accessibility, slices feature relatively intact syn aptic connections that allow a variety of experiments not feasible with standard in vivo or tissue culture preparations. Certain electrophysiol ogical studies once practical only with invertebrate models are becoming routine with mammalian brain slices. The ability to perform both bio chemical and electro physiological experiments on the same piece of CNS tissue provides additional bright prospects for future research. Although most of the electrophysiological studies have dealt with hippocampal slices, it should be evident from this book that slice methodology is not limited to the hippocampus. The Appendix, "Brain Slice Methods," is a multiauthored treatment of the technical aspects of brain slice work, collected into one document.