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Author: P. Michael Conn Publisher: Elsevier ISBN: 1483284891 Category : Science Languages : en Pages : 521
Book Description
Electrical activity is central to the functioning and nature of neural cells. Every effort has been made in this volume to provide representative examples of useful techniques that have made this area a productive component of the neurosciences. Convenient bench-top format**Methods presented for easy adaptation to new systems**Comprehensive protocols included for**Cell recording: from conscious and freely behaving animals, measurement of calcium currents, and multiple electrodes**Voltage clamping and voltammetry: apparatus and applications**Specific ion channels and receptors: expression in oocytes, single-cell secretion, purification of calcium channel drug receptors**Special electrodes and other equipment: movable electrodes, microelectrodes, multiple electrodes, en passant recording, microincubators for optical studies**Special preparations: multicompartment cell cultures, grease-gap methods, perforated path techniques, giant proteoliposomes for reconstitution studies, microinjection**Data analysis and reduction: digital filtering on a personal computer, filtering on a personal computer, fitting of single-channel dwell time distributions, discrimination of kinetic models, voltage noise analysis, list-oriented analysis of single-channel systems
Author: P. Michael Conn Publisher: Elsevier ISBN: 1483284891 Category : Science Languages : en Pages : 521
Book Description
Electrical activity is central to the functioning and nature of neural cells. Every effort has been made in this volume to provide representative examples of useful techniques that have made this area a productive component of the neurosciences. Convenient bench-top format**Methods presented for easy adaptation to new systems**Comprehensive protocols included for**Cell recording: from conscious and freely behaving animals, measurement of calcium currents, and multiple electrodes**Voltage clamping and voltammetry: apparatus and applications**Specific ion channels and receptors: expression in oocytes, single-cell secretion, purification of calcium channel drug receptors**Special electrodes and other equipment: movable electrodes, microelectrodes, multiple electrodes, en passant recording, microincubators for optical studies**Special preparations: multicompartment cell cultures, grease-gap methods, perforated path techniques, giant proteoliposomes for reconstitution studies, microinjection**Data analysis and reduction: digital filtering on a personal computer, filtering on a personal computer, fitting of single-channel dwell time distributions, discrimination of kinetic models, voltage noise analysis, list-oriented analysis of single-channel systems
Author: Publisher: ScholarlyEditions ISBN: 148167420X Category : Science Languages : en Pages : 948
Book Description
Cellular Structures—Advances in Research and Application: 2013 Edition is a ScholarlyEditions™ book that delivers timely, authoritative, and comprehensive information about Intracellular Space. The editors have built Cellular Structures—Advances in Research and Application: 2013 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Intracellular Space in this book to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Cellular Structures—Advances in Research and Application: 2013 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Author: Hans Scherubl Publisher: CRC Press ISBN: 9780849324772 Category : Science Languages : en Pages : 388
Book Description
The Electrophysiology of Neuroendocrine Cells explores the role of electrical activity in neuroendocrine cells in stimulus-secretion coupling, sensory mechanisms, and intercellular communication. This comprehensive and concise handbook includes introductory material on the ontogenesis and classification of the neuroendocrine system and describes general electrical properties, voltage-gated ion channels, and the pharmacology of ion channels. By focusing on functional aspects, The Electrophysiology of Neuroendocrine Cells provides research scientists, physicians, and students with a basic understanding of neuroendocrine cells and their similarity to neurones, as well as their relationship to thyroid- or steroid-hormone secreting endocrine cells. The multidisciplinary nature of this book provides readers with a broad perspective on the electrical properties of neuroendocrine cells, and the combination of general information and specialized information makes the book accessible to beginning and advanced readers alike.
Author: Xinyu Liu Publisher: Academic Press ISBN: 0128242434 Category : Business & Economics Languages : en Pages : 406
Book Description
Micro and Nano Systems for Biophysical Studies of Cells and Small Organisms provides a comprehensive introduction to the state-of-the-art micro and nano systems that have recently been developed and applied to biophysical studies of cells and small organisms. These micro and nano systems span from microelectromechanical systems (MEMS) and microfluidic devices to robotic micro-nanomanipulation systems. These biophysical studies range from cell mechanics to the neural science of worms and Drosophila. This book will help readers understand the fundamentals surrounding the development of these tools and teach them the most recent advances in cellular and organismal biophysics enabled by these technologies. Comprehensive coverage of micro and nano-system technology and application to biophysical studies of cells and small organisms. Highlights the most recent advances in cellular and organismal biophysics enabled by micro and nano systems. Insightful outlook on future directions and trends in each chapter covering a sub-area of the book topic.
Author: Angel Cid-Arregui Publisher: Springer Science & Business Media ISBN: 3642803431 Category : Science Languages : en Pages : 619
Book Description
The establishment of microinjection protocols about 20 years ago for cultured cells and shortly thereafter for the generation of transgenic mice by microinjection of DNA into fertilized mouse eggs greatly influ enced many fields of biology. Not only have the data generated using these approaches contributed to a large extent to our present under standing of gene regulation and cellular function of higher eukaryotic cells, but current knowledge and future developments in this area will certainly have a great impact on basic and applied research for many years to come. This laboratory manual describes the current state of the art in this research area and focuses primarily on both the experimental strategies with an extensive bibliography and the detailed procedures. A large number of studies are presently being performed and a great variety of new experimental designs are rapidly being developed. The book con tains protocols on injection of somatic cells as well as on injection of embryos, the use of similar equipment being a common feature. In the articles dedicated to somatic cells, full descriptions of the manual and automatic injection systems are given as well as the methods for the analysis of injected cells by video-microscopy, electron microscopy or in situ hybridizations. In addition, comprehensive protocols are given for injection experiments with very different purposes, such as to study sig nal transduction or microtubule dynamics.
Author: Tuhin Subhra Santra Publisher: MDPI ISBN: 3036506284 Category : Science Languages : en Pages : 254
Book Description
Cells are the most fundamental building block of all living organisms. The investigation of any type of disease mechanism and its progression still remains challenging due to cellular heterogeneity characteristics and physiological state of cells in a given population. The bulk measurement of millions of cells together can provide some general information on cells, but it cannot evolve the cellular heterogeneity and molecular dynamics in a certain cell population. Compared to this bulk or the average measurement of a large number of cells together, single-cell analysis can provide detailed information on each cell, which could assist in developing an understanding of the specific biological context of cells, such as tumor progression or issues around stem cells. Single-cell omics can provide valuable information about functional mutation and a copy number of variations of cells. Information from single-cell investigations can help to produce a better understanding of intracellular interactions and environmental responses of cellular organelles, which can be beneficial for therapeutics development and diagnostics purposes. This Special Issue is inviting articles related to single-cell analysis and its advantages, limitations, and future prospects regarding health benefits.
Author: Jay L. Nadeau Publisher: CRC Press ISBN: 1351000926 Category : Medical Languages : en Pages : 1223
Book Description
Praise for the First Edition “essential reading for any physical scientist who is interested in performing biological research.” ―Contemporary Physics "an ambitious text.... Each chapter contains protocols and the conceptual reasoning behind them, which is often useful to physicists performing biological experiments for the first time." –Physics Today This fully updated and expanded text is the best starting point for any student or researcher in the physical sciences to gain firm grounding in the techniques employed in molecular biophysics and quantitative biology. It includes brand new chapters on gene expression techniques, advanced techniques in biological light microscopy (super-resolution, two-photon, and fluorescence lifetime imaging), holography, and gold nanoparticles used in medicine. The author shares invaluable practical tips and insider’s knowledge to simplify potentially confusing techniques. The reader is guided through easy-to-follow examples carried out from start to finish with practical tips and insider’s knowledge. The emphasis is on building comfort with getting hands "wet" with basic methods and finally understanding when and how to apply or adapt them to address different questions. Jay L. Nadeau is a scientific researcher and head of the Biomedical Engineering in Advanced Applications of Quantum, Oscillatory, and Nanotechnological Systems (BEAAQONS) lab at Caltech and was previously associate professor of biomedical engineering and physics at McGill University.
Author: Publisher: Elsevier ISBN: 0080536476 Category : Mathematics Languages : en Pages : 449
Book Description
In this volume contemporary methods designed to provide insights into, mathematical structure for, and predictive inferences about neuroendocrine control mechanisms are presented. Collates an array of contemporary techniques for analysis of neuroendocrine data Discusses current problems in and solutions to neurohormone pulse analysis Identifies relevant software available
Author: P. Michael Conn
Author: P. Michael Conn
Author: P. Michael Conn
Author: P. Michael Conn
Author: 
Author: Hans Scherubl
Author: Xinyu Liu
Author: Angel Cid-Arregui
Author: Tuhin Subhra Santra
Author: Jay L. Nadeau
Author: