Electrophoretic Separation of Proteins

Electrophoretic Separation of Proteins PDF Author: Biji T. Kurien
Publisher:
ISBN: 9781493987931
Category : Electrophoresis
Languages : en
Pages : 523

Book Description


Molecular Biology of the Cell

Molecular Biology of the Cell PDF Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0

Book Description


Capillary Gel Electrophoresis

Capillary Gel Electrophoresis PDF Author: Andras Guttman
Publisher: Newnes
ISBN: 0080931359
Category : Science
Languages : en
Pages : 391

Book Description
Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. - Covers all theoretical and practical aspects of capillary gel electrophoresis - Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods - Teaches readers how to use the technology and select methods that are ideal for fundamental problems - Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses

Electrophoresis in the Separation of Biological Macromolecules

Electrophoresis in the Separation of Biological Macromolecules PDF Author: Ö Gaal
Publisher: John Wiley & Sons
ISBN:
Category : Science
Languages : en
Pages : 432

Book Description
Theorical and technical bases of electrophoretic methods; Principles of electrophoresis; Moving boundary electrophoresis; Eletrophoresis of proteins; Electrophoresis bahavior of proteins; Separation of proteins according to their molecular size: estimation of molecular weights; Two-dimensional electrophoretic techniques; Staining of proteins; Detection of proteins; Electrophoretic separation of certain groups of proteins; Electrophoresis of nucleic acids and nucleoproteins; Estimation of the molecular weight of polynucleotides; Electrophoretic separtion of glycosaminoglycans.

RNA Methodologies

RNA Methodologies PDF Author: Robert E. Farrell Jr.
Publisher: Elsevier
ISBN: 0080454763
Category : Science
Languages : en
Pages : 794

Book Description
This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project.RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5' and 3' RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis.* Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects

Experiments in the Purification and Characterization of Enzymes

Experiments in the Purification and Characterization of Enzymes PDF Author: Thomas E. Crowley
Publisher: Academic Press
ISBN: 0124095933
Category : Science
Languages : en
Pages : 267

Book Description
Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual provides students with a working knowledge of the fundamental and advanced techniques of experimental biochemistry. Included are instructions and experiments that involve purification and characterization of enzymes from various source materials, giving students excellent experience in kinetics analysis and data analysis. Additionally, this lab manual covers how to evaluate and effectively use scientific data. By focusing on the relationship between structure and function in enzymes, Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual provides a strong research foundation for students enrolled in a biochemistry lab course by outlining how to evaluate and effectively use scientific data in addition to offering students a more hands-on approach with exercises that encourage them to think deeply about the content and to design their own experiments. Instructors will find this book useful because the modular nature of the lab exercises allows them to apply the exercises to any set of proteins and incorporate the exercises into their courses as they see fit, allowing for greater flexibility in the use of the material. Written in a logical, easy-to-understand manner, Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual is an indispensable resource for both students and instructors in the fields of biochemistry, molecular biology, chemistry, pharmaceutical chemistry, and related molecular life sciences such as cell biology, neurosciences, and genetics. - Offers project lab formats for students that closely simulate original research projects - Provides instructional guidance for students to design their own experiments - Includes advanced analytical techniques - Contains adaptable modular exercises that allow for the study proteins other than FNR, LuxG and LDH - Includes access to a website with additional resources for instructors

Viroids and Satellites

Viroids and Satellites PDF Author: Ahmed Hadidi
Publisher: Academic Press
ISBN: 0128017023
Category : Science
Languages : en
Pages : 754

Book Description
Viroids and Satellites describes plant diseases and their causal agents while also addressing the economic impact of these diseases. The book discusses various strategies for state-of-the-art methods for the detection and control of pathogens in their infected hosts and provides pivotal information from the discovery of viroids through the analysis of their molecular and biological properties, to viroid pathogenesis, host interactions, and RNA silencing pathways. Students, researchers and regulators will find this to be a comprehensive resource on the topics presented. - Provides coverage of the basic biological properties of disease, along with applied knowledge - Features economic impacts, transmission, geographical distribution, epidemiology, detection, and control within each chapter - Organizes viroid diseases by viroid taxonomy and viroid species

Paper Electrophoresis

Paper Electrophoresis PDF Author: G. E. W. Wolstenholme
Publisher: John Wiley & Sons
ISBN: 0470716452
Category : Science
Languages : en
Pages : 263

Book Description
The Novartis Foundation Series is a popular collection of the proceedings from Novartis Foundation Symposia, in which groups of leading scientists from a range of topics across biology, chemistry and medicine assembled to present papers and discuss results. The Novartis Foundation, originally known as the Ciba Foundation, is well known to scientists and clinicians around the world.

Food Protein Chemistry

Food Protein Chemistry PDF Author: Joe Regenstein
Publisher: Elsevier
ISBN: 0323153860
Category : Technology & Engineering
Languages : en
Pages : 368

Book Description
Food Protein Chemistry: An Introduction for Food Scientists discusses food proteins and how they are studied. Proteins are both biological entities and physicochemical compounds, and they will be examined in both contexts in this volume. The chemical and physical properties of proteins will be viewed from the perspective of chemists despite the fact that their use in the food supply emphasizes their biological nature. Key topics discussed include proteins as essential to life; amino acids; protein classification; selected proteins of the most important food systems; and protein structure. The book also includes chapters on protein measurement; protein purification; and spectral techniques for the study of proteins. The book requires readers to have the equivalent of the Institute of Food Technologists requirements for undergraduate food science majors. It also assumes a knowledge of math through calculus. While primarily intended for senior and first-year graduate food science students, the text may also be useful to researchers in allied fields.

Electrophoresis in Practice

Electrophoresis in Practice PDF Author: Reiner Westermeier
Publisher: John Wiley & Sons
ISBN: 3527338802
Category : Science
Languages : en
Pages : 470

Book Description
"Electrophoresis in Practice" ist seit mehr als zwei Jahrzehnten das Standardwerk in der Elektrophorese. Die 5. Auflage wurde sorgfältig überarbeitet und beinhaltet nun ein erweitertes Kapitel zu Mikromethoden und der chipgebundenen Elektrophorese.