Author: J. F. AlksnePublisher:
ISBN: 9783662304518
Category :
Languages : en
Pages : 36
Book Description
Electron Microscopy of Axon Degeneration
Author: J. F. AlksnePublisher:
ISBN: 9783662304518
Category :
Languages : en
Pages : 36
Book Description
Electron Microscopy of Axon Degeneration: A Valuable Tool in Experimental Neuroanatomy
Author: J.F. AlksnePublisher: Springer
ISBN: 3662304503
Category : Medical
Languages : en
Pages : 33
Book Description
Experimental methods for the mapping of nervous pathways are based partlyon the study of retrograde processes in the perikaryon, partlyon the demonstration of degenerative processes along the peripheral part of a transected axon. For this purpose, the Marchi method by which a selective staining of degenerating myelin is obtained has been extensively used. However, when this method is used the non-myelinated terminals of the transected axons are not stained. The introduction, about two decades ago, of silver impregnation as a means of tracing degenerating axons (especially the Glees and Nauta methods) by which also terminal boutons can be demonstrated, led therefore to revolutionary progress in the investigation of interneuronal connections. Notwithstanding, there are weH known difficulties involved in this kind of research. The capriciousness of the silver methods not seldom results in failure of impregnation with loss of valuable experimental animals. But even when well impregnated sections are used, other fundamental difficulties exist. One of the major problems is to prove beyond doubt that the impregnated structures are degenerating boutons and not merely fragments of non-terminal fibres passing the area under examination. Furthermore, only on occasion will silver impregnation permit one to accurately define the specific part of the receiving neuron on which the impregnated fibres end, i. e. , whether the bouton makes contact with soma, dendrite or spine.
Electron Microscopy of Axon Degeneration
Author: Electron Microscopy of Axon Degeneration
Author: John F. AlksneElectron Microscopy of Axon Degeneration
Author: Axon Degeneration
Author: Elisabetta BabettoPublisher: Humana
ISBN: 9781071605844
Category : Science
Languages : en
Pages : 0
Book Description
This book is a collection of classical as well as innovative methods used to investigate axon degeneration with a particular focus on addressing the common challenges encountered while performing these procedures. Particular attention is devoted to the study of axon loss in several model organisms, as each poses unique challenges and provides powerful advantages. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Axon Degeneration: Methods and Protocols is an ideal guide for facilitating the application and further development of these protocols, which will help the scientific community tackle important questions regarding axon degeneration. Chapters 2, 3, and 20 are available Open Access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Contemporary Research Methods in Neuroanatomy
Author: Walle J.H. NautaPublisher: Springer
ISBN: 9783642859885
Category : Medical
Languages : en
Pages : 0
Book Description
As a source of information on neuroanatomical research methods this Volume is not without precedent. In 1957, at the initiative of Dr. W. F. Windle, a conference was held at the National Institutes of Health, the proceedings of which, edited by Dr. Windle and published by C. C. Thomas under the title "New Research Tech niques of Neuroanatomy", rapidly became something like a standard reference in the field of Neuromorphology. The present editors were emboldened to seek support for a second expose of contemporary research methods in Neuroanatomy by the success of this earlier publication, as well as by the consideration that the years elapsed since its appearance have been, perhaps, more productive of new research methods and strategies in Neuroanatomy than were any dozen consecutive years since the golden decades of the 1870's and 1880's. The decision, which methods to include in this conference, has been a difficult one. For reasons of space alone it would have been impossible to do equal justice to techniques approaching the brain as a neuronal system, the brain as a tissue, or the neuron as a cell. As a brief inspection of the contents of this volume will show, the weight of choice fell upon the first of these alternatives. The reader will find, further more, that not all of the book is devoted to new methods in the strict sense.
Axonal Transport, Degeneration, and Regeneration in the Visual System of the Goldfish
Author: Hartwig WolburgPublisher: Springer
ISBN: 9783642678011
Category : Medical
Languages : en
Pages : 0
Book Description
References ............................... 76 Subject Index ............................. 93 VIII Acknowledgments This study was funded by the Deutsche Forschungsgemeinschaft. I am indebted to Prof. Dr. W. Schlote for helpful advice and numerous discussions. I am also grateful to Dr. G. Kurz-Isler for her generous help in problems dealing with electron microscopy and to Mrs. B. Sabrowski for her careful preparation of the manuscript. The careful translation of T.C. Telger is gratefully acknowledged. The translation was financially supported by the Erwin Riesch Foundation. IX 1 Introduction One of the basic principles underlying the efficiency and adaptability of cellular meta bolism is the structural compartmentalization of the cell. Only through compartmenta lization can reaction components be kept apart prior to their reaction, isolated from other "reaction spaces" during the course of their reaction, and the reaction products incorporated into designated structures or transported to remote parts of the cell. Thus, the partitioning of the cellular substance into countless membranous spaces corresponds to the spatial segregation of reaction components, and the dynamics of intracellular membrane systems is an expression of ever-changing equilibrium condi tions and the continuous formation of new reaction spaces. It has been shown with some certainty that many of the processes in membrane dynamics can take place only with the aid of contractile proteins such as actin, myosin, and tubulin.
Changes at the nodes of Ranvier during Wallerian degeneration: an electron microscope study
Author: Rosalind Helen Mary BallinPublisher:
ISBN:
Category : Signature
Languages : en
Pages : 0
Book Description
Neuroproteomics
Author: Oscar AlzatePublisher: CRC Press
ISBN: 1420076264
Category : Medical
Languages : en
Pages : 356
Book Description
In this, the post-genomic age, our knowledge of biological systems continues to expand and progress. As the research becomes more focused, so too does the data. Genomic research progresses to proteomics and brings us to a deeper understanding of the behavior and function of protein clusters. And now proteomics gives way to neuroproteomics as we beg