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Electron Microscopy of Plant Cells

Electron Microscopy of Plant Cells PDF Author: C Hawes
Publisher: Academic Press
ISBN: 0323148980
Category : Science
Languages : en
Pages : 477

Book Description
Electron Microscopy of Plant Cells serves as manual or reference of major modern techniques used to prepare plant material for transmission and scanning electron microscopy. There have been other books that generally discuss electron microscope methodology. This book focuses on problem areas encountered through the presence of tough cell walls and large central vacuole. It details preparative techniques for botanical specimens. Each of the nine chapters of this book covers the basic principles, useful applications, and reliable procedures used on the method of electron microscopy. Other topics discussed in each chapter include the general preparation and straining of thin sections, quantitative morphological analysis, and enzyme cytochemistry. This book also explains the immunogold labelling, rapid-freezing methods, and ambient- and low-temperature scanning electron microscopy among others. This book will be invaluable to general scientists, biologists, botanists, and students specializing in plant anatomy.

Electron Microscopy of Plant Cells

Electron Microscopy of Plant Cells PDF Author: C Hawes
Publisher: Academic Press
ISBN: 0323148980
Category : Science
Languages : en
Pages : 477

Book Description
Electron Microscopy of Plant Cells serves as manual or reference of major modern techniques used to prepare plant material for transmission and scanning electron microscopy. There have been other books that generally discuss electron microscope methodology. This book focuses on problem areas encountered through the presence of tough cell walls and large central vacuole. It details preparative techniques for botanical specimens. Each of the nine chapters of this book covers the basic principles, useful applications, and reliable procedures used on the method of electron microscopy. Other topics discussed in each chapter include the general preparation and straining of thin sections, quantitative morphological analysis, and enzyme cytochemistry. This book also explains the immunogold labelling, rapid-freezing methods, and ambient- and low-temperature scanning electron microscopy among others. This book will be invaluable to general scientists, biologists, botanists, and students specializing in plant anatomy.

Methods in Plant Electron Microscopy and Cytochemistry

Methods in Plant Electron Microscopy and Cytochemistry PDF Author: William V. Dashek
Publisher: Springer Science & Business Media
ISBN: 1592592325
Category : Science
Languages : en
Pages : 297

Book Description
Hands-on experimentalists describe the cutting-edge microscopical methods needed for the effective study of plant cell biology today. These powerful techniques, all described in great detail to ensure successful experimental results, range from light microscope cytochemistry, autoradiography, and immunocytochemistry, to recent developments in fluorescence, confocal, and dark-field microscopies. Important advances in both conventional and scanning electron microscopies are also fully developed, together with such state-of-the-art ancillary techniques as high-resolution autoradiography, immunoelectron microscopy, X-ray microanalysis, and electron systems imaging. Easy-to-use and up-to-date, Methods in Plant Electron Microscopy and Cytochemistry offers today's plant scientists a first class collection of readily reproducible light and electron microscopical methods that will prove the new standard for all working in the field.

Electron Microscopy and Cytochemistry of Plant Cells

Electron Microscopy and Cytochemistry of Plant Cells PDF Author: John Lloyd Hall
Publisher: Elsevier-North-Holland Biomedical Press
ISBN:
Category : Science
Languages : en
Pages : 464

Book Description


Biological Electron Microscopy

Biological Electron Microscopy PDF Author: Michael J. Dykstra
Publisher: Springer Science & Business Media
ISBN: 146840010X
Category : Science
Languages : en
Pages : 368

Book Description
In this practical text, the author covers the fundamentals of biological electron microscopy - including fixation, instrumentation, and darkroom work - to provide an excellent introduction to the subject for the advanced undergraduate or graduate student.

Electron Microscopy

Electron Microscopy PDF Author: John J. Bozzola
Publisher: Jones & Bartlett Learning
ISBN: 9780763701925
Category : Medical
Languages : en
Pages : 702

Book Description
New edition of an introductory reference that covers all of the important aspects of electron microscopy from a biological perspective, including theory of scanning and transmission; specimen preparation; darkroom, digital imaging, and image analysis; laboratory safety; interpretation of images; and an atlas of ultrastructure. Generously illustrated with bandw line drawings and photographs. Annotation copyrighted by Book News, Inc., Portland, OR

Molecular Biology of the Cell

Molecular Biology of the Cell PDF Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0

Book Description


Cryotechniques in Biological Electron Microscopy

Cryotechniques in Biological Electron Microscopy PDF Author: Rudolf A. Steinbrecht
Publisher: Springer Science & Business Media
ISBN: 3642728154
Category : Science
Languages : en
Pages : 308

Book Description
To preserve tissue by freezing is an ancient concept going back pre sumably to the practice of ice-age hunters. At first glance, it seems as simple as it is attractive: the dynamics of life are frozen in, nothing is added and nothing withdrawn except thermal energy. Thus, the result should be more life-like than after poisoning, tan ning and drying a living cell as we may rudely call the conventional preparation of specimens for electron microscopy. Countless mishaps, however, have taught electron microscopists that cryotechniques too are neither simple nor necessarily more life-like in their outcome. Not too long ago, experts in cryotechniques strictly denied that a cell could truly be vitrified, i.e. that all the solutes and macro molecules could be fixed within non-crystalline, glass-like solid water without the dramatic shifts and segregation effects caused by crystallization. We now know that vitrification is indeed pos sible. Growing insight into the fundamentals of the physics of water and ice, as well as increasing experience of how to cool cells rapidly enough have enlivened the interest in cryofixation and pro duced a wealth of successful applications.

Ontogeny, Cell Differentiation, and Structure of Vascular Plants

Ontogeny, Cell Differentiation, and Structure of Vascular Plants PDF Author: Roger Buvat
Publisher: Springer Science & Business Media
ISBN: 3642736351
Category : Science
Languages : en
Pages : 592

Book Description
With improved microscope and preparation techniques, studies of histo logical structures of plant organisms experienced a revival of interest at the end of the 19th century. From that time, histological data have sub stantially studies of the pioneers in botanical science. From the beginning of the 20th century, the microscope allowed research in cell structure, the general functional unit of living beings. Advances in cytology gradually influenced histology, at first, however, rather timidly. Only the new and spectacular progress in ultrastructural cytology and cytochemistry led to a great increase in modern work on the structures of vascular plants and the related ontogenical and physiological data, thanks to the use of the electron microscope and the contribution of molecular biology. Not only did new techniques lead to new approaches, but achieve ments in general biology shifted the orientation of research, linking in vestigation to the physiological aspects of cell and tissue differentiation. Among these, the demonstration of the general principles of develop ment, and the characterization of molecules common to plants and animals, which control and govern the main basic functions of cells and tissues, have widened the scope of modern research on plant struc tures. Present trends in biological research show that it is necessary to know the structures thoroughly, from the ultrastructural cytological scale to the scale of tissue and organ arrangement, even for physiological research on either cells, tissues, or whole organs. The study of growth factors, differ entiation, or organogenesis can be mentioned as an example.

Advances in Plant Ecophysiology Techniques

Advances in Plant Ecophysiology Techniques PDF Author: Adela M. Sánchez-Moreiras
Publisher: Springer
ISBN: 3319932330
Category : Science
Languages : en
Pages : 491

Book Description
This handbook covers the most commonly used techniques for measuring plant response to biotic and abiotic stressing factors, including: in vitro and in vivo bioassays; the study of root morphology, photosynthesis (pigment content, net photosynthesis, respiration, fluorescence and thermoluminiscence) and water status; thermal imaging; the measurement of oxidative stress markers; flow cytometry for measuring cell cycle and other physiological parameters; the use of microscope techniques for studying plant microtubules; programmed-cell-death; last-generation techniques (metabolomics, proteomics, SAR/QSAR); hybridization methods; isotope techniques for plant and soil studies; and the measurement of detoxification pathways, volatiles, soil microorganisms, and computational biology.

The Study of Plant Structure

The Study of Plant Structure PDF Author: Terence P. O'Brien
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 370

Book Description