Effects of Cyclic Stretch on Surfactant Secretion and Cell Viability in Alveolar Epithelial Cells Grown in Culture PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Effects of Cyclic Stretch on Surfactant Secretion and Cell Viability in Alveolar Epithelial Cells Grown in Culture PDF full book. Access full book title Effects of Cyclic Stretch on Surfactant Secretion and Cell Viability in Alveolar Epithelial Cells Grown in Culture by Stephen Paul Arold. Download full books in PDF and EPUB format.
Author: Stephen Paul Arold Publisher: ISBN: Category : Languages : en Pages : 258
Book Description
Abstract: Mechanical ventilation is vital among patients suffering from acute lung injury; however, studies have shown that it likely damages the lung through ventilator induced lung injury (VILI). VILI is thought to arise partly from the deleterious effects of non-physiologic forces imparted to the pulmonary epithelium. We have demonstrated that a novel ventilation strategy, "variable ventilation," whereby tidal volume is varied on a cycle-by-cycle basis, increases pulmonary surfactant content in rodents. We hypothesized that the variable stretch patterns enhanced surfactant secretion in the alveolar epithelial type II (AEII) cells. The broad goal of this study was to directly test this hypothesis. Here we describe the design and validation of a stretching device capable of delivering cyclic, biaxial strains to cells grown in culture including the novel stretch patterns required for this study. We utilized this device to characterize the effects of strain and underlying substratum on surfactant secretion and cell viability on the mouse lung epithelial-12 (MLE-12) cell line. Next, we cultured rat AEII cells and examined the combined effects of time duration and stretch magnitude on surfactant secretion and uptake, cell viability, and secretion of surfactant proteins. Finally, we stretched cultured AEII cells utilizing a "variable stretch" pattern mimicking those conditions thought to occur in-vivo during variable ventilation. We found that the MLE-12 cells did not exhibit surfactant secretion in response to stretch, however surfactant secretion was enhanced when grown on a fibronectin-like substratum in comparison with those grown on collagen I. While isolated AEII cells increased surfactant secretion in response to short periods of strain, 1 hour of cyclic stretch diminished surfactant secretion regardless of strain magnitude. Finally, variable stretch patterns applied to AEII increased surfactant secretion at high and moderate degrees of strain. This suggests ventilator strategies utilizing monotonous ventilation patterns may inhibit surfactant secretion in a clinical setting, whereas application of variable stretch may result in enhanced surfactant secretion into the alveolar space, possibly reducing VILI. These results may have a broader application to physiologic systems in general where purely periodic signals imposed by human intervention could have a detrimental effect on a system's natural function.
Author: Stephen Paul Arold Publisher: ISBN: Category : Languages : en Pages : 258
Book Description
Abstract: Mechanical ventilation is vital among patients suffering from acute lung injury; however, studies have shown that it likely damages the lung through ventilator induced lung injury (VILI). VILI is thought to arise partly from the deleterious effects of non-physiologic forces imparted to the pulmonary epithelium. We have demonstrated that a novel ventilation strategy, "variable ventilation," whereby tidal volume is varied on a cycle-by-cycle basis, increases pulmonary surfactant content in rodents. We hypothesized that the variable stretch patterns enhanced surfactant secretion in the alveolar epithelial type II (AEII) cells. The broad goal of this study was to directly test this hypothesis. Here we describe the design and validation of a stretching device capable of delivering cyclic, biaxial strains to cells grown in culture including the novel stretch patterns required for this study. We utilized this device to characterize the effects of strain and underlying substratum on surfactant secretion and cell viability on the mouse lung epithelial-12 (MLE-12) cell line. Next, we cultured rat AEII cells and examined the combined effects of time duration and stretch magnitude on surfactant secretion and uptake, cell viability, and secretion of surfactant proteins. Finally, we stretched cultured AEII cells utilizing a "variable stretch" pattern mimicking those conditions thought to occur in-vivo during variable ventilation. We found that the MLE-12 cells did not exhibit surfactant secretion in response to stretch, however surfactant secretion was enhanced when grown on a fibronectin-like substratum in comparison with those grown on collagen I. While isolated AEII cells increased surfactant secretion in response to short periods of strain, 1 hour of cyclic stretch diminished surfactant secretion regardless of strain magnitude. Finally, variable stretch patterns applied to AEII increased surfactant secretion at high and moderate degrees of strain. This suggests ventilator strategies utilizing monotonous ventilation patterns may inhibit surfactant secretion in a clinical setting, whereas application of variable stretch may result in enhanced surfactant secretion into the alveolar space, possibly reducing VILI. These results may have a broader application to physiologic systems in general where purely periodic signals imposed by human intervention could have a detrimental effect on a system's natural function.
Author: Jiro Nagatomi Publisher: CRC Press ISBN: 1420091212 Category : Medical Languages : en Pages : 566
Book Description
Mechanobiology—the study of the effects of mechanical environments on the biological processes of cells—has evolved from traditional biomechanics via the incorporation of strong elements of molecular and cell biology. Currently, a broad range of organ systems are being studied by surgeons, physicians, basic scientists, and engineers. These mechanobiologists aim to create new therapies and further biological understanding by quantifying the mechanical environment of cells and the molecular mechanisms of mechanically induced pathological conditions. To achieve these goals, investigators must be familiar with both the basic concepts of mechanics and the modern tools of cellular/molecular biology. Unfortunately, current literature contains numerous studies that misuse standard mechanical estimations and terminology, or fail to implement appropriate molecular analyses. Therefore, the Mechanobiology Handbook not only presents cutting-edge research findings across various fields and organ systems, but also provides the elementary chapters on mechanics and molecular analysis techniques to encourage cross-field understanding and appropriate planning. Aided by the continuous advancement of research tools in both mechanics and biology, more sophisticated experiments and analyses are possible—thus fueling the growth of the field of mechanobiology. Considering the complexity of the mechanics and the biology of the human body, most of the world of biomechanics remains to be studied. Since the field is still developing, the Mechanobiology Handbook does not force one unified theory, but brings out many different viewpoints and approaches to stimulate further research questions.
Author: Jiro Nagatomi Publisher: CRC Press ISBN: 042981674X Category : Medical Languages : en Pages : 705
Book Description
Mechanobiology—the study of the effects of mechanics on biological events—has evolved to answer numerous research questions. Mechanobiology Handbook 2nd Edition is a reference book for engineers, scientists, and clinicians who are interested in mechanobiology and a textbook for senior undergraduate to graduate level students of this growing field. Readers will gain a comprehensive review of recent research findings as well as elementary chapters on solid mechanics, fluid mechanics, and molecular analysis techniques. The new edition presents, in addition to the chapters of the first edition, homework problem sets that are available online and reviews of research in uncovered areas. Moreover, the new edition includes chapters on statistical analysis, design of experiments and optical imaging. The editors of this book are researchers and educators in mechanobiology. They realized a need for a single volume to assist course instructors as a guide for didactic teaching of mechanobiology to a diverse student body. A mechanobiology course is frequently made up of both undergraduate and graduate students pursuing degrees in engineering, biology, or integrated engineering and biology. Their goal was to present both the elementary and cutting-edge aspects of mechanobiology in a manner that is accessible to students from many different academic levels and from various disciplinary backgrounds. Moreover, it is their hope that the readers of Mechanobiology Handbook 2nd Edition will find study questions at the end of each chapter useful for long-term learning and further discussion. Comprehensive collection of reviews of recent research Introductory materials in mechanics, biology, and statistics Discussion of pioneering and emerging mechanobiology concepts Presentation of cutting-edge mechanobiology research findings across various fields and organ systems End of chapter study questions, available online Considering the complexity of the mechanics and the biology of the human body, most of the world of mechanobiology remains to be studied. Since the field is still developing, the Mechanobiology Handbook raises many different viewpoints and approaches with the intention of stimulating further research endeavours.
Author: Kwang W. Jeon Publisher: Gulf Professional Publishing ISBN: 9780123646491 Category : Science Languages : en Pages : 364
Book Description
International Review of Cytology presents current advances and comprehensive reviews in cell biology - both plant and animal. Authored by some of the foremost scientists in the field, each volume provides up-to-date information and directions for future research. Articles in this volume address Calpain proteases in cell adhesion and motility; Transforming growth factor beta (TGF-b) and programmed cell death in the vertebrate retina; Molecular Mechanism of Apoptosis Induced by Mechanical Forces; Cellular functions of ER chaperones Calreticulin, calnexin, and Erp57; Plasticity of nonapeptidergic neurosecretory cells in connection with the discovery of neurosecretion; Interactions between virus proteins and host cell membranes during viral life cycle; Nerve ending "signal" proteins GAP-43, MARCKS and BASP1.
Author: Bengt Robertson Publisher: Elsevier Publishing Company ISBN: Category : Medical Languages : en Pages : 800
Book Description
There has been a spectacular development in both basic research on the pulmonary surfactant system and the clinical use of surfactant for treatment of lung diseases, especially in the neonatal period. In particular, the molecular biology and physiological roles of the surfactant-associated proteins (SP-A) have been clarified during the last few years; the structure and functions of these proteins therefore constitute an important hot topic, and are reviewed in several chapters of this volume. including surface chemistry and ultrastructure of surfactant components, the role of surfactant in lung mechanics and alveolar liquid homeostasis, and surfactant metabolism and its hormonal control. However, as mentioned above, clinical applications of surfactant research have become increasingly important and are therefore dealt with extensively in the remaining chapters. during the last few years to illustrate the fascinating complexity of the surfactant system. Hence, the main mission of this volume is to catalyze the future development in this field with the exchange of ideas between scientific workers from different disciplines.
Author: Claude Gaultier Publisher: Springer ISBN: 1461475376 Category : Medical Languages : en Pages : 464
Book Description
Knowledge about the mechanisms of lung development has been growing rapidly, especially with regard to cellular and molecular aspects of growth and differentiation. This authoritative international volume reviews key aspects of lung development in health and disease by providing a comprehensive review of the complex series of cellular and molecular interactions required for lung development. It covers such topics as pulmonary hypoplasia, effects of malnutrition, and pulmaonary angiogenesis. An indispensable reference for all those involved in studying or treating lung disease in neonates and children, the book offers a unique view of the development of this essential organ.
Author: Didier Dreyfuss Publisher: CRC Press ISBN: 1420019260 Category : Medical Languages : en Pages : 776
Book Description
This reference surveys current best practices in the prevention and management of ventilator-induced lung injury (VILI) and spans the many pathways and mechanisms of VILI including cell injury and repair, the modulation of alveolar-capillary barrier properties, and lung and systemic inflammatory consequences of injurous mechanical ventilation. Cons
Author: Venkataramana K Sidhaye Publisher: Academic Press ISBN: 0128038810 Category : Medical Languages : en Pages : 277
Book Description
Lung Epithelial Biology in the Pathogenesis of Pulmonary Disease provides a one-stop resource capturing developments in lung epithelial biology related to basic physiology, pathophysiology, and links to human disease. The book provides access to knowledge of molecular and cellular aspects of lung homeostasis and repair, including the molecular basis of lung epithelial intercellular communication and lung epithelial channels and transporters. Also included is coverage of lung epithelial biology as it relates to fluid balance, basic ion/fluid molecular processes, and human disease. Useful to physician and clinical scientists, the contents of this book compile the important and most current findings about the role of epithelial cells in lung disease. Medical and graduate students, postdoctoral and clinical fellows, as well as clinicians interested in the mechanistic basis for lung disease will benefit from the books examination of principles of lung epithelium functions in physiological condition. Provides a single source of information on lung epithelial junctions and transporters Discusses of the role of the epithelium in lung homeostasis and disease Includes capsule summaries of main conclusions as well as highlights of future directions in the field Covers the mechanistic basis for lung disease for a range of audiences
Author: Stephen Paul Arold
Author: Stephen Paul Arold
Author: 
Author: Jiro Nagatomi
Author: Jiro Nagatomi
Author: Kwang W. Jeon
Author: 
Author: Bengt Robertson
Author: Claude Gaultier
Author: Didier Dreyfuss
Author: Venkataramana K Sidhaye