Dna Nanotechnology For Bioanalysis: From Hybrid Dna Nanostructures To Functional Devices PDF Download

Author: Giuseppe Domenico Arrabito
Publisher: World Scientific
ISBN: 1786343819
Category : Science
Languages : en
Pages : 216

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Book Description
This book is intended for non-specialists and students, presenting a unique introduction to the field of DNA nanotechnology. The primary focus is on the extraordinary advantages of specificity and sensitivity obtained by integrating DNA nanostructures in bioanalytical devices.DNA Nanotechnology for Bioanalysis provides a concise and rigorous description for the fabrication of various types of functional nanostructures by optimized software-aided high-yield synthesis. Following this is the explanation of methods to decorate these nanostructures with molecules such as proteins, metal nanoparticles or bioorganic moieties covalently bonded onto DNA via self-assemblage processes. Also provided is a concise review on non-canonical DNA structures (such as G-quadruplexes) and their targeting by small molecules for applications in pharmacology. Finally, it describes the exciting applications of DNA nanostructures in life sciences and nanomedicine, including ultraspecific molecular delivery, control of cell behavior, analysis of cell lysate and DNA-based nano-tools for super-resolution sub-cellular imaging.

Author: Zhou Nie
Publisher: John Wiley & Sons
ISBN: 352784080X
Category : Science
Languages : en
Pages : 549

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Book Description
DNA Nanotechnology for Cell Research Comprehensive coverage of DNA nanotechnology with a focus on its biomedical applications in disease diagnosis, gene therapy, and drug delivery Bringing together multidisciplinary aspects of chemical, material, and biological engineering, DNA Nanotechnology for Cell Research: From Bioanalysis to Biomedicine presents an overview of DNA nanotechnology with emphasis on a variety of different applications in cell research and engineering, covering a unique collection of DNA nanotechnology for fundamental research and engineering of living cells, mostly in cellulo and in vivo, for the first time. Broad coverage of this book ranges from pioneering concepts of DNA nanotechnology to cutting-edge reports regarding the use of DNA nanotechnology for fundamental cell science and related biomedical engineering applications in sensing, bioimaging, cell manipulation, gene therapy, and drug delivery. The text is divided into four parts. Part I surveys the progress of functional DNA nanotechnology tools for cellular recognition. Part II illustrates the use of DNA-based biochemical sensors to monitor and image intracellular molecules and processes. Part III examines the use of DNA to regulate biological functions of individual cells. Part IV elucidates the use of DNA nanotechnology for cell-targeted medical applications. Sample topics covered in DNA Nanotechnology for Cell Research include: Selections and applications of functional nucleic acid toolkits, including DNA/RNA aptamers, DNAzymes, and riboswitches, for cellular recognition, metabolite detection, and liquid biopsy. Developing intelligent DNA nanodevices implemented in living cells for amplified cell imaging, smart intracellular sensing, and in cellulo programmable biocomputing. Harnessing dynamic DNA nanotechnology for non-genetic cell membrane engineering, receptor signaling reprogramming, and cellular behavior regulation. Construction of biocompatible nucleic acid nanostructures as precisely controlled vehicles for drug delivery, immunotherapy, and tissue engineering. Providing an up-to-date tutorial style overview along with a highly valuable in-depth perspective, DNA Nanotechnology for Cell Research is an essential resource for the entire DNA-based nanotechnology community, including analytical chemists, biochemists, materials scientists, and bioengineers.

Author: Chunhai Fan
Publisher: Springer Nature
ISBN: 3030548066
Category : Technology & Engineering
Languages : en
Pages : 406

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Book Description
The series Topics in Current Chemistry Collections presents critical reviews from the journal Topics in Current Chemistry organized in topical volumes. The scope of coverage is all areas of chemical science including the interfaces with related disciplines such as biology, medicine and materials science. The goal of each thematic volume is to give the non-specialist reader, whether in academia or industry, a comprehensive insight into an area where new research is emerging which is of interest to a larger scientific audience. Each review within the volume critically surveys one aspect of that topic and places it within the context of the volume as a whole. The most significant developments of the last 5 to 10 years are presented using selected examples to illustrate the principles discussed. The coverage is not intended to be an exhaustive summary of the field or include large quantities of data, but should rather be conceptual, concentrating on the methodological thinking that will allow the non-specialist reader to understand the information presented. Contributions also offer an outlook on potential future developments in the field. The chapter "DNA-Programmed Chemical Synthesis of Polymers and Inorganic Nanomaterials" is available open access under a CC BY 4.0 License via link.springer.com.

Author: Eugen Stulz
Publisher: John Wiley & Sons
ISBN: 111869693X
Category : Technology & Engineering
Languages : en
Pages : 538

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Book Description
This book covers the emerging topic of DNA nanotechnology and DNA supramolecular chemistry in its broader sense. By taking DNA out of its biological role, this biomolecule has become a very versatile building block in materials chemistry, supramolecular chemistry and bio-nanotechnology. Many novel structures have been realized in the past decade, which are now being used to create molecular machines, drug delivery systems, diagnosis platforms or potential electronic devices. The book combines many aspects of DNA nanotechnology, including formation of functional structures based on covalent and non-covalent systems, DNA origami, DNA based switches, DNA machines, and alternative structures and templates. This broad coverage is very appealing since it combines both the synthesis of modified DNA as well as designer concepts to successfully plan and make DNA nanostructures. Contributing authors have provided first a general introduction for the non-specialist reader, followed by a more in-depth analysis and presentation of their topic. In this way the book is attractive and useful for both the non-specialist who would like to have an overview of the topic, as well as the specialist reader who requires more information and inspiration to foster their own research.

Author: Thomas Edwardson
Publisher:
ISBN:
Category :
Languages : en
Pages :

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Book Description
"DNA is used for the storage and propagation of genetic information in all living organisms. It is the programmability and molecular recognition properties of DNA which make this possible. Taking advantage of these properties, researchers have developed DNA self-assembly as a highly predictable method of bottom-up nanofabrication. The resulting nanostructures have the potential to solve important problems in diverse fields, from nanophotonics to gene therapy. To date most DNA nanostructures have been made purely from unmodified, natural DNA. To achieve structural complexity needed for functional devices, researchers have created intricate designs which often require a large number of DNA strands of different sequence,with increased cost and assembly error rates. This thesis examines the chemical modification of nucleic acids and their integration into DNA nanostructures. This approach is expected to simplify design and significantly reduce the number of DNA components, while adding functional complexity. Specifically, the introduction of orthogonal supramolecular interactions to DNA nanostructures and the resultant properties of these hybrid systems is investigated. Firstly, the synthesis of novel dendritic DNA amphiphiles and their self-assembly properties are investigated. The site-specific positioning of the amphiphiles on a cubic DNA scaffold allows the anisotropic organization of hydrophobic 'residues', in a manner similar to the side-chains on a protein backbone. A new set of self-assembly rules is discovered, in which these nanostructures show a geometry-dependent inter- or intra-molecular association. This is used to create the first example of a DNA nanostructure which can encapsulate and release small molecule drugs, an important challenge in nanomedicine. Secondly, this synthetic methodology is developed to produce a family of DNA-polymer conjugates, with sequence-defined polymers appended to DNA. These conjugates exhibit tunable self-assembly properties, dependent on the sequence of monomers in the polymer portion. Finally, the modular synthetic approaches developed are used to produce novel gold-binding DNA conjugates. The organization of these on prismatic DNA scaffolds creates patterns of DNA strands which can be efficiently transferred to gold nanoparticles. This template-guided 'printing' approach provides control over the number, directionality, geometry and sequence asymmetry of DNA strands bound to gold nanoparticle. Overall, the development of new synthetically modified oligonucleotides and their incorporation into nanostructures is shown to augment the field of DNA nanotechnology, introducing protein-inspired interactions and providing new tools for the creation of functional nanodevices for applications in the fields of nanophotonics, nanoelectronics and biological sensing, as well as drug and oligonucleotide delivery." --

Author: Thimmaiah Govindaraju
Publisher: CRC Press
ISBN: 0429767676
Category : Science
Languages : en
Pages : 426

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Book Description
Nucleic acids have structurally evolved over billions of years to effectively store and transfer genetic information. In the 1980s, Nadrian Seeman’s idea of constructing a 3D lattice from DNA led to utilizing DNA as nanomolecular building blocks to create emergent molecular systems and nanomaterial objects. This bottom-up approach to construct nanoscale architectures with DNA marked the beginning of a new field, DNA nanotechnology, contributing significantly to the broad area of nanoscience and nanotechnology. The molecular architectonics of small "designer" molecules and short DNA sequences through complementary binding interaction engenders well-defined functional nanoarchitectures with realistic applications in areas ranging from biology to materials science and is termed "DNA nanoarchitectonics." This book discusses novel approaches adapted by leading researchers from all over the world to create functional nucleic acid molecular systems and nanoarchitectures. Individual chapters contributed by active practitioners provide fundamental and advanced knowledge emanated from their own and others’ work. Each chapter includes numerous illustrations, historical perspectives, case studies and practical examples, critical discussions, and future prospects. This book can serve as a practical handbook or as a textbook for advanced undergraduate- and graduate-level students of nanotechnology and DNA nanotechnology, supramolecular chemistry, and nanoarchitectonics and researchers working on macromolecular science, nanotechnology, chemistry, biology, and medicine, especially those with an interest in sensors, biosensors, nanoswitches and nanodevices, diagnostics, drug delivery, and therapeutics.

Author: Jørgen Kjems
Publisher: Springer Science & Business Media
ISBN: 3642388159
Category : Science
Languages : en
Pages : 340

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Book Description
This volume on nucleic acid nanotechnology offers authoritative, up-to-date and comprehensive coverage of nanotechnological studies and applications of nucleic acids. It provides reviews of various aspects of nucleic acid nanotechnology, each written by an internationally leading expert in the field, and presents state-of-the-art and recent advances in nucleic acid synthetic modifications, nanoscale design, manipulation and current and future applications in bioengineering, medicine, electronics, genetic analysis, chemistry, molecular biology, surface and material sciences. It examines how nucleic acid research is merging with nanotechnology, allowing the nanoscale properties of nucleic acid to be exploited in performing challenging nanotechnological tasks, from nanorobotics and nanosensing to nucleic acid computing. This book will above all benefit anyone who is interested in nanotechnological concepts of nucleic acid design and applications, and offers a valuable resource for teaching these concepts. It is essential reading for a broad audience of scientists both in academia and industry who wish to expand their expertise on the potential of nucleic acid functions and applications.

Author: Nataša Jonoska
Publisher: Springer Nature
ISBN: 9811998914
Category : Computers
Languages : en
Pages : 442

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Book Description
This open access book provides a unique and state-of-the-art view on DNA nanotechnology with an eye toward future developments. Intended as a tribute to Nadrian C. Seeman, who founded the field of DNA nanotechnology, the content is an exciting mixture of technical and non-technical material, reviews, tutorials, perspectives, new findings, and open questions. The book aims to inspire current researchers to sit back and think about the big picture, while also enticing new researchers to enter the field. Most of all, the book captures voices from a unique moment in time: 40 years after the publication of the first paper that envisioned DNA nanotechnology. From this vantage point, what are the untold stories, the unspoken concerns, the underlying fundamental issues, the overlooked opportunities, and the unifying grand challenges? What will help us see more clearly, see more creatively, or see farther? What is transpiring right now that could pave the way for the future? To address these questions, leading researchers have contributed 22 chapters, grouped into five sections: perspectives, chemistry and physics, structures, biochemical circuits, and spatial systems. This book will be an important reference point in the field of DNA nanotechnology, both for established researchers looking to take stock of the field and its future, and for newcomers such as graduate students and researchers in other fields who are beginning to appreciate the power and applicability of its methods.

Author: Joshua B. Edel
Publisher: William Andrew
ISBN: 143773474X
Category : Science
Languages : en
Pages : 189

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Book Description
Engineered Nanopores for Bioanalytical Applications is the first book to focus primarily on practical analytical applications of nanopore development. These nanoscale analytical techniques have exciting potential because they can be used in applications such as DNA sequencing, DNA fragment sizing, DNA/protein binding, and protein/protein binding. This book provides a solid professional reference on nanopores for readers in academia, industry and engineering and biomedical fields. In addition, the book describes the instrumentation, fabrication, and experimental methods necessary to carry out nanopore-based experiments for developing new devices. Includes application case studies for detection, identification and analysis of biomolecules and related functional nanomaterials Introduces the techniques of manufacturing solid state materials with functional nanopores Explains the use of nanopores in DNA sequencing and the wider range of applications from environmental monitoring to forensics

Author: Sandra J Rosenthal
Publisher: Springer Science & Business Media
ISBN: 159259901X
Category : Science
Languages : en
Pages : 241

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Book Description
Hands-on experts in nanomaterial synthesis and application describe in detail the key experimental techniques currently employed in novel materials synthesis, dynamic cellular imaging, and biological assays. The author's emphasize diverse strategies to synthesize and functionalize the use of nanoparticles for biological applications. Additional chapters focus on the use of biological components (peptides, antibodies, and DNA) to synthesize and organize nanoparticles to be used a building block in larger assemblies. These new materials make it possible to image cellular processes for longer durations, leading to high throughput cellular-based screens for drug discovery, drug delivery, and diagnostic applications. Highlights include overview chapters on quantum dots and DNA nanotechnology, and cutting-edge techniques in the emerging nanobiotachnology arena.