Entomopathogenic Nematodes and Their Symbiotic Bacteria PDF Download

Author: S. Sivaramakrishnan
Publisher: Springer
ISBN: 9781071614440
Category : Science
Languages : en
Pages : 176

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Book Description
This book provides basic information and different protocols associated with the Entomopathogenic nematodes (EPNs) and their symbiotic bacteria. Entomopathogenic nematodes (EPNs) of the genera Steinernema and Heterorhabditis and their associated bacterial symbionts Xenorhabdus and Photorhabdus aid nematode infective juveniles (IJs) in infecting and killing their insect hosts, creating a unique tripartite complex of host-vector-symbiont interactions. Due to this insect killing capability, EPNs are used as biological control agents of economically important insect pests. They are also a model system to study host-parasite interactions. It provides a systematic approach to various nematode procedures including pathogenicity, reproduction, foraging behaviour. It gives a brief outline on historical aspects, nematode-bacterium complex, biology and chemical ecology of EPNs. It concisely describes host insect rearing, nematode sampling and storage, isolation techniques, counting, handling and staining of nematodes, characterization including morphological, molecular and ecological studies, mass production, virulence bioassay, field application and efficacy. The book also includes methods and techniques for their associated symbiotic bacteria. This book serves as a laboratory manual and assists the readers to undertake advanced research in different aspects related to nematodes. It is useful for researchers in the fields of nematology, microbiology, bacteriology and entomology.

Author:
Publisher: BRILL
ISBN: 9004285342
Category : Science
Languages : en
Pages : 454

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Book Description
In Advances in Entomopathogenic Nematode Taxonomy and Phylogeny the numerous species of Steinernema and Heterorhabditis described since the previous volume, published in 2007, are evaluated and discussed. Valid species proposed from 2007-2015 are covered in detail, each taxon having an illustrated diagnostic description and additional data on molecular characterisation, distribution and biology, etc. An addendum gives brief details of species proposed during 2016. An overview of the taxonomy of the two families provides an up-to-date list of species for both genera, including new synonyms and detailed commentary on specific status where appropriate. Tabular keys to all valid species of Steinernema and Heterorhabditis assist in diagnostics. A chapter on phylogeny and phylogeography completes the book.

Author: Randy Gaugler
Publisher: CABI
ISBN: 9780851997919
Category : Science
Languages : en
Pages : 410

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Book Description
Nematodes that are parasites of insects are no longer a laboratory curiosity. They have begun to be accepted as environmentally benign alternatives to the use of chemical insecticides, for the control of insect pests. Nematode worms are now applied as biological control agents against insectpests of numerous horticultural and agricultural crops. This book provides a comprehensive review of entomopathogenic nematology. It begins by reviewing fundamental biology and setting a taxonomic foundation for nematodes and their bacterial symbionts. Several chapters are devoted to functionalprocesses involved in parasitism and to nematode ecology. Later chapters describe technological advances and control methodologies.

Author: Randy Gaugler
Publisher: CRC Press
ISBN: 1351088645
Category : Science
Languages : en
Pages : 415

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Book Description
The editors of this book, who are world renown for their creativity with entomopathogenic nematodes, have assembled the foremost authorities from four continents to contribute on basic and applied concepts. The authors have taken advantage of this opportunity to express their views to a wide scientific audience. They have combined their international experience so that the latest developments in this fascinating and rapidly expanding field are present in comprehensive manner with diverse topics ranging from biological control theory to organismal and molecular biology.

Author: Raquel Campos-Herrera
Publisher: Springer
ISBN: 3319182668
Category : Technology & Engineering
Languages : en
Pages : 532

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Book Description
Achieving a sustainable agriculture requires integrating advances in multiples disciplines, covering both fundamental and applied research in a common objective: enhancing crop health for better productions. This first volume of the Series “Sustainability in plant and crop protection” presents a comprehensive and multi-disciplinary compendium about the recent achievements in the use of entomopathogenic nematodes (EPNs) as biological control in a global scale. The volume is organized in a first section discussing the last discoveries on the biology and ecology of the EPN, a second section covering the advances on the EPN productions and release, and a third section with multiples case-studies in which the concepts and ideas on the two previous sections are integrated and discussed. An essential tool for researchers and professionals working to advance in the sustainable use of our resources.

Author: George O. Poinar Jr.
Publisher: BRILL
ISBN: 9047428668
Category : Science
Languages : en
Pages : 436

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Book Description
Nematodes are one of the most abundant groups of invertebrates on the face of the earth. Their numbers are estimated to range from 1000 per cm2 in the sand-covered hydrogen sulphide ‘black zone’ beneath the ocean floors to 1.2 billion in a single hectare of soil. Estimates for their species diversity range from 100 000 to 10 million. The past history of nematodes is a mystery, since very few fossils have been discovered. This book establishes a solid base in palaeonematology with descriptions of 66 new fossil species and accounts of all previous fossil and subfossil nematodes from sedimentary deposits, coprolites, amber and mummies. It shows how nematode fossils can be used to establish lineages at various locations and time periods in the earth’s history and when nematodes entered into symbiotic and parasitic associations with plants and animals.

Author: Khuong Nguyen
Publisher: BRILL
ISBN: 9047422392
Category : Science
Languages : en
Pages : 832

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Book Description
The volume deals with morphology, taxonomy and systematics of entomopathogenic nematodes (EPN) in the families Steinernematidae and Heterorhabditidae and bacteria associated with these nematodes. In the first part, history, taxonomic status, family and genus definitions of EPN are mentioned. In the second part, all useful aspects of morphology and methodology of EPN are mentioned with illustrations and SEM photographs and instructions on how to make the measurements, and how to use them in taxonomic work. A polychotomous key with SEM photographs and illustrations is provided. In the third part, full descriptions of all species are presented with latest information about each species. In the fourth part, molecular and phylogenetic methods for working with EPN are presented, including the latest information and instructions on how to use molecular data in taxonomic work. In the last part, bacteria associated with this group of nematodes are discussed in the context of the latest information about methodology, biology and taxon.

Author: Martin Dworkin
Publisher: Springer
ISBN: 9780387334882
Category : Science
Languages : en
Pages : 959

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Book Description
With the launch of its first electronic edition, The Prokaryotes, the definitive reference on the biology of bacteria, enters an exciting new era of information delivery. Subscription-based access is available. The electronic version begins with an online implementation of the content found in the printed reference work, The Prokaryotes, Second Edition. The content is being fully updated over a five-year period until the work is completely revised. Thereafter, material will be continuously added to reflect developments in bacteriology. This online version features information retrieval functions and multimedia components.

Author: Lawrence A. Lacey
Publisher: Academic Press
ISBN: 0123869005
Category : Science
Languages : en
Pages : 513

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Book Description
Manual of Techniques in Invertebrate Pathology, Second Edition, describes a wide range of techniques used in the identification, isolation, propagation/cultivation, bioassay, quantification, preservation, and storage of the major groups of entomopathogens, including entomophthorales, entomopathogenic fungi, entomopathogenic bacteria of the Bacilli, Nematode parasites, and pathogens and parasites of terrestrial molluscs. The book presents the perspectives of an international group of experts in the fields of invertebrate pathology, including microbiology, mycology, virology, nematology, biological control, and integrated pest management. Organized into 15 chapters, the book covers methods for the study of virtually every major group of entomopathogen, as well as methods for discovery and diagnosis of entomopathogens and the use of complementary methods for microscopy. It discusses the use of molecular techniques for identifying and determining phylogeny, factors that contribute to resistance to entomopathogens, and several other aspects of the science of invertebrate pathology. It also explains initial handling and diagnosis of diseased invertebrates, basic techniques in insect virology, and bioassay of bacterial entomopathogens against insect larvae. In addition, the reader is introduced to the use of bacteria against soil-inhabiting insects and preservation of entomopathogenic fungal cultures. The remaining chapters focus on research methods for entomopathogenic microsporidia and other protists, how the pathogenicity and infectivity of entomopathogens to mammals are tested, and preparations of entomopathogens and diseased specimens for more detailed study using microscopy. Experienced insect pathologists, biologists, entomologists, students, biotechnology personnel, technicians, those working in the biopesticide industry, and government regulators will find this manual extremely helpful. - Step-by-step instructions for the latest techniques on how to isolate, identify, culture, bioassay and store the major groups of entomopathogens - New edition fully updated to address changes in the taxonomy of the vast majority of taxa - Discussion of safety testing of entomopathogens in mammals and also broader methods such as microscopy and molecular techniques - Provides extensive supplemental literature and recipes for media, fixatives and stains

Author: Md. Aslam Khan
Publisher: Springer Nature
ISBN: 3030230457
Category : Science
Languages : en
Pages : 398

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Book Description
This Volume comprises 14 chapters in an attempt to provide the reader with available information on safe and effective use of entomopathogens. Chapters in this book dealing with soil-borne entomopathogens and their phylogeny also provide a review on most updated information of their isolation and molecular identification. Employing fungal pathogens in biological control programmes plays a key role, and conidial thermotolerance and oxidative stress are examined in separate chapters. Entomopathogenic bacteria are able to kill their hosts quickly. An important contribution concerns informations provided upon bacterial cytotoxic factors on insect haemocytes. Nematodes are biological control agents safe to the environment. The information with respect to their direct and indirect effects on non-target organisms is provided. Viruses as highly specific, virulent candidates for use as biological insecticides are safe to non-target species. A separate chapter on the role of granuloviruses in IPM contributes a wealth of information. Biopesticides in combination with synthetic insecticides are reported as effective, economic, and eco-friendly. Understanding their interactions will certainly promote their uses. Finally, emphasis has been given on reviewing synergistic and antagonistic interactions of microbial and chemical pesticides, in other chapters.