DEVELOPPEMENTS DE LA SPECTROSCOPIE RMN EN BIOLOGIE STRUCTURALE ET METABOLIQUE PDF Download

Author: ADELINE.. EVRARD
Publisher:
ISBN:
Category :
Languages : fr
Pages : 170

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Book Description
CE TRAVAIL SE DIVISE EN DEUX PARTIES DISTINCTES, RMN STRUCTURALE A UNE DIMENSION ET RMN METABOLIQUE A UNE ET DEUX DIMENSIONS. DANS UNE PREMIERE PARTIE, UNE ANALYSE QUANTITATIVE DE SPECTRES PHOSPHORES DE LA PROTEINE SIR-FP60 NATIVE ET DANS DES ETATS REDOX CONNUS NOUS A PERMIS D'EBAUCHER UNE STRUCTURE DU SITE ACTIF DE LA NADP SULFITE REDUCTASE (SIR). DANS UNE DEUXIEME PARTIE, LA QUANTIFICATION DES SPECTRES D'EXTRAITS ACIDES CELLULAIRES DE POINTES DE RACINES DE MAIS EXCISEES ET L'ANALYSE QUANTITATIVE D'EXPERIENCES IN-VIVO NOUS ONT PERMIS D'ETUDIER LE SIGNAL-SUCRE ET LA TRANSITION NORMOXIE-ANOXIE CHEZ LES CELLULES VEGETALES. NOUS AVONS CONFIRME LE ROLE ACTIF DES HEXOKINASES DANS LA REPONSE ADAPTATIVE DES POINTES DE RACINES DE MAIS AU MANQUE DE SUBSTRATS CARBONES EXOGENES. NOUS AVONS EGALEMENT DECRIT UN SYSTEME EXPERIMENTAL POUR L'UTILISATION D'UN TUBE RMN DE DIAMETRE 5 MM. CE SYSTEME NOUS A PERMIS D'ISOLER COMPLETEMENT LES RESONANCES DU LACTATE, DE L'ALANINE ET DES LIPIDES QUI ONT DES FREQUENCES DE RESONANCE PROTON TROP PROCHES A UNE DIMENSION POUR ETRE TOTALEMENT SEPAREES. LES MILIEUX INTERNE ET EXTERNE ONT ETE ANALYSES PAR SPECTROSCOPIE PROTON A UNE DIMENSION POUR SUIVRE LA TRANSITION BRUTALE VERS L'ANOXIE. LES EXPERIENCES RMN A DEUX DIMENSIONS SE SONT REVELEES INDISPENSABLES POUR L'IDENTIFICATION DE METABOLITES DANS DES EXTRAITS ACELLULAIRES (CAS DU GLUCOSE-1-PHOSPHATE OU DU LACTATE PAR EXEMPLE). DANS UNE TROISIEME PARTIE, LES PROPRIETES DES SEQUENCES 2D UTILISABLES IN-VIVO ONT ETE ANALYSEES ET, NOUS AVONS MONTRE L'INTERET DE LA SEQUENCE COLOC POUR LA MESURE DE LA VARIATION DE QUANTITE DE GLUCOSE-1-PHOSPHATE, IMPOSSIBLE PAR RMN A UNE DIMENSION.

Author: Patrick Bertrand
Publisher: Springer Nature
ISBN: 3030396630
Category : Science
Languages : en
Pages : 420

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Book Description
Although originally invented and employed by physicists, electron paramagnetic resonance (EPR) spectroscopy has proven to be a very efficient technique for studying a wide range of phenomena in many fields, such as chemistry, biochemistry, geology, archaeology, medicine, biotechnology, and environmental sciences. Acknowledging that not all studies require the same level of understanding of this technique, this book thus provides a practical treatise clearly oriented toward applications, which should be useful to students and researchers of various levels and disciplines. In this book, the principles of continuous wave EPR spectroscopy are progressively, but rigorously, introduced, with emphasis on interpretation of the collected spectra. Each chapter is followed by a section highlighting important points for applications, together with exercises solved at the end of the book. A glossary defines the main terms used in the book, and particular topics, whose knowledge is not required for understanding the main text, are developed in appendices for more inquisitive readers.

Author: Gilles Gasser
Publisher: John Wiley & Sons
ISBN: 111851002X
Category : Science
Languages : en
Pages : 437

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Book Description
Understanding, identifying and influencing the biological systems are the primary objectives of chemical biology. From this perspective, metal complexes have always been of great assistance to chemical biologists, for example, in structural identification and purification of essential biomolecules, for visualizing cellular organelles or to inhibit specific enzymes. This inorganic side of chemical biology, which continues to receive considerable attention, is referred to as inorganic chemical biology. Inorganic Chemical Biology: Principles, Techniques and Applications provides a comprehensive overview of the current and emerging role of metal complexes in chemical biology. Throughout all of the chapters there is a strong emphasis on fundamental theoretical chemistry and experiments that have been carried out in living cells or organisms. Outlooks for the future applications of metal complexes in chemical biology are also discussed. Topics covered include: • Metal complexes as tools for structural biology • IMAC, AAS, XRF and MS as detection techniques for metals in chemical biology • Cell and organism imaging and probing DNA using metal and metal carbonyl complexes • Detection of metal ions, anions and small molecules using metal complexes • Photo-release of metal ions in living cells • Metal complexes as enzyme inhibitors and catalysts in living cells Written by a team of international experts, Inorganic Chemical Biology: Principles, Techniques and Applications is a must-have for bioinorganic, bioorganometallic and medicinal chemists as well as chemical biologists working in both academia and industry.

Author: Ursula Jakob
Publisher: Springer Science & Business Media
ISBN: 9400757875
Category : Science
Languages : en
Pages : 488

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Book Description
Many physiological conditions such as host defense or aging and pathological conditions such as neurodegenerative diseases, and diabetes are associated with the accumulation of high levels of reactive oxygen species and reactive nitrogen species. This generates a condition called oxidative stress. Low levels of reactive oxygen species, however, which are continuously produced during aerobic metabolism, function as important signaling molecules, setting the metabolic pace of cells and regulating processes ranging from gene expression to apoptosis. For this book we would like to recruit the experts in the field of redox chemistry, bioinformatics and proteomics, redox signaling and oxidative stress biology to discuss how organisms achieve the appropriate redox balance, the mechanisms that lead to oxidative stress conditions and the physiological consequences that contribute to aging and disease.

Author:
Publisher: Academic Press
ISBN: 0128172258
Category : Medical
Languages : en
Pages : 254

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Book Description
Mitochondrial Neuropathies, Volume 146, brings together experts in a range of diseases that damage the nervous system to present the role of mitochondrial dysfunction in their particular field, with this new release focusing on Mitochondrial dysfunction in Alzheimer's Disease, HIV and the mitochondrial: immune interface in the CNS, The Impact of mitochondrial damage in HIV-induced peripheral neuropathy, Mitochondrial dysfunction and the pathogenesis of chemotherapy-induced peripheral neuropathy, Disorders of mitochondrial biogenesis in peripheral neuropathy, Mitochondrial dysfunction and the pathogenesis of diabetic neuropathy, Manipulating mitochondria to treat peripheral neuropathy, and DCA therapy - the yin and yang of mitochondrial activation. - Contains contributions from a diverse group of experts - Presents a timely resource that brings together different fields to highlight a common pathogenic mechanism - Focuses on the role of mitochondria in diverse neuropathies

Author: Francis Rouessac
Publisher: John Wiley & Sons
ISBN: 1118681878
Category : Science
Languages : en
Pages : 494

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Book Description
Completely revised and updated, Chemical Analysis: Second Edition is an essential introduction to a wide range of analytical techniques and instruments. Assuming little in the way of prior knowledge, this text carefully guides the reader through the more widely used and important techniques, whilst avoiding excessive technical detail. Provides a thorough introduction to a wide range of the most important and widely used instrumental techniques Maintains a careful balance between depth and breadth of coverage Includes examples, problems and their solutions Includes coverage of latest developments including supercritical fluid chromatography and capillary electrophoresis

Author:
Publisher: Elsevier
ISBN: 012408107X
Category : Science
Languages : en
Pages : 593

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Book Description
Advances in Botanical Research publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences. The series features several reviews by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology and ecology. This thematic volume, number 71, features reviews on sea plants. Its chapters cover topics such as the role of algae in sustainability; the status of kelp exploitation and marine agronomy; potential applications for enzymatic recovery of metabolites from seaweeds; and many more. - Publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences - Features a wide range of reviews by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology, and ecology - Volume features reviews on sea plants

Author: Hervé Emonard
Publisher: Frontiers Media SA
ISBN: 2889450244
Category :
Languages : en
Pages : 131

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Book Description
The invasive character of a primary cancer is greatly dependent on numerous interactions between tumor cells and their extracellular surroundings. Matricellular receptors are defined as (cell-surface) receptors that bind extracellular matrix (ECM) structural proteins and soluble factors dynamically acting on ECM homeostasis. Matricellular receptors mediate numerous signalings from the extracellular environment to cell nucleus and drive main biological functions that are cell growth, survival and migration. Numerous data from the last decade evidence that matricellular receptors are biosensors that allow to a tumor cell answering to microenvironmental variations, and in this sense they are important contributors to tumor cell malignancy. Matricellular receptors represent thus valuable targets for the development of original anti-cancer strategies. Original reports, bibliographic reviews or hypotheses are welcome to improve the basic knowledge of matricellular receptor properties, their spatio-temporal regulation, the dynamic formation of complex receptors and the impact of such interactions on the invasive properties of tumor cells. Biological, biophysical and pharmacological, as well as in silico contributions will be appreciated.

Author: Fabio Doctorovich
Publisher: Elsevier
ISBN: 0128011645
Category : Science
Languages : en
Pages : 425

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Book Description
The Chemistry and Biology of Nitroxyl (HNO) provides first-of-its-kind coverage of the intriguing biologically active molecule called nitroxyl, or azanone per IUPAC nomenclature, which has been traditionally elusive due to its intrinsically high reactivity. This useful resource provides the scientific basis to understand the chemistry, biology, and technical aspects needed to deal with HNO. Building on two decades of nitric oxide and nitroxyl research, the editors and authors have created an indispensable guide for investigators across a wide variety of areas of chemistry (inorganic, organic, organometallic, biochemistry, physical, and analytical); biology (molecular, cellular, physiological, and enzymology); pharmacy; and medicine. This book begins by exploring the unique molecule's structure and reactivity, including important reactions with small molecules, thiols, porphyrins, and key proteins, before discussing chemical and biological sources of nitroxyl. Advanced chapters discuss methods for both trapping and detecting nitroxyl by spectroscopy, electrochemistry, and fluorescent inorganic cellular probing. Expanding on the compound's foundational chemistry, this book then explores its molecular physiology to offer insight into its biological implications, pharmacological effects, and practical issues. - Presents the first book on HNO (nitroxyl or azanone), an increasingly important molecule in biochemistry and pharmaceutical research - Provides a valuable coverage of HNO's chemical structure and significant reactions, including practical guidance on working with this highly reactive molecule - Contains high quality content from recognized experts in both industry and academia

Author: Mark S. Meskin
Publisher: CRC Press
ISBN: 1420031694
Category : Medical
Languages : en
Pages : 221

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Book Description
Are soy isoflavones neuroprotective? Just how different is one species of Echinacea from another? Which phytochemicals will be effective as therapeutic agents in vivo? Supported by solid scientific research, Phytochemicals in Nutrition and Health helps provide answers to these and other probing questions concerning the mechanisms of action associat