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Author: Jingcheng Huang Publisher: ISBN: 9781392365762 Category : Electronic dissertations Languages : en Pages : 164
Book Description
Electron transfer reactions are vital for life: they are the essential steps in all the major biological energy conservation pathways and the rate of electron transfer sometimes determines the fate of energy flow. While the rates of electron transfer over 1-2 nanometers in proteins can largely be described by well-known theories, it is not well understood how these processes scale to microscopic distances, for example, micrometer length microbial nanowires. Electron transfer reactions are known to be highly sensitive to the chemical properties of the electron carriers and distances between carriers, yet, this information is not available for naturally occurring microbial nanowires. On the other hand, microbial nanowires have inspired the development of novel biological conductive materials and bioelectronics, although these biomimicking materials would significantly benefit from a higher degree of structural definition, which would greatly improve rational redesign.This dissertation work presents two distinct approaches for arranging electron carriers (heme) into structurally defined arrays that can facilitate electron transfer:1) A crystalline lattice of small tetraheme cytochromes that form a well-defined, three-dimensional network of closely spaced redox centers was used to demonstrate the multi-step electron hopping over a micrometer scale.2) A heme attachment strategy was developed that allows one to introduce redox active cofactor hemes into non-heme-binding proteins, while maintaining the proteins' original function. Adding hemes to a nanotube-forming self-assembling protein was used to demonstrate the potential of this strategy to form a structurally defined heme array.The first crystal approach provides detailed information about structure and electronic states which can be used as a platform for testing theories, while the second heme-attaching approach is an engineering platform that allows researchers to introduce redox properties into other well-studied proteins with minimal effort. These two approaches, from two perspectives, lay the foundation of building structurally defined architectures for the understanding of microbial nanowires and the application of biological long-range electron transfer materials.
Author: Jingcheng Huang Publisher: ISBN: 9781392365762 Category : Electronic dissertations Languages : en Pages : 164
Book Description
Electron transfer reactions are vital for life: they are the essential steps in all the major biological energy conservation pathways and the rate of electron transfer sometimes determines the fate of energy flow. While the rates of electron transfer over 1-2 nanometers in proteins can largely be described by well-known theories, it is not well understood how these processes scale to microscopic distances, for example, micrometer length microbial nanowires. Electron transfer reactions are known to be highly sensitive to the chemical properties of the electron carriers and distances between carriers, yet, this information is not available for naturally occurring microbial nanowires. On the other hand, microbial nanowires have inspired the development of novel biological conductive materials and bioelectronics, although these biomimicking materials would significantly benefit from a higher degree of structural definition, which would greatly improve rational redesign.This dissertation work presents two distinct approaches for arranging electron carriers (heme) into structurally defined arrays that can facilitate electron transfer:1) A crystalline lattice of small tetraheme cytochromes that form a well-defined, three-dimensional network of closely spaced redox centers was used to demonstrate the multi-step electron hopping over a micrometer scale.2) A heme attachment strategy was developed that allows one to introduce redox active cofactor hemes into non-heme-binding proteins, while maintaining the proteins' original function. Adding hemes to a nanotube-forming self-assembling protein was used to demonstrate the potential of this strategy to form a structurally defined heme array.The first crystal approach provides detailed information about structure and electronic states which can be used as a platform for testing theories, while the second heme-attaching approach is an engineering platform that allows researchers to introduce redox properties into other well-studied proteins with minimal effort. These two approaches, from two perspectives, lay the foundation of building structurally defined architectures for the understanding of microbial nanowires and the application of biological long-range electron transfer materials.
Author: Claudio Nicolini Publisher: World Scientific ISBN: 9814725862 Category : Science Languages : en Pages : 336
Book Description
Molecular bioelectronics is a field in strong evolution at the frontier of life and materials sciences. The term is utilized in a broad context to emphasize a unique blend of electronics and biotechnology which is seen as the best way to achieve many objectives of industrial and scientific relevance, including biomolecular engineering, bioelectronic devices, materials and sensors capable of optimal hardware efficiency and intelligence and molecular miniaturization.
Author: G.W. Canters Publisher: Springer Science & Business Media ISBN: 9401151334 Category : Science Languages : en Pages : 290
Book Description
From May 3-7,1997, the NATO Advanced Research Workshop on 'Biological Electron Transfer Chains' was organized in Tomar, Portugal. In the application for support the choice of the topic was justified as follows: "[Until recently efforts] have concentrated on the study of the structure and function of individual redox enzymes and proteins. Enough information is now available to make a start with the study of biological electron transfer (E1) at the next higher level of organization, that of the complete ET chain." The interest in the workshop was high: the majority of participants had registered before the workshop was formally announced, which illustrates the popularity of the topic within the biochemical and biophysical communities. The present volume contains a number of reports based on the lectures presented by the key speakers during the meeting. The workshop dealt with the following three themes: a) Electron transfer, which is the subject of Chapter 1. The analysis of ET at the molecular level is still fundamental for an understanding of how ET chains operate in vivo. After 40 years of research the contours of the subject are becoming clear now. b) Bacterial redox chains. This is the subject of Chapter 2. Its contents show how complicated these chains can be, often involving a number of gene clusters. Our understanding of the regulatory aspects and control mechanisms of these chains is only in its beginning.
Author: Helmut Sigel Publisher: CRC Press ISBN: 9780824784942 Category : Science Languages : en Pages : 584
Book Description
Part of a series devoted to understanding the relationship between the chemistry of metals and life processes, the present volume offers contributions by 25 scientists covering mechanistic considerations, electron tunneling pathways, photoinduced and stereoselective effects in electron transfer reac
Author: Ignacio Rojas Publisher: Springer Nature ISBN: 3031349601 Category : Science Languages : en Pages : 520
Book Description
This volume constitutes the proceedings of the 10th International Work-Conference on IWBBIO 2023, held in Meloneras, Gran Canaria, Spain, during July 12-14, 2022. The total of 79 papers presented in the proceedings, was carefully reviewed and selected from 209 submissions. The papers cove the latest ideas and realizations in the foundations, theory, models, and applications for interdisciplinary and multidisciplinary research encompassing disciplines of computer science, mathematics, statistics, biology, bioinformatics, and biomedicine.
Author: Miyuki Thirumurthy Publisher: ISBN: Category : Bioelectrochemistry Languages : en Pages : 139
Book Description
Exoelectrogenic microorganisms can grow by transferring electrons from their internal metabolism to extracellular substrates in a process known as extracellular electron transfer (EET). This dissertation explores the mechanisms of EET by both chemotrophic and phototrophic organisms and constructs a novel supramolecular structure that can be used as a model for microbial, long-range electron transfer. Geobacter sulfurreducens has been hypothesized to secrete and use riboflavin as a soluble, extracellular redox shuttle in conjunction with multi-heme, outer membrane, c-type cytochromes, but the required proteins and their properties have not been defined. To address the mechanism of extracellular electron transfer by G. sulfurreducens, the first part of this work explores the interaction between an outer membrane, octaheme, c-type cytochrome OmcZs from G. sulfurreducens and riboflavin. Interrogation via multiple physical techniques shows that OmcZs transfers electrons to riboflavin. By analogy to other characterized systems, riboflavin then likely interacts with extracellular acceptors directly. The second part of this work addresses the mechanisms of EET by the model cyanobacterium Synechocystis sp. PCC 6803. It has been hypothesized that Synechocystis employs conductive pili for production of extracellular current. However, the results herein show that a strain that does not have pili produces extracellular photocurrent in a direct electrochemical cell at a level similar to that by wild type cells. Furthermore, conductive atomic force microscopy (AFM) imaging is used to show that pili produced by the wild type organism are not conductive. Thus, an alternative EET mechanism must be operable. In the third part of this work, a supramolecular structure comprised of peptide and cytochromes designed to serve as a model for long-range electron transfer through cytochrome rich environments is described. The c-type cytochromes in this synthetic nanowire retain their redox activity after assembly and have suitable characteristics for long-range electron transfer. Taken together, the results of this dissertation not only inform on natural microbial mechanisms for EET but also provide a starting point to develop novel, synthetic systems.
Author: Astrid Sigel Publisher: Routledge ISBN: 1351432133 Category : Science Languages : en Pages : 852
Book Description
Continues the tradition of excellence established in previous volumes in this acclaimed series. Volume 36 focuses on the vibrant research area concerning the interrelation between free radicals and metal ions and their resulting effects on life processes; it offers an authoritative and timely account of this fascinating area of research in 21 chapters.
Author: Joshua Jortner Publisher: Wiley-Interscience ISBN: Category : Science Languages : en Pages : 812
Book Description
an integrated approach to electron transfer phenomena This two-part stand-alone volume in the prestigious Advances in Chemical Physics series provides the most comprehensive overview of electron transfer science today. It draws on cutting-edge research from diverse areas of chemistry, physics, and biology-covering the most recent developments in the field, and pointing to important future trends. This initial volume includes: * A historical perspective spanning five decades * A review of concepts, problems, and ideas in current research * Electron transfer in isolated molecules and in clusters * General theory, including useful algorithms * Spectra and electron transfer kinetics in bridged compounds The second volume covers solvent control, ultrafast electron transfer and coherence effects, molecular electronics, electron transfer and chemistry, and biomolecules. Electron transfer science has seen tremendous progress in recent years. Technological innovations, most notably the advent of femtosecond lasers, now permit the real-time investigation of intramolecular and intermolecular electron transfer processes on a time scale of nuclear motion. New scientific information abounds, illuminating the processes of energy acquisition, storage, and disposal in large molecules, clusters, condensed phase, and biophysical systems. Electron Transfer: From Isolated Molecules to Biomolecules is the first book devoted to the exciting work being done in nonradiative electron transfer dynamics today. This two-part edited volume emphasizes the interdisciplinary nature of the field, bringing together the contributions of pioneers in chemistry, physics, and biology. Both theoretical and experimental topics are featured. The authors describe modern approaches to the exploration of different systems, including supersonic beam techniques, femtosecond laser spectroscopy, chemical syntheses, and methods in genetic and chemical engineering. They examine applications in such areas as supersonic jets, solvents, electrodes, semi- conductors, respiratory and enzymatic protein systems, photosynthesis, and more. They also relate electron transfer and radiationless transitions theory to pertinent physical phenomena, and provide a conceptual framework for the different processes. Complete with over two hundred illustrations, Part One reviews developments in the field since its inception fifty years ago, and discusses electron transfer phenomena in both isolated molecules and in clusters. It outlines the general theory, exploring areas of the control of kinetics, structure-function relationships, fluctuations, coherence, and coupling to solvents with complex spectral density in different types of electron transfer processes. Timely, comprehensive, and authoritative, Electron Transfer: From Isolated Molecules to Biomolecules is an essential resource for physical chemists, molecular physicists, and researchers working in nonradiative dynamics today.
Author: Jingcheng Huang
Author: Jingcheng Huang
Author: 
Author: 
Author: Claudio Nicolini
Author: G.W. Canters
Author: Helmut Sigel
Author: Ignacio Rojas
Author: Miyuki Thirumurthy
Author: Astrid Sigel
Author: Joshua Jortner