Author: Harmin Herrera Publisher: ISBN: Category : Biochemical markers Languages : en Pages : 241
Book Description
Changes in serum glycosylation have long been associated with hepatocellular carcinoma (HCC), and these modifications are often detected with sugar-binding proteins called lectins. HCC is associated with increased serum fucosylation, and this sugar is often detected with the Aleuria aurantia lectin (AAL). However, this lectin binds fucose in several forms, one of which is associated with cancer ([alpha]1-6 linked fucosylation) and some of which are not. Thus, its use in clinical and tissue analysis is limited. We have created a recombinant AAL, which contains a single amino acid mutation: N224Q (rAALN224Q), within the binding site 5, and this lectin has increased sensitivity to the cancer-specific fucosylated glycan. Our analysis of rAALN224Q has shown that the mutation within one specific fucose binding pocket had a major impact upon the mutated site as well as on sites that were not mutated and led to a lectin with altered binding specificities. This lectin is currently part of a diagnostic test under evaluation for the early detection of HCC. We have further explored the changes in N-linked glycosylation associated directly with HCC tissue through a method of N-linked glycan matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS). MALDI IMS of N-linked glycan revealed inter- and intra-tumor heterogeneity shown by expression and spatial localization of glycans in the tumor. Tumors were associated with alterations in mannosylation, fucosylation, branching, and complex glycan formation. Importantly, all tumors were associated with some change in glycosylation, but there was significant glycan heterogeneity.
Author: Ajit Varki Publisher: CSHL Press ISBN: 9780879696818 Category : Medical Languages : en Pages : 694
Book Description
Sugar chains (glycans) are often attached to proteins and lipids and have multiple roles in the organization and function of all organisms. "Essentials of Glycobiology" describes their biogenesis and function and offers a useful gateway to the understanding of glycans.
Author: A. Pusztai Publisher: Cambridge University Press ISBN: 0521328241 Category : Medical Languages : en Pages : 276
Book Description
This volume surveys the chemistry, biochemistry, biosynthesis, metabolism and pharmacological properties of lectins. Lectins, which are most commonly found in plants, are widespread natural products with striking biological activities. Their specific ability to recognise and bind to simple or complex saccharides facilitates their role as effective information protein molecules. As agents of cell-to-cell recognition, lectins promote symbiosis between plants and specific nitrogen-fixing soil bacteria. As natural defensive molecules, they can protect plants against predators such as bacteria, fungi and insects. As part of our diet, lectins are powerful exogenous growth factors in the small intestine and influence our health, the digestive function and the bacterial ecology of the alimentary tract. Lectins are also important research tools in preparative biochemistry and cell science.
Author: Publisher: Academic Press ISBN: 0128016140 Category : Science Languages : en Pages : 418
Book Description
Advances in Cancer Research provides invaluable information on the exciting and fast-moving field of cancer research. Here, once again, outstanding and original reviews are presented on a variety of topics. Provides information on cancer research Outstanding and original reviews Suitable for researchers and students
Author: Publisher: Academic Press ISBN: 012817739X Category : Science Languages : en Pages : 418
Book Description
Glycans and Glycosaminoglycans as Clinical Biomarkers and Therapeutics - Part A, Volume 162 in the Progress in Molecular Biology and Translational Science series provides informative monographs on a variety of research topics related to Glycans and glycosaminoglycans as clinical biomarkers and therapeutics. Topics in this update include Glycan-based Biomarkers for Diagnosis of Cancers and Other Diseases: Past, Present and Future, Desialylation in Diseases and its Application in Diagnostic and Therapeutic Development, Proteoglycans as Miscommunication Biomarkers for Cancer Diagnosis, Fucosylation in Cancer Biology and Its Clinical Applications, Retrospective Analysis of Glycan-Related Biomarkers Based on Clinical Laboratory Data in Two Medical Centers, and many related topics. Includes comprehensive coverage of molecular biology Presents ample use of tables, diagrams, schemata and color figures to enhance the reader's ability to rapidly grasp the information provided Contains contributions from renowned experts in the field
Author: Evandro Fei Fang Publisher: Springer ISBN: 9789401785297 Category : Medical Languages : en Pages : 0
Book Description
The modern unhealthy diet and lifestyle in conjunction with pathogens, environmental carcinogens and multiple other risk factors increase humans’ susceptibility to different diseases exemplified by elevated levels of cancers, cardiovascular and communicable diseases. Screening of potential drugs from medicinal plants and animals provides a promising strategy for the alleviation of the impact of these diseases. Components with potential medicinal applications include RIPs, RNases, lectins, protease inhibitors and numerous small compounds. These compounds have shown both preventive and therapeutic effects for humans. This book is a compilation of articles written by internationally renowned experts exploring the different uses of medicinal compounds in human therapeutics. Here we provide a comprehensive outlook on both qualitative and quantitative studies focusing on medicinal plants and animals, and establishing a link between laboratory research discovery and clinical applications.
Author: Binghe Wang Publisher: John Wiley & Sons ISBN: 0470592079 Category : Medical Languages : en Pages : 448
Book Description
This book contains contributions from interdisciplinary scientists to collectively address the issue of targeting carbohydrate recognition for the development of novel therapeutic and diagnostic agents. The book covers (1) biological problems involving carbohydrate recognition, (2) structural factors mediating carbohydrate recognition, (3) design and synthesis of lectin mimics that recognize carbohydrate ligands with high specificity and affinity, and (4) modulation of biological and pathological processes through carbohydrate recognition.
Author: Minoru Fukuda Publisher: CRC Press ISBN: 9780849364358 Category : Science Languages : en Pages : 342
Book Description
Cell Surface Carbohydrates and Cell Development summarizes knowledge on the structure and function of cell surface carbohydrates in development and differentiation. The chapters include reviews on the expression of cell type-specific carbohydrates and their roles in cell-cell interaction. In particular, the role of cell surface carbohydrates in immune cell response, malignant transformation, fertilization, and neural cell development are addressed. This includes the exciting discovery about the role of adhesive molecules in leukocyte-endothellium interaction. Cell Surface Carbohydrates and Cell Development also summarizes the latest knowledge on structure and biosynthesis of carbohydrates, the role of specific carbohydrate modification, and animal lectins. The book will be useful to researchers and students interested in the biology of glycoproteins and biotechnology.
Author: Timothy D. Veenstra Publisher: John Wiley & Sons ISBN: 0470007737 Category : Science Languages : en Pages : 361
Book Description
Written by recognized experts in the study of proteins, Proteomics for Biological Discovery begins by discussing the emergence of proteomics from genome sequencing projects and a summary of potential answers to be gained from proteome-level research. The tools of proteomics, from conventional to novel techniques, are then dealt with in terms of underlying concepts, limitations and future directions. An invaluable source of information, this title also provides a thorough overview of the current developments in post-translational modification studies, structural proteomics, biochemical proteomics, microfabrication, applied proteomics, and bioinformatics relevant to proteomics. Presents a comprehensive and coherent review of the major issues faced in terms of technology development, bioinformatics, strategic approaches, and applications Chapters offer a rigorous overview with summary of limitations, emerging approaches, questions, and realistic future industry and basic science applications Discusses higher level integrative aspects, including technical challenges and applications for drug discovery Accessible to the novice while providing experienced investigators essential information Proteomics for Biological Discovery is an essential resource for students, postdoctoral fellows, and researchers across all fields of biomedical research, including biochemistry, protein chemistry, molecular genetics, cell/developmental biology, and bioinformatics.
Author: Harmin Herrera
Author: Ajit Varki
Author: A. Pusztai
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Author: Evandro Fei Fang
Author: Binghe Wang
Author: Minoru Fukuda
Author: Azita Alavi
Author: Timothy D. Veenstra