Development of a Fate Map for the Vascular System in the 16-cell Embryo of Xenopus Laevis PDF Download

Author: Kenna Mills
Publisher:
ISBN:
Category : Embryology
Languages : en
Pages : 104

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Author: Hazel L. Sive
Publisher: CSHL Press
ISBN: 9780879695040
Category : Medical
Languages : en
Pages : 370

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Book Description
Amphibian embryos are supremely valuable in studies of early vertebrate development because they are large, handle easily, and can be obtained at many interesting stages. And of all the amphibians available for study, the most valuable is Xenopus laevis,which is easy to keep and ovulates at any time of year in response to simple hormone injections. Xenopusembryos have been studied for years but this is a particularly exciting time for the field. Techniques have become available very recently that permit a previously impossible degree of manipulation of gene expression in intact embryos, as well as the ability to visualize the results of such manipulation. As a result, a sophisticated new understanding of Xenopusdevelopment has emerged, which ensures the species’ continued prominent position among the organisms favored for biological investigation. This manual contains a comprehensive collection of protocols for the study of early development in Xenopusembryos. It is written by several of the field’s most prominent investigators in the light of the experience they gained as instructors in an intensive laboratory course taught at Cold Spring Harbor Laboratory since 1991. As a result it contains pointers, hints, and other technical knowledge not readily available elsewhere. This volume is essential reading for all investigators interested in the developmental and cell biology of Xenopusand vertebrates generally. Many of the techniques described here are illustrated in an accompanying set of videotapeswhich are cross-referenced to the appropriate section of the manual.

Author: Candace Tamara Myers
Publisher:
ISBN:
Category :
Languages : en
Pages : 394

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Each step of vascular development needs to be carefully regulated; endothelial precursors must be specified, these cells then proliferate and coalesce to form vascular cords, and finally they lumenate, undergo angiogenic branching and remodeling, and recruit smooth muscle cells to establish a mature vessel. An aberration at any of these steps during embryonic development is incompatible with life, and vascular pathologies in the adult are associated with numerous diseases including stroke, arteriosclerosis, diabetic retinopathies and cancer progression. My work has aimed to understand how endothelial precursors are specified, and more precisely the cell-signaling pathways and transcriptional networks that guide their fate. This work leads us to conclude the following: (1) blood island precursor cells in the Xenopus embryo can give rise to either blood or endothelial cells, and it is BMP-mediated activation of the erythroid transcriptional program that regulates cell fate, (2) endothelial specification requires the Ets transcription factor Etv2. Persistence of Etv2 expression in blood/endothelial cell precursors allows these cells to develop into endothelium, and overexpression of Etv2 in any of the three germ layers causes activation of every endothelial marker examined. Along the way we have characterized a number of small-molecule inhibitors that should be useful to the Xenopus community and applicable to other model systems.

Author: Malgorzata Kloc
Publisher: John Wiley & Sons
ISBN: 111849282X
Category : Science
Languages : en
Pages : 459

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Book Description
Frogs from the genus Xenopus have long been used as model organisms in basic and biomedical research. These frogs have helped unlock key fundamental developmental and cellular processes that have led to important scientific breakthroughs and have had practical application in embryology, cancer research and regenerative medicine. Xenopus Development is a vital resource on the biology and development of these key model organisms, and will be a great tool to researchers using these frogs in various disciplines of biological science. Xenopus Development is divided into four sections, the first three highlight key processes in Xenopus development from embryo to metamophosis. These sections focus on the cellular processes, organogenesis and embryo development. The final section highlights novel techniques and approaches being used in Xenopus research. Providing thorough and detailed coverage, Xenopus Development, will be a timely and welcome volume for those working in cell and molecular biology, genetics, developmental biology and biomedical research. Provides broad overview of the developmental biology of both Xenopus laevis and Xenopus tropicalis Explores cellular to systems development in key biomedical model organisms Timely synthesis of the field of Xenopus biology Highlights key biomedical and basic biological findings unlocked by Xenopus

Author: Peter Hausen
Publisher: Springer
ISBN:
Category : Medical
Languages : en
Pages : 158

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Author: Paul A. Wilson
Publisher:
ISBN:
Category :
Languages : en
Pages : 394

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Author: Peter D. Vize
Publisher: Elsevier
ISBN: 0080521541
Category : Science
Languages : en
Pages : 534

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Book Description
Organogenesis of the kidney has been intensely studied for over a century. In recent years advances in molecular techniques have not only made great inroads into exploring the genetic regulation of this complex process but also began to unravel the molecular basis of many forms of congenital kidney disease. This book is a comprehensive study on these findings and the only book available with such in depth coverage of the kidney. Hundreds of color figures depicting key events in all aspects of kidney development Full coverage of the genetic and cellular basis of kidney development Analysis of the genetic basis of the major congenital kidney diseases

Author: P. Michael Conn
Publisher: Springer Science & Business Media
ISBN: 1597452858
Category : Medical
Languages : en
Pages : 756

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Book Description
The collection of systems represented in this volume is a unique effort to reflect the diversity and utility of models used in biomedicine. That utility is based on the consideration that observations made in particular organisms will provide insight into the workings of other, more complex systems. This volume is therefore a comprehensive and extensive collection of these important medical parallels.

Author: Pieter D. Nieuwkoop
Publisher: Garland Science
ISBN: 9780815318965
Category : Medical
Languages : en
Pages : 252

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Book Description
A Systematical and Chronological Survey of the Development from the Fertilized Egg till the End of Metomorphosis

Author: Jonathan M. W. Slack
Publisher: John Wiley & Sons
ISBN: 1118387600
Category : Science
Languages : en
Pages : 496

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Book Description
Essential Developmental Biology is a comprehensive, richly illustrated introduction to all aspects of developmental biology. Written in a clear and accessible style, the third edition of this popular textbook has been expanded and updated In addition, an accompanying website provides instructional materials for both student and lecturer use, including animated developmental processes, a photo gallery of selected model organisms, and all artwork in downloadable format. With an emphasis throughout on the evidence underpinning the main conclusions, this book is an essential text for both introductory and more advanced courses in developmental biology. Shortlisted for the Society of Biology Book Awards 2013 in the Undergraduate Textbook category. Reviews of the Second Edition: "The second edition is a must have for anyone interested in development biology. New findings in hot fields such as stem cells, regeneration, and aging should make it attractive to a wide readership. Overall, the book is concise, well structured, and illustrated. I can highly recommend it." —Peter Gruss, Max Planck Society "I have always found Jonathan Slack's writing thoughtful, provocative, and engaging, and simply fun to read. This effort is no exception. Every student of developmental biology should experience his holistic yet analytical view of the subject." —Margaret Saha, College of William & Mary