Development and Application of Novel Matrices Under Matrix-assisted Laser Desorption/ionization Time-of-flight (MALDI-TOF) Mass Spectrometry Conditions PDF Download

Author: Bharat R. Mankani
Publisher:
ISBN:
Category : Matrix-assisted laser desorption-ionization
Languages : en
Pages : 176

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Author: Rainer Cramer
Publisher: Springer
ISBN: 3319048198
Category : Science
Languages : en
Pages : 287

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Book Description
This book covers the state-of-the-art of modern MALDI (matrix-assisted laser desorption/ionization) and its applications. New applications and improvements in the MALDI field such as biotyping, clinical diagnosis, forensic imaging, and ESI-like ion production are covered in detail. Additional topics include MS imaging, biotyping/speciation and large-scale, high-speed MS sample profiling, new methods based on MALDI or MALDI-like sample preparations, and the advantages of ESI to MALDI MS analysis. This is an ideal book for graduate students and researchers in the field of bioanalytical sciences. This book also: • Showcases new techniques and applications in MALDI MS • Demonstrates how MALDI is preferable to ESI (electrospray ionization) • Illustrates the pros and cons associated with biomarker discovery studies in clinical proteomics and the various application areas, such as cancer proteomics

Author: Kandalama KD Priyasantha
Publisher:
ISBN:
Category : Biochemical markers
Languages : en
Pages : 246

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Book Description
Matrix Assisted Laser Desorption / Ionization (MALDI) Mass Spectrometry (MS) has emerged as an important tool in the field of proteomics mainly because it is simple, quick and efficient. The identification and quantitation of biomarkers, protein targets for drugs, and metabolites are some of the important fields in proteomics research. Although MALDI MS is an important tool in proteomics research there are drawbacks of the technique that need further development in order for the approach to be used in clinical laboratories. One major limitation of MALDI MS is the generally poor reproducibility of ion signal intensities, which negatively impacts the quantitation of peptides and protein by MALDI MS. A considerable amount of research has been performed in an effort to improve the ion signal reproducibility in MALDI MS. However, many of the approaches developed have introduced specific drawbacks with respect to the traditional dried-droplet sample preparation technique, negating many of the advantages of the MALDI MS approach. This project has focused on the development of a novel approach to quantify peptides by MALDI MS while preserving traditional known advantages of the technique. The studies performed show that an approach in which the ion signal base widths are manipulated to match that of a reference ion signal, through adjustments in desorption laser intensity, leads to much higher reproducibility in the integrated ion signal intensities. A standard curve acquired using the constant ion signal base width approach showed lower average RSDs ( 10.00% vs. 39.00%) and improved R 2 values (> 0.9600 vs.

Author: Franz Hillenkamp
Publisher: Wiley-Blackwell
ISBN: 9783527314409
Category : Science
Languages : en
Pages : 0

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Book Description
"The introduction of the matrix-assisted laser desorption ionization technique (MALDI) changed mass spectrometry (MS) into a powerful tool for biomedicial analysis that is now widely employed in academic as well as industrial laboratories. - This book gives an in-depth description of the many different applications of MALDI MS, along with a detailed discussion of the technology itself. - It will be a much-needed practical and educational asset for individuals, academic institutions and companies in the field of bioanalytics."--BOOK JACKET.

Author: Elizabeth Bauerle
Publisher:
ISBN:
Category :
Languages : en
Pages : 67

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Book Description
MALDI-TOF-MS has the ability to streamline identification of microorganisms in the microbiology lab. In this project, we focused on two main aims. First, the implementation of this technology for direct blood culture identification. Within this aim we determined the limit of detection for the protein purification method we planned on using in our laboratory, determined handling conditions for the samples, and evaluated the MALDI-TOF-MS for concordance with our current workflow and reports. Second, customization of the commercial library for Cryptococcus gattii was performed. Within this aim, we improved representation of the species in the MALDI-TOF-MS library, and also explored ways to determine the subspecies based on gathered spectra.

Author: Yongdong Zhu
Publisher:
ISBN:
Category :
Languages : en
Pages : 364

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Book Description

Author: Franz Hillenkamp
Publisher: John Wiley & Sons
ISBN: 3527333312
Category : Science
Languages : en
Pages : 0

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Book Description
This authoritative book on MALDI MS, now finally available in its second edition and edited by one of its inventors, gives an in-depth description of the many different applications, along with a detailed discussion of the technology itself. Thoroughly updated and expanded, with contributions from key players in the field, this unique book provides a comprehensive overview of MALDI MS along with its possibilities and limitations. The initial chapters deal with the technology and the instrumental setup, followed by chapters on the use of MALDI MS in protein research (including proteomics), genomics, glycomics and lipidomics. The option of MALDI-MS for the analysis of polymers and small molecules are also covered in separate chapters, while new to this edition is a section devoted to the interplay of MALDI MS and bioinformatics. A much-needed practical and educational asset for individuals, academic institutions and companies in the field of bioanalytics.

Author: W. W. Christie
Publisher: Elsevier
ISBN: 0857097865
Category : Technology & Engineering
Languages : en
Pages : 447

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Book Description
This well-known and highly successful book was first published in 1973 and has been completely re-written in subsequent editions (published in 1982 and 2003). This new Fourth Edition has become necessary because of the pace of developments in mass spectrometry of intact lipids, which has given recognition of lipid analysis and ‘lipidomics’ as a distinct science. To bring the book up to date with these developments, author William W. Christie is joined by co-author Xianlin Han. Although devoting considerable space to mass spectrometry and lipidomics, Lipid analysis remains a practical guide, in one volume, to the complexities of the analysis of lipids. As in past editions, it is designed to act as a primary source, of value at the laboratory bench rather than residing on a library shelf. Lipid analysis deals with the isolation, separation, identification and structural analysis of glycerolipids, including triacylglycerols, phospholipids, sphingolipids, and the various hydrolysis products of these. The chapters follow a logical sequence from the extraction of lipids to the isolation and characterization of particular lipid classes and of molecular species of each, and to the mass spectrometric analysis of lipids and lipidomics. The new influence of mass spectrometry is due mainly to the development of electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). Most emphasis in this book is placed on ESI, which is enabling structural characterization of different lipid classes and the identification of novel lipids and their molecular species.

Author: Sajid Bashir
Publisher:
ISBN:
Category :
Languages : en
Pages :

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Author: Teresa Catherine Cain
Publisher:
ISBN:
Category :
Languages : en
Pages : 234

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Book Description