Determining Alternative and Sustainable Management Strategies to Manage the Northern Root-knot Nematode (Meloidogyne Hapla) in Ornamental Plant Production Fields PDF Download
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Author: Amanda D. Howland Publisher: ISBN: Category : Electronic dissertations Languages : en Pages : 0
Book Description
The United States floriculture industry was valued at $6.43 billion in 2021, with Michigan being the third largest producer, producing 10% of all ornamental plants in the United States. A major constraint to the production of bare-rooted ornamental plants grown in the field are plant-parasitic nematodes. In Michigan, plant-parasitic nematodes cause millions of dollars in economic loss each year in the state's $104.7 billion agriculture industry. In the northern United States and Canada, the northern root-knot nematode, Meloidogyne hapla, is the most economically important perennial ornamental pathogen. While this is a known major pathogen of daylily production, one of top commodities in ornamental plant production in Michigan, very little is known about its impact in daylily production fields or how to effectively manage this pest. There are only two main management strategies for M. hapla in ornamental plant fields: hot water dips and preplant fumigation, both of which do not control M. hapla the entire production cycle and are therefore only semi-effective. Therefore, research was conducted to determine alternative management strategies to manage M. hapla in daylily production fields, with the goal to prevent yield loss and exportation rejection, and reduce the economic burden of this pest. Three multi-year field trials at a commercial nursery in Zeeland, MI, and several greenhouse experiments at Michigan State University's Plant Greenhouses, East Lansing, MI, were conducted to test several different management options and combination of management options to find the best new management strategies to control M. hapla in ornamental plant fields. The results of these studies demonstrate that there are more effective solutions for M. hapla management in ornamental plant field production compared to current practices and highlight three new management options: Indemnify as a soil drench, Majestene 304, and TerraClean 5.0 have been shown to provide the best M. hapla management in daylily fields, with a reduction in M. hapla population levels by 39.5%, 34.7%, and 28.8%, respectively, compared to the control. Indemnify also reduced the number of galled roots by 80% compared to the control plants, which is considerable and can lead to less fields being quarantined and fewer shipment rejections, significantly increasing the profits of the ornamental plant industry. The Indemnify treatment was additionally shown to have a significant positive effect on plant growth, producing plants with some of the largest overall plant biomass, such as plant heights, shoot weights, crown widths, and, most importantly, yield. Plants where Indemnify was applied as a soil drench always had higher yields (on average 41.3% higher) compared to the control plants and higher yields (on average 40% higher) compared to Telone II fumigation. These experiments also show that the annual application of treatments throughout the production cycle is crucial and provides significantly better M. hapla management compared to current practices, which only focuses on managing nematodes at the beginning of the production cycle. Most importantly, these trials show that there was no impact on plant growth, health, and yield from annual treatment applications. Even though M. hapla is well established in these monoculture, long-term ornamental plant fields, a trial determining possible soil suppression showed that natural suppression may not be occurring in ornamental plant fields in Michigan, but more experiments are needed. Two greenhouse trials tested the damage potential and host status of Hemerocallis spp. to M. hapla and Paratylenchus spp., and determined the threshold level of M. hapla. These greenhouse experiments show that daylily is an excellent host to M. hapla, with a threshold level as low as 13 M. hapla/100 cm3 soil. The data also suggests that even though M. hapla affects plant growth, daylily plants may actually be tolerant to M. hapla; over the length of the daylily growth cycle, the plants became more tolerant of its feeding and grew to similar sizes of the nematode-free plants. Lastly, daylily was shown to not be a host to Paratylenchus spp., and therefore, these nematodes do not need to be included in management decisions. Through the application of the new alternative and more sustainable management strategies described in this dissertation, M. hapla can be effectively and efficiently managed in ornamental plant fields leading to a significant advancement in the floriculture industry in Michigan, the northern United States, and Canada.
Author: Amanda D. Howland Publisher: ISBN: Category : Electronic dissertations Languages : en Pages : 0
Book Description
The United States floriculture industry was valued at $6.43 billion in 2021, with Michigan being the third largest producer, producing 10% of all ornamental plants in the United States. A major constraint to the production of bare-rooted ornamental plants grown in the field are plant-parasitic nematodes. In Michigan, plant-parasitic nematodes cause millions of dollars in economic loss each year in the state's $104.7 billion agriculture industry. In the northern United States and Canada, the northern root-knot nematode, Meloidogyne hapla, is the most economically important perennial ornamental pathogen. While this is a known major pathogen of daylily production, one of top commodities in ornamental plant production in Michigan, very little is known about its impact in daylily production fields or how to effectively manage this pest. There are only two main management strategies for M. hapla in ornamental plant fields: hot water dips and preplant fumigation, both of which do not control M. hapla the entire production cycle and are therefore only semi-effective. Therefore, research was conducted to determine alternative management strategies to manage M. hapla in daylily production fields, with the goal to prevent yield loss and exportation rejection, and reduce the economic burden of this pest. Three multi-year field trials at a commercial nursery in Zeeland, MI, and several greenhouse experiments at Michigan State University's Plant Greenhouses, East Lansing, MI, were conducted to test several different management options and combination of management options to find the best new management strategies to control M. hapla in ornamental plant fields. The results of these studies demonstrate that there are more effective solutions for M. hapla management in ornamental plant field production compared to current practices and highlight three new management options: Indemnify as a soil drench, Majestene 304, and TerraClean 5.0 have been shown to provide the best M. hapla management in daylily fields, with a reduction in M. hapla population levels by 39.5%, 34.7%, and 28.8%, respectively, compared to the control. Indemnify also reduced the number of galled roots by 80% compared to the control plants, which is considerable and can lead to less fields being quarantined and fewer shipment rejections, significantly increasing the profits of the ornamental plant industry. The Indemnify treatment was additionally shown to have a significant positive effect on plant growth, producing plants with some of the largest overall plant biomass, such as plant heights, shoot weights, crown widths, and, most importantly, yield. Plants where Indemnify was applied as a soil drench always had higher yields (on average 41.3% higher) compared to the control plants and higher yields (on average 40% higher) compared to Telone II fumigation. These experiments also show that the annual application of treatments throughout the production cycle is crucial and provides significantly better M. hapla management compared to current practices, which only focuses on managing nematodes at the beginning of the production cycle. Most importantly, these trials show that there was no impact on plant growth, health, and yield from annual treatment applications. Even though M. hapla is well established in these monoculture, long-term ornamental plant fields, a trial determining possible soil suppression showed that natural suppression may not be occurring in ornamental plant fields in Michigan, but more experiments are needed. Two greenhouse trials tested the damage potential and host status of Hemerocallis spp. to M. hapla and Paratylenchus spp., and determined the threshold level of M. hapla. These greenhouse experiments show that daylily is an excellent host to M. hapla, with a threshold level as low as 13 M. hapla/100 cm3 soil. The data also suggests that even though M. hapla affects plant growth, daylily plants may actually be tolerant to M. hapla; over the length of the daylily growth cycle, the plants became more tolerant of its feeding and grew to similar sizes of the nematode-free plants. Lastly, daylily was shown to not be a host to Paratylenchus spp., and therefore, these nematodes do not need to be included in management decisions. Through the application of the new alternative and more sustainable management strategies described in this dissertation, M. hapla can be effectively and efficiently managed in ornamental plant fields leading to a significant advancement in the floriculture industry in Michigan, the northern United States, and Canada.
Author: Isaac Lartey Publisher: ISBN: Category : Electronic dissertations Languages : en Pages : 0
Book Description
Managing Meloidogyne hapla remains challenging due to the ban of broad-spectrum nematicides, lack of resistant crops and its broad host range. It also has parasitic variability (PV) where populations (pop) are morphologically and genetically similar but vary in pathogenicity and reproductive potential. Although PV in M. hapla appears to have some relationship to soil types, what soil conditions favor its PV and/or its distribution are unknown. The goal of my research was to understand the soil conditions where M. hapla PVexist by quantifying the biophysicochemical (BPC) conditions from the ecosystem down to microbiome level. I designed observational and experimental approaches and tested four objectives. First, was to evaluate the association between soil conditions and M. hapla distribution at the ecosystem level. My hypothesis was that the presence of M. hapla will be associated with degraded soil conditions. I selected 15 (6 muck and 9 mineral soil)agricultural fields with adjacent natural vegetation in southwest, northwest and eastern regions of the lower peninsula of Michigan as study sites. I collected a total of 75 (5 per field)georeferenced soil samples from agricultural fields and equal number from adjacent natural vegetation soils, quantified the soil food web (SFW) conditions using the Ferris SFW model, and screened for M. hapla presence or absence. The fields were described either as disturbed, degraded (worst-case) or maturing (best-case). Meloidogyne hapla was present in 3 mineral (2, 8 and 13) and 6 muck (4, 5, 6, 10, 14 15) agricultural fields with degraded and/or disturbed soil conditions and absent from maturing soils, partially supporting the hypothesis.Degraded soils had low nitrogen content in both soil groups. The second objective was to isolate and culture the 9 M. hapla populations to test a hypothesis that PV is related to specific SFW conditions. I found three categories of reproductive potential: the highest (Pop 13), medium (Pop 8), both from degraded mineral soils, and lowest from disturbed mineral (Pop 2) and disturbed (Pops 4, 6 and 10) and degraded (Pops 5, 14 and 15) muck soils. Thus, the hypothesis was not supported. The third objective, was to determine relationships between microbial community structure and M. hapla distribution. My working hypotheses were that there is a relationship among microbiome, soil health and M. hapla occurrence. Microbial community structure in the fields was determined from sub-samples of the same samples where the nematodes were isolated. I used 16S (bacteria) and ITS (fungi) rDNA analysis and characterized the microbial composition, core- and indicator-microbes co-existing with M. hapla pop in the field soils and soil conditions relative to the Ferris SFW model description. The results showed that bacterial and fungal community abundance and composition varied by soil group, SFW conditions and/or M. hapla occurrence. I found that a core of 39 bacterial and 44 fungal sub-operational taxonomic units (OTUs) were found variably, 25 bacterial OTUs associated with presence or absence of M. hapla, and 1,065 OTUs were associated SFW conditions. All three hypotheses were supported. The final objective was to determine the relationship between PV and the microbes associated with M. hapla pop. I compared bacteria present in M. hapla pop isolated from the field and greenhouse cultures. The hypothesis was that either presence and/or absence of specific bacteria are associated with M. hapla population. Population 8 shared more bacteria with the lowest reproductive potential pop than Population 13. Presence of several bacteria was unique to Population 8 as was the absence of other bacteria to Pop 13 in either field or greenhouse nematodes. Therefore, the hypothesis was supported. My research findings provide a foundation for: a) testing the relationship between M. hapla PV and the BPC conditions and b) designing soil health-based management strategies.
Author: Kamal Kishore Chaudhary Publisher: Springer Nature ISBN: 3031099435 Category : Science Languages : en Pages : 439
Book Description
The present work covers many aspects of plant nematode management using organic strategies. These range from applications of latest understandings of fundamental concepts/mechanisms of nematode control, to modern tools and techniques used in efficacy evaluation. The Volume also includes some case studies/applied aspects of organic nematode pest management. Chemical and physical control measures used for nematode management have their own implications. Against this backdrop, organic management of plant nematodes appears as a more rational and sustainable approach. However, concise information on the current topic is scarce. This book is a sincere effort to bridge this void as we aim to provide the most updated, critical and in-depth knowledge synthesized by many experts working in the field of plant nematology, worldwide.
Author: Rosa H. Manzanilla-López Publisher: Springer ISBN: 3319592246 Category : Science Languages : en Pages : 418
Book Description
This volume reviews our current knowledge and novel research areas on Pochonia chlamydosporia, a cosmopolitan fungus occurring in soils as a saprophyte yet capable of colonizing the rhizosphere of crops as an endophyte and behaving as a parasite of eggs of plant-parasitic nematodes. The book is divided into six sections containing 18 chapters, starting with a historical background chapter, followed by 16 chapters, each contributed by experts, concerning those key aspects necessary to work with this biocontrol agent in a multidisciplinary treatise. Topics covered include systematics, biology, nematode-fungus interactions, nematode management strategies, secondary metabolites, and other methods including more novel research areas such as molecular, –omics, plant growth enhancement and endophytic abilities of P. chlamydosporia. The final chapter deals with the future perspectives of P. chlamydosporia research.
Author: Hassan Eshtiaghi Publisher: ISBN: Category : Root-knot Languages : en Pages : 152
Book Description
The northern root-knot nematode (Meloidogyne hapla Chitwood, 1949) is a widespread pest on many plants in temperate zones such as the Pacific Northwest (U.S.A.) Peppermint and spearmint are two major agricultural crops in Oregon and Washington with a combined annual acreage of 50, 000 to 55, 000 and an oil value of about 25 million dollars. Both plants are attacked by a number of natural enemies including some species of plant parasitic nematodes with M. hapla considered to be most destructive. A pathogenicity study which included fifteen different isolates of M. hapla, revealed that peppermint was a more susceptible host plant than spearmint. These nematode populations were collected from four major mint growing areas of the United States (Idaho, Indiana, Oregon and Washington) with most populations obtained from Oregon and Washington. Usually no symptoms appear on the above ground parts of root-knot nematode infected plants. Therefore, it is possible that farmers in these areas may overlook infected mint planting stock and introduce the pest into new lands. Spread of the nematode could cause serious problems in mint and many other agricultural crops. Inoculation of peppermint cuttings and rhizomes with different densities of nematode egg masses caused varying degrees of root gall formation in three and four month growing periods. Regression slopes indicated that the relationships between inoculum densities and root gall formation was significant at the one percent level. However, inoculated rhizomes and the longer growing period caused more infection in plants. A gradual decline in plant vigor and productivity could be attributed to root-knot nematode infection which resulted in a significant shoot length reduction and reduced plant weights. Dry matter contents and concentrations of N, P, Mn, Fe and Al decreased, but there was a slight increase in Ca. Spearmint rhizomes inoculated with fresh second stage larvae of M. hapla produced equally severe galling in four months of growth regardless of the various initial inoculum densities. However, no significant relationships between inoculum densities and reduction in plant weights or shoot lengths developed in this host. Decreases in dry matter contents of spearmint plants as well as concentrations of chemical elements were similar to those of peppermint. A deterrent interaction between the fungus Verticillium dahliae Kleb. and M. hapla was observed when peppermint and spearmint plants were inoculated with the two pathogens. Significantly (at the one percent level) less root galls were formed when V. dahliae was combined with different densities of M. hapla larvae. Also interaction between fungus and nematode caused longer incubation periods and less severe Verticillium wilt disease symptoms. Weight reductions due to such interaction were insignificant and the data indicate that peppermint was more susceptible to the pathogens than spearmint. It can be concluded that M. hapla can infect both peppermint and spearmint without showing obvious disease symptoms on aerial parts of plants. Therefore, infections can remain unnoticed particularly in spearmint which has more tolerance to the nematode Introduction of root-knot nematode infected planting stock to new planting sites or other fields spreads the parasite which will affect other agricultural crops. Also suppression of Verticillium wilt disease symptoms by the nematodes could complicate or delay early diagnosis and control of wilt disease.
Author: Brenda Valeska Ortiz Uribe Publisher: ISBN: Category : Cotton Languages : en Pages : 484
Book Description
Site-specific management (SSM) is a promising strategy for reducing yield losses caused by the southern root-knot nematode [Meloidogyne incognita (Kofoid & White) Chitwood] (RKN) across the U.S Cotton Belt. To address this opportunity, this dissertation addresses the analysis of the spatial variability of RKN and its spatial relationship to edaphic, terrain, and chemical field properties. Additionally, simulations of RKN damage on different cotton biomass components, through adaptations to the CROPGRO-Cotton growth model, were used to estimate the damage of RKN within zones with a high likelihood for high RKN population. The work was conducted in the Tifton-Vidalia Upland (TVU) ecoregion of the southeastern Coastal Plain. Data were collected from eleven producers' fields and one university-owned field used for a RKN long-term research project during 2005, 2006, and 2007. The fields were located in Colquitt, Tift, and Worth Counties of Georgia, USA. Two different approaches were used to identify field features related to the presence or absence of RKN: (i) geostatistical analyses (factorial kriging) to decompose the variability of RKN and soil properties into different spatial components allowing the computation of correlation coefficients for different spatial scales; and (ii) canonical correlation analyses (CCA) to determine which properties explained the greatest amount of variability in RKN population density. Areas at risk for different levels of RKN population were identified by indicator kriging and fuzzy clustering of canonical predictors derived from the CCA. The simulation of growth and yield of cotton plants infected with RKN was conducted by modifying the Cropping System Model (CSM)-CROPGRO-Cotton. The model was modified by coupling RKN population for removal of daily assimilate and decreasing root length per unit root weight as strategies to mimic RKN damage. This study showed that: (1) small patches with high RKN population were associated with the flat areas within a field and large patches were associated with low values of apparent soil electrical conductivity shallow (EC[subscript a-s], 0-30 cm depth) and deep (EC[subscript a-d], 0-90 cm depth); (2) areas at risk for RKN population above a threshold value can be delineated from a reduced number of RKN population samples and a dense data set of EC[subscript a-d]; (3) low values of EC[subscript a-d], slope (SL), and NDVI can be associated with areas having high population of RKN; (4) RKN management zones can be delineated from edaphic terrain properties; (5) EC[subscript a-s] and EC[subscript a-d] properties offer much more stable information than terrain properties to characterize areas with low and high risk for having presence of RKN population; (6) RKN parasitism reduces cotton growth and development and induces a delay in maturity; (7) the adaptations of the Cropping System Model (CSM)-CROPGRO-Cotton in DSSAT v4.0 by coupling RKN population density and reducing the root length per unit root weight allowed the simulation of growth and yield for the DP 458 BR cotton variety impacted by various levels of RKN population; and (8) the use CSM-CROPGRO-Cotton model to simulate the seed cotton weight for different management zones with various risk levels for RKN allowed the quantification of potential yield losses due to RKN parasitism. Overall, this research contributes to the knowledge of RKN population variability as a function of edaphic and terrain attributes within fields of south Georgia, and develops techniques for applying site specific management to the pervasive problem of the southern root-knot nematode.
Author: Amanda D. Howland
Author: Amanda D. Howland
Author: Isaac Lartey
Author: Kamal Kishore Chaudhary
Author: Rosa H. Manzanilla-López
Author: Hassan Eshtiaghi
Author: Charalambos Panayi
Author: Ioannis A. Anastasiadis
Author: Brenda Valeska Ortiz Uribe![Control of the Root-knot Nematode Meloidogyne Hapla [microform] PDF](https://books.google.com/books/content/images/frontcover/FCgaNAEACAAJ?fife=w80-h100)
Author: C. Panayi
Author: V. W. Saka