Author: Jordan Claire Turner ReddelPublisher:
ISBN:
Category :
Languages : en
Pages : 128
Book Description
Design, Synthesis, and Characterization of Novel Bifunctional Ligands for Incorporation Into a Fluorescent Polymer Sensor
Author: Jordan Claire Turner ReddelPublisher:
ISBN:
Category :
Languages : en
Pages : 128
Book Description
Design, Synthesis and Characterization of Conjugated Polymers as Fluorescent Sensors
Author: Yan ZhangPublisher:
ISBN:
Category : Fluorescent polymers
Languages : en
Pages : 258
Book Description
Design, Synthesis and Speciation of Bifunctional Ligands
Author: Synthesis and Characterization of Novel Fluorescent Sensors Based on Terpyridine, Benzoxazole and Benzothiazole Chromophores
Author: Xiaoman BiPublisher:
ISBN:
Category : Fluorescent probes
Languages : en
Pages : 173
Book Description
Over the past few decades, there has been significant interest in developing fluorescent probes, because they are useful tools for biological studies. As effective analytical techniques, fluorescent probes utilize distinct advantages offered by fluorescence detection in terms of sensitivity, selectivity, and fast response time. When fluorescent probes interact selectively with target molecules, ions or biological specimens, they can generate large optical responses. Since most ions or molecules, such as Zn2+, Ca2+, or pyrophosphate ion (PPi), are non-fluorescent, chemosensors having analyte binding-triggered fluorescence are appealing in many fields, like analytical chemistry, clinical biochemistry, medicine, and environmental science.This dissertation is devoted to the design, synthesis, and characterization of novel fluorescent sensors for Zn2+ and its associated applications. Chapter II of this dissertation presents several novel terpyridine-based fluorescent sensors with different substituents affecting the electronic and steric nature of the terpyridine (tpy) fluorophore. Sensors are designed to establish the correlation between sensor structure and its photophysical properties. Low temperature fluorescence is used to evaluate the essential role of intramolecular charge transfer (ICT) in zinc binding-induced fluorescence changes. The tpy molecular fragment has a relatively large [pi]-conjugated system which enables the potential [pi-pi] interaction between two tpy platforms and affects the fluorescence of tpy ligands. Chapter III introduces a dimeric tpy ligand containing two tpy fragments connected via a meta-phenylene unit. The detailed spectroscopic study shows that this ligand displays an attractive fluorescence turn-on, in sharp contrast to mono(tpy) ligand that shows fluorescence quenching upon binding Zn2+. The result suggests the existence of delicate structural influences on fluorescence of tpy derivatives.Chapter IV is devoted to 2-(2'-hydroxyphenyl)-1,3-benzoxazole (HBO) and 2-(2'-hydroxyphenyl)-1,3-benzothiazole (HBT) derivatives featured with a structural potential of excited-state intramolecular proton transfer (ESIPT). The study reveals additional information on the binding of HBO or HBT to metal cations, which aids the sensor design for Zn2+ and PPi detection. The molecular design aims to realize ESIPT process control upon complexation with an analyte. Chapter V is devoted to the synthesis of bis(HBO) derivatives which bind Zn2+ selectively and emit near-infrared (NIR) fluorescence as a consequence of metal ion binding-induced ESIPT turn-on. Preliminary cell stain experiment was conducted and indicated the potential biological applications.
Rational Design, Synthesis and Characterization of Response Selective Fluorescent Sensors
Author: Ruisong XuPublisher:
ISBN: 9780549032717
Category :
Languages : en
Pages : 185
Book Description
In this dissertation, we established a new approach assisted by computational chemistry to design fluorescent sensors. The approach is applicable to predict the behavior of a fluorophore-bridge-receptor sensor based on photoinduced electron transfer (PET). Our first designed rhodamine based pH sensor exhibits strong fluorescence under acidic conditions and very weak fluorescence under basic conditions, just as the computations predicted.
Molecular Design, Synthesis and Characterization of Novel Polymers for Microelectronics
Author: Shu YangDesign, Synthesis, and Characterization of Coordination Compounds and Coordination Polymers Based on 1,2,3-triazolate Ligands
Author: Shyam BiswasSynopses
Author: Design, Synthesis, and Characterization of Novel Hydrophilic Fluorene-based Derivatives for Bioimaging Applications
Author: Dao NguyenPublisher:
ISBN:
Category : Fluorescence microscopy
Languages : en
Pages : 242
Book Description
In this work, hydrophilic fluorene-based derivatives that contain ethylene oxide substituents, have been synthesized and characterized for potential use as new fluorophores for bioimaging applications and for fluorescence sensing of heavy metals. Symmetrical and unsymmetrical fluorene derivatives based on structural types of acceptor-[pi]-acceptor, acceptor-[pi]-donor, and donor-[pi]-donor were characterized by TGA, UV-vis absorption, fluorescence emission, lifetime, anisotropy, and two-photon absorption (2PA) cross section. They were found to possess high thermal stability, high photostability, high fluorescence quantum yields, and generally large two-photon absorption cross sections, making them quite suitable for new probes in single-photon absorption and two-photon absorption fluorescence microscopy imaging. Novel hydrophilic fluorene derivatives were synthesized from fluorene in multiple steps employing the metal-catalyzed Heck coupling reaction, the Stille reaction, the Sonogashira reaction, the Ullmann condensation reaction, and "click" chemistry. To increase the hydrophilicity of the new compounds, ethylene oxide substituents were utilized for to impart water solubility. An alternative alkylation methodology using ethyleneoxy tosylates was introduced for the synthesis of ethylene oxide-containing fluorene derivatives. Several of these hydrophilic derivatives were incubated into various cell lines as new probes for both conventional and two-photon absorption fluorescence bioimaging. These compounds were biocompatible, exhibiting low cytotoxicity as determined by cell viability studies, and displayed colocalization for selected cellular organelles. In addition, hydrophilic bis(1,2,3-triazolyl)fluorene derivatives were found to exhibit sensitive fluorescence responses in the presence of certain heavy metal, and were selective for sensing zinc and mercury over other a number of other metal ions relevant to living cells or other biological environments. The UV-vis absorption and fluorescence emission spectra of the complexes exhibited a blue-shifted absorption and emission for selective metal chelation upon binding to zinc and mercury(II) ions, resulting in an approximately two-fold enhanced fluorescence response. Fluorescence titration studies revealed that the complexes of 1:2 and 1:3 ligand to metal formed with binding constant values of 108 and 1014 for zinc and mercury ions, respectively. Finally, preliminary experiments were performed to explore the possibility of employing select hydrophilic fluorene-based derivatives in the synthesis of hydrophilic fluorescent gold nanoparticles. Although results are very preliminary, the aim is to use such materials for other biomedical applications, such as surface enhanced scattering resonance and noninvasive photothermal therapy to diagnose and to treat cancers. Thus, this research had led to the discovery of alternative methodologies for synthesis of hydrophilic fluorene derivatives by alkylation with alkyl tosylates and synthesis of hydrophilic fluorescent molecule capped gold nanoparticles. Furthermore, several novel hydrophilic fluorene-based derivatives were synthesized and characterized for their linear and nonlinear photophysical properties, and are now available for further examination of their bioimaging and sensing applications.
Author: