Densitometry in Thin Layer Chromatography PDF Download

Author: Joseph C. Touchstone
Publisher: John Wiley & Sons
ISBN:
Category : Science
Languages : en
Pages : 776

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Book Description
A first-of-its kind work covering instruments and methods for quantitation of materials separated on thin layer chromatography in situ by densitometry. Includes densitometer uses, the preparation of samples, and a step-by-step guide to the entire process. Provides a thorough discussion of the Kubelka-Munk Theory showing how it applies to densitometry of chromatograms. Also features a listing of available instrumentation with operating parameters, details of methodology for quantitation of many classes of compounds, and how-to-do-it and applications sections.

Author: Colin Poole
Publisher: Elsevier
ISBN: 0323983014
Category : Science
Languages : en
Pages : 692

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Book Description
Instrumental Thin-Layer Chromatography, Second Edition offers a comprehensive source of authoritative information on all aspects of instrumental thin-layer chromatography. The use of short, topic-focused chapters facilitates identifying information of immediate interest for familiar or emerging uses of thin-layer chromatography. The book gives those working in both academia and industry the opportunity to learn, refresh, or deepen their understanding of fundamental and instrumental aspects of thin-layer chromatography, as well as the tools to interpret and manage chromatographic data. The book serves as a practical consolidated guide to the selection of separation conditions and the use of auxiliary techniques. This fully updated new edition restores the contemporary character of the book for those involved in advancing the technology, analyzing data produced, or applying the technique to new application areas. Some chapters have been consolidated to make room for topics not covered in the first edition, reflecting general changes in the field of thin-layer chromatography, especially in effects-directed detection, convenient interfaces for advanced spectroscopic detection, and greater automation possibilities. This book is a valuable reference for anyone who needs to acquire fundamental and practical information to facilitate progress in research and management functions utilizing information acquired by thin-layer chromatography. - Features individual chapters written by recognized authoritative and visionary experts in the field - Provides an overview and focused treatment of a single topic - Provides tables and diagrams with commonly used data to facilitate practical work, comparison of results, and decision-making - Places modern developments in the research literature into a general context not always apparent to inexperienced users of the technique - Offers comprehensive updates to all chapters - Includes new chapters on instrument platforms, effects-directed detection, data analysis tools, small-scale and microfluidic planar separation systems, and applications to the separation of amino acids and peptides, the analysis of saccharides and lipids, and forensic analysis

Author: Bernd Spangenberg
Publisher: Springer Science & Business Media
ISBN: 364210729X
Category : Science
Languages : en
Pages : 399

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Book Description
Thin-layer chromatography (TLC) is widely used particularly for pharmaceutical and food analysis. While there are a number of books on the qualitative identification of chemical substances by TLC, the unique focus here is on quantitative analysis. The authors describe all steps of the analytical procedure, beginning with the basics and equipment for quantitative TLC followed by sample pretreatment and sample application, development and staining, scanning, and finally statistical and chemometric data evaluation and validation. An important feature is the coverage of effect-directed biological detection methods. Chapters are organized in a modular fashion facilitating the easy location of information about individual procedural steps.

Author: Monika Waksmundzka-Hajnos
Publisher: CRC Press
ISBN: 1420046780
Category : Science
Languages : en
Pages : 898

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Book Description
Thin layer chromatography (TLC) is increasingly used in the fields of plant chemistry, biochemistry, and molecular biology. Advantages such as speed, versatility, and low cost make it one of the leading techniques used for locating and analyzing bioactive components in plants. Thin Layer Chromatography in Phytochemistry is the first sourc

Author: Lukasz Komsta
Publisher: CRC Press
ISBN: 1466507152
Category : Medical
Languages : en
Pages : 1070

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Book Description
Used routinely in drug control laboratories, forensic laboratories, and as a research tool, thin layer chromatography (TLC) plays an important role in pharmaceutical drug analyses. It requires less complicated or expensive equipment than other techniques, and has the ability to be performed under field conditions. Filling the need for an up-to-date, complete reference, Thin Layer Chromatography in Drug Analysis covers the most important methods in pharmaceutical applications of TLC, namely, analysis of bulk drug material and pharmaceutical formulations, degradation studies, analysis of biological samples, optimization of the separation of drug classes, and lipophilicity estimation. The book is divided into two parts. Part I is devoted to general topics related to TLC in the context of drug analysis, including the chemical basis of TLC, sample pleparation, the optimization of layers and mobile phases, detection and quantification, analysis of ionic compounds, and separation and analysis of chiral substances. The text addresses the newest advances in TLC instrumentation, two-dimensional TLC, quantification by slit scanning densitometry and image analysis, statistical processing of data, and various detection and identification methods. It also describes the use of TLC for solving a key issue in the drug market—the presence of substandard and counterfeit pharmaceutical products. Part II provides an in-depth overview of a wide range of TLC applications for separation and analysis of particular drug groups. Each chapter contains an introduction about the structures and medicinal actions of the described substances and a literature review of their TLC analysis. A useful resource for chromatographers, pharmacists, analytical chemists, students, and R&D, clinical, and forensic laboratories, this book can be utilized as a manual, reference, and teaching source.

Author: ManMohan Srivastava
Publisher: Springer Science & Business Media
ISBN: 3642140254
Category : Science
Languages : en
Pages : 401

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Book Description
The present edited book is the presentation of 18 in-depth national and international contributions from eminent professors, scientists and instrumental chemists from educational institutes, research organizations and industries providing their views on their experience, handling, observation and research outputs on HPTLC, a multi-dimensional instrumentation. The book describes the recent advancements made on TLC which have revolutionized and transformed it into a modern instrumental technique HPTLC. The book addresses different chapters on HPTLC fundamentals: principle, theory, understanding; instrumentation: implementation, optimization, validation, automation and qualitative and quantitative analysis; applications: phytochemical analysis, biomedical analysis, herbal drug quantification, analytical analysis, finger print analysis and potential for hyphenation: HPTLC future to combinatorial approach, HPTLC-MS, HPTLC-FTIR and HPTLC-Scanning Diode Laser. The chapters in the book have been designed in such away that the reader follows each step of the HPTLC in logical order.

Author: Eike Reich
Publisher: Thieme
ISBN: 1604065273
Category : Medical
Languages : en
Pages : 292

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Book Description
High-Performance Thin-Layer Chromatography for the Analysis of Medicinal Plants presents the theoretical and technical information needed to perform reliable and reproducible high-performance thin-layer chromatography (HPTLC) to establish the identity, purity, quality, and stability of raw materials, extracts, and finished botanical products. The text provides a complete overview of the technique and common applications of HPTLC in herbal analysis. It will help the analyst answer questions such as: Am I paying for a high-quality material, but getting a cheap adulterant? Is this raw material worth its price? Does this product comply with the claim on its label? Has the composition of this product changed after being on the shelf for more than a year? Practical examples provided by renowned experts help the reader gain a firm understanding of HPTLC methodologies. More than 300 full-color illustrations aid comprehension of complex concepts, and easy-to-reference text boxes provide summaries of key information.This book is essential for analysts, quality assurance professionals, and regulators seeking a comprehensive text on how to use HPTLC to determine whether botanicals comply with current, good manufacturing practices. It will also benefit students in pharmacognosy, phytopharmacy, pharmaceutical biology, and analytical chemistry programs.

Author: Teresa Kowalska
Publisher: CRC Press
ISBN: 1498705898
Category : Medical
Languages : en
Pages : 396

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Book Description
Planar Chromatography-Mass Spectrometry focuses on a relatively new approach to chemical analysis in general, and to separation science in particular. It is the first book to systemically cover the theoretical background, techniques, instrumentation, and practical applications of planar chromatography-mass spectrometry as a hyphenated tool of analy

Author: I. Mikšík
Publisher: Elsevier
ISBN: 0080858686
Category : Science
Languages : en
Pages : 1149

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Book Description
This book deals with chromatographic and electrophoretic methods applied for the separation (quantitation and identification) of biologically relevant compounds. It is assumed that the potential reader is familiar with the basics of chromatographic and electromigration methods. Individual separation modes are dealt with to an extent which follows their applicability for biomedical purposes: liquid chromatography and electromigration methods are therefore highlighted.Each chapter is completed with a list of recent literature covering the 1987-1997 period, which can be used for further guidance of the reader in his/her own field. The chapters have been written by specialists in a particular area and with an emphasis on applications to the biomedical field. This implies that theoretical and instrumental aspects are kept to a minimum which allows the reader to understand the text. Considerable attention is paid to method selection, detection and derivatization procedures and troubleshooting. The majority of examples given represent the analyses of typical naturally-occurring mixtures. Adequate attention is paid to the role of the biological matrix and sample pretreatment, and special attention is given to forensic, toxicological and clinical applications. The book is completed with an extensive Index of Compounds Separated.

Author: Bernard Fried
Publisher: CRC Press
ISBN: 9780203910214
Category : Science
Languages : en
Pages : 516

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Book Description
The fourth edition of this work emphasizes the general practices and instrumentation involving TLC and HPTLC, as well as their applications based on compound types, while providing an understanding of the underlying theory necessary for optimizing these techniques. The book details up-to-date qualitative and quantitative densitometric experiments on organic dyes, lipids, antibiotics, pharmaceuticals, organic acids, insecticides, and more.