Chromatographie Liquide de Haute Performance en Chimie Des Protéines PDF Download

Author: Henri Wajcman
Publisher:
ISBN:
Category : Amino acid sequence
Languages : en
Pages : 232

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Author: M. Kastner
Publisher: Elsevier
ISBN: 9780444502117
Category : Science
Languages : en
Pages : 978

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Book Description
Protein Liquid Chromatography is a handbook-style guide to liquid chromatography as a tool for isolating and purifying proteins, consisting of 25 individual chapters divided into three parts: Part A covers commonly-used, classic modes of chromatography such as ion-exchange, size-exclusion, and reversed-phase; Part B deals with various target protein classes such as membrane proteins, recombinant proteins, and glycoproteins; and Part C looks at various miscellaneous related topics, including coupling reaction, buffer solution additives, and software. The text as a whole can be viewed as a systematic survey of available methods and how best to use them, but also attempts to provide an exhaustive coverage of each facet. How to solve a specific problem using a chosen method is the overall essence of the volume. The principle philosophy of this compilation is that practical application is everything; therefore, both classical and modern methods are presented in detail, with examples involving conventional, medium- and high-pressure techniques. Over-exposure to history, concept, and theory has deliberately been avoided. The reader will find a wealth of tips and tricks from users for users, including advice on the advantages and disadvantages of each method. Easy-to-read sections on "Getting started now" and "Where to go from here" attempt to provide hands-on, fool-proof detailed practical procedures with complete and even standard model runs for any scientist or technician at work in this area.

Author: Michel Anselme
Publisher:
ISBN:
Category :
Languages : fr
Pages : 150

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Author: Milton Hearn
Publisher: Elsevier
ISBN: 032315770X
Category : Science
Languages : en
Pages : 284

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Book Description
High-Performance Liquid Chromatography of Proteins and Peptides contains the proceedings of the first International Symposium on High-Performance Liquid Chromatography of Proteins and Peptides, held in Washington, D.C., on November 16-17, 1981. The symposium focused on the use of high-performance liquid chromatography (HPLC) in the analysis, characterization, and isolation of peptides and proteins and encompassed six sessions covering size exclusion, ion exchange, and reversed phase chromatography, as well as the use of high-performance liquid chromatography (HPLC) in protein structural studies and peptide isolation. This book is comprised of 28 chapters and begins with a discussion on the status of high-performance ion-exchange chromatography of proteins, followed by an analysis of peptic fragmentation of human immunoglobulin G using HPLC. The physicochemical basis of peptide retention with chemically bonded hydrocarbonaceous silicas and the isolation of biologically active peptides from tissue extracts are also examined. Subsequent chapters explore some additional applications of HPLC, such as cord blood screening for hemoglobin disorders; purification of commercial trypsin and chymotrypsin; characterization of human alcohol dehydrogenase isoenzymes; and structural studies of neurophysins, photolabeled derivatives, and biosynthetic precursors. This monograph should be of value to students and researchers interested in the use of HPLC to study proteins and peptides.

Author: ARMELLE.. DE KERMOYSAN
Publisher:
ISBN:
Category :
Languages : fr
Pages :

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Author: National Library of Medicine (U.S.)
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 1712

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First multi-year cumulation covers six years: 1965-70.

Author: Isabelle Gaillard
Publisher:
ISBN:
Category :
Languages : fr
Pages :

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DEPUIS LA MISE AU POINT DES TECHNIQUES DE FORMATION DES LIGNEES D'HYBRIDOMES, DES EFFORTS IMPORTANTS SONT FOURNIS POUR AMELIORER LA PRODUCTIVITE DES ANTICORPS MONOCLONAUX ET POUR MIEUX COMPRENDRE LE METABOLISME CELLULAIRE. LES METHODES ANALYTIQUES JOUENT UN ROLE IMPORTANT DANS LE CONTROLE DES PROCEDES EN PERMETTANT LA DETERMINATION QUANTITATIVE ET QUALITATIVE DE PLUSIEURS PARAMETRES ET VARIABLES. L'OBJECTIF GENERAL DE CETTE ETUDE CONSISTE A CONTRIBUER A L'AMELIORATION DE LA MAITRISE DES BIOPROCEDES EN PROPOSANT UNE METHODE ANALYTIQUE CAPABLE DE QUANTIFIER LES PROTEINES PRESENTES DANS LES CULTURES D'HYBRIDOMES. AFIN D'ATTEINDRE CET OBJECTIF, LES POTENTIALITES DE L'HPLC ONT ETE TESTEES. LES PROTEINES CONSIDEREES DANS CETTE ETUDE SONT D'UNE PART, LES COMPOSES PROTEIQUES PROPRES AU MILIEU DE CULTURE ET D'AUTRE PART, LES ANTICORPS MONOCLONAUX SECRETES PAR LES CELLULES. LA PREMIERE PARTIE DE CETTE ETUDE PRESENTE L'OPTIMISATION DES CONDITIONS OPERATOIRES DE LA METHODE CHROMATOGRAPHIQUE. LES DEUX MODES CHROMATOGRAPHIQUES MIS A PROFIT SONT L'HPLC D'AFFINITE ET LA RP-HPLC. UNE ETUDE COMPARATIVE DES PERFORMANCES DE LA METHODE PAR HPLC ET DU TEST ELISA EST EXPOSEE DANS UNE SECONDE PARTIE. LA TROISIEME PARTIE CONCERNE L'APPLICATION DE LA METHODE CHROMATOGRAPHIQUE AUX SUIVIS DES PROTEINES DE CULTURES CELLULAIRES D'HYBRIDOMES. ENFIN, LA REALISATION COUPLAGE AUTOMATISE REACTEUR-ECHANTILLONNEUR-HPLC ET SON APPLICATION A LA DETERMINATION EN LIGNE DE LA CONCENTRATION DES IGG D'UNE CULTURE DISCONTINUE, CONSTITUENT LA DERNIERE PARTIE DE CE TRAVAIL

Author: Giorgio Carta
Publisher: John Wiley & Sons
ISBN: 352734666X
Category : Science
Languages : en
Pages : 440

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Book Description
An all-in-one practical guide on how to efficiently use chromatographic separation methods Based on a training course that teaches the theoretical as well as practical aspects of protein bioseparation to bioprocess professionals, this fully updated and revised new edition offers comprehensive coverage of continuous chromatography and provides readers with many relevant examples from the biopharmaceutical industry. Divided into two large parts, Protein Chromatography: Process Development and Scale-Up, Second Edition presents all the necessary knowledge for effective process development in chromatographic bioseparation, both on small and large scale. The first part introduces chromatographic theory, including process design principles, to enable the reader to rationalize the set-up of a bioseparation process. The second part illustrates by way of case studies and sample protocols how the theory learned in the first part may be applied to real-life problems. Chapters look at: Downstream Processing of Biotechnology Products; Chromatography Media; Laboratory and Process Columns and Equipment; Adsorption Equilibrium; Rate Processes; and Dynamics of Chromatography Columns. The book closes with chapters on: Effects of Dispersion and Rate Processes on Column Performance; Gradient Elution Chromatography; and Chromatographic Column Design and Optimization. -Presents the most pertinent examples from the biopharmaceutical industry, including monoclonal antibodies -Provides an overview of the field along with design tools and examples illustrating the advantages of continuous processing in biopharmaceutical productions -Focuses on process development and large-scale bioseparation tasks, making it an ideal guide for the professional bioengineer in the biotech and pharma industries -Offers field-tested information based on decades of training courses for biotech and chemical engineers in Europe and the U.S. Protein Chromatography: Process Development and Scale-Up, Second Edition will appeal to biotechnologists, analytical chemists, chromatographers, chemical engineers, pharmaceutical industry, biotechnological industry, and biochemists.

Author: National Library of Medicine (U.S.)
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 1728

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Author: Arne Staby
Publisher: John Wiley & Sons
ISBN: 1119031176
Category : Technology & Engineering
Languages : en
Pages : 608

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Book Description
Preparative Chromatography for Separation of Proteins addresses a wide range of modeling, techniques, strategies, and case studies of industrial separation of proteins and peptides. • Covers broad aspects of preparative chromatography with a unique combination of academic and industrial perspectives • Presents Combines modeling with compliantce useing of Quality-by-Design (QbD) approaches including modeling • Features a variety of chromatographic case studies not readily accessible to the general public • Represents an essential reference resource for academic, industrial, and pharmaceutical researchers