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Characterization of Plant Viruses

Characterization of Plant Viruses PDF Author: Alangar Ishwara Bhat
Publisher: Humana
ISBN: 9781071603369
Category : Science
Languages : en
Pages : 552

Book Description
This book provides detailed information on methodologies used in biological, serological and nucleic acid based assays for the detection, diagnosis and management of plant viruses. The content is divided into six main parts, the first of which presents techniques used in the biological characterization and transmission of viruses, while Part II covers purification and techniques concerning the physico-chemical properties of viruses. In turn, Part III focuses on in vitro expression, production of polyclonal and monoclonal antibodies; and on various serological assays including precipitin tests, ELISA, blot immunoassays, immunosorbent electron microscopy and lateral flow immunoassays. Part IV addresses the isolation of DNA and RNA from plants and nucleic acid based assays such as dot-blot, polymerase chain reaction, real-time PCR, loop-mediated isothermal amplification, rolling circle amplification, recombinase polymerase amplification, and next-generation sequencing approaches. Part V discusses cloning, sequencing, sequence analysis and the production of infectious clones, while the last part (Part VI) provides biotechnological approaches for the management of viruses. Given its scope, the book will be a valuable resource for every laboratory, student and teacher, and for everyone interested in plant virology, plant pathology, plant biology and molecular biology, offering them a practical manual on various aspects of plant viruses.

Characterization of Plant Viruses

Characterization of Plant Viruses PDF Author: Alangar Ishwara Bhat
Publisher: Humana
ISBN: 9781071603369
Category : Science
Languages : en
Pages : 552

Book Description
This book provides detailed information on methodologies used in biological, serological and nucleic acid based assays for the detection, diagnosis and management of plant viruses. The content is divided into six main parts, the first of which presents techniques used in the biological characterization and transmission of viruses, while Part II covers purification and techniques concerning the physico-chemical properties of viruses. In turn, Part III focuses on in vitro expression, production of polyclonal and monoclonal antibodies; and on various serological assays including precipitin tests, ELISA, blot immunoassays, immunosorbent electron microscopy and lateral flow immunoassays. Part IV addresses the isolation of DNA and RNA from plants and nucleic acid based assays such as dot-blot, polymerase chain reaction, real-time PCR, loop-mediated isothermal amplification, rolling circle amplification, recombinase polymerase amplification, and next-generation sequencing approaches. Part V discusses cloning, sequencing, sequence analysis and the production of infectious clones, while the last part (Part VI) provides biotechnological approaches for the management of viruses. Given its scope, the book will be a valuable resource for every laboratory, student and teacher, and for everyone interested in plant virology, plant pathology, plant biology and molecular biology, offering them a practical manual on various aspects of plant viruses.

Virus Diseases of Ornamental Plants

Virus Diseases of Ornamental Plants PDF Author: S. K. Raj
Publisher: Springer Nature
ISBN: 9811639191
Category : Science
Languages : en
Pages : 402

Book Description
This edited book elucidates the evolution of plant virus, genomic structure, diversity, plant-virus interaction, subcellular movement etc. The book reviews the biological machineries which allow the emergence of virus populations adapted by plant. The main objective of this book is the demonstration of a clear synergistic effect of plant viruses, an effect that was unexpectedly as important as applied alone. Ornamental plants are very popular and economically important worldwide. The international market of ornamentals is constantly expanding. Viruses and viroids can significantly reduce both decorative value and quality of propagated material of ornamentals. Due to the wide range of ornamental plant species and cultivars and their wide geographical distribution, the diversity of viruses that infect them is also high. The new emerging viruses are the causal agent for the economic loss of many important ornamental plants. Therefore, this book also adds value to current knowledge of virus stress response in ornamental plants and will provide the groundwork necessary for building future strategies for product enhancement. This book is of interest to teachers, researchers, capacity builders and policymakers. It can serve as additional reading material for undergraduate and graduate students of virology, agriculture and plant sciences.

Applied Plant Virology

Applied Plant Virology PDF Author: L. P. Awasthi
Publisher: Academic Press
ISBN: 0128220538
Category : Science
Languages : en
Pages : 851

Book Description
Applied Plant Virology: Advances, Detection, and Antiviral Strategies provides an overview on recent developments and applications in the field of plant virology. The book begins with an introduction to important advances in plant virology, but then covers topics including techniques for assay detection and the diagnosis of plant viruses, the purification, isolation and characterization of plant viruses, the architecture of plant viruses, the replication of plant viruses, the physiology of virus-infected hosts, vectors of plant viruses, and the nomenclature and classification of plants. The book also discusses defense strategies by utilizing antiviral agents and management strategies of virus and viroid diseases. With contributions from an international collection of experts, this book presents a practical resource for plant virologists, plant pathologists, horticulturalists, agronomists, biotechnologists, academics and researchers interested in up-to-date technologies and information that advance the field of plant virology. - Covers the detection, control and management of plant viruses - Discusses antiviral strategies, along with mechanisms of systemic induced resistance to enhance the defense of plants against viruses - Provides contributory chapters from expert plant virologists from different parts of the world

Plant Virus Evolution

Plant Virus Evolution PDF Author: Marilyn J. Roossinck
Publisher: Springer Science & Business Media
ISBN: 3540757635
Category : Science
Languages : en
Pages : 227

Book Description
This book provides a comprehensive look at the field of plant virus evolution. It is the first book ever published on the topic. Individual chapters, written by experts in the field, cover plant virus ecology, emerging viruses, plant viruses that integrate into the host genome, population biology, evolutionary mechanisms and appropriate methods for analysis. It covers RNA viruses, DNA viruses, pararetroviruses and viroids, and presents a number of thought-provoking ideas.

Plant Virus Epidemiology

Plant Virus Epidemiology PDF Author:
Publisher: Elsevier
ISBN: 0080466370
Category : Science
Languages : en
Pages : 556

Book Description
Published since 1953, Advances in Virus Research covers a diverse range of in-depth reviews providing a valuable overview of the current field of virology. In 2004, the Institute for Scientific Information released figures showing that the series has an Impact Factor of 2.576, with a half-life of 7.1 years, placing it 11th in the highly competitive category of Virology.* Edited by an experienced plant pathologist who has over 50 years experience in plant virus epidemiology* Covers topics such as Evolutionary epidemiology of plant virus disease, The control of tropical plant virus diseases, and Control of plant virus diseases* A valuable resource for students and researchers alike

Recent Advances in the Diagnosis and Management of Plant Diseases

Recent Advances in the Diagnosis and Management of Plant Diseases PDF Author: L.P. Awasthi
Publisher: Springer
ISBN: 8132225716
Category : Technology & Engineering
Languages : en
Pages : 302

Book Description
This book is a compilation of the most challenging and significant chapters on the diagnosis and management of important bacterial, fungal, viral, viroid, phytoplasma, non parasitic diseases and various physiological disorders, in various crops. The chapters have been contributed by eminent plant pathologists, having wide experience of teaching and research on various crops with different types of diseases, which cause great economic losses. The book would be very useful for students, teachers and researchers of plant pathology. This book highlights recent advances made in the development of new types of resistance in host plants and alternative strategies for managing plant diseases to improve food quality and reduce the negative public health impact associated with plant diseases. Having entered into 21st century advancements in the Diagnosis of Plant Pathogens and Plant Disease Management need to be closely examined and adequately applied, so that newer challenges facing plant pathology could be adequately addressed in attaining food security for the growing population. Substantial advancements have been made in terms of expanding knowledge base of the biology of plant-microbial interactions, disease management strategies and application and practice of Plant Pathology. Application of molecular biology in Plant Pathology has greatly improved our ability to detect plant pathogens and in increasing our understanding, their ecology and epidemiology. Similarly, new technologies and resources have been evolved for the development of sustainable crop protection systems by different control strategies against various pests and pathogens that are important components of the integrated pest management programme. Natural products and chemical compounds discovered as a result of basic research and molecular mechanisms of pathogenesis have led to the development of “biorational” pesticides. Biological control has been found to be the most significant approach to plant health management during the twentieth century and promises using modern biotechnology, to be even more significant in the twenty-first century.

Seed-borne plant virus diseases

Seed-borne plant virus diseases PDF Author: K. Subramanya Sastry
Publisher: Springer Science & Business Media
ISBN: 8132208137
Category : Medical
Languages : en
Pages : 343

Book Description
Seeds provide an efficient means in disseminating plant virus and viroid diseases. The success of modern agriculture depends on pathogen free seed with high yielding character and in turn disease management. There is a serious scientific concern about the transmission of plant viruses sexually through seed and asexually through plant propagules. The present book provides the latest information along with the total list of seed transmitted virus and viroid diseases at global level including, the yield losses, diagnostic techniques, mechanism of seed transmission, epidemiology and virus disease management aspects. Additional information is also provided on the transmission of plant virus and virus-like diseases through vegetative propagules. It is also well known that seed transmitted viruses are introduced into new countries and continents during large-scale traffic movements through infected germplasm and plant propogules. The latest diagnostic molecular techniques in different virus-host combinations along with disease management measures have been included. The book shall be a good reference source and also a text book to the research scientists, teachers, students of plant pathology, agriculture, horticulture, life sciences, green house managers, professional entrepreneurs, persons involved in quarantines and seed companies. This book has several important features of seed transmitted virus diseases and is a good informative source and thus deserves a place in almost all university libraries, seed companies and research organizations.

Biotechnology and Plant Disease Management

Biotechnology and Plant Disease Management PDF Author: Zamir K. Punja
Publisher: CABI
ISBN: 1845933109
Category : Science
Languages : en
Pages : 591

Book Description
As agricultural production increases to meet the demands of a growing world population, so has the pace of biotechnology research to combat plant disease. Diseases can be caused by a variety of complex plant pathogens including fungi, bacteria, viruses and nematodes, and their management requires the use of techniques in transgenic technology, biochemistry and genetics. While texts exist on specific pathogens or management practices, a comprehensive review is needed of recent developments in modern techniques and the understanding of how pathogens cause disease. This collection of studies discusses the key approaches to managing each group of pathogens within the context of recent developments in biotechnology. Broad themes include microbe-plant interactions, molecular diagnostics of plant pathogens and enhancing the resistance of plants.

Plant Virus and Viroid Diseases in the Tropics

Plant Virus and Viroid Diseases in the Tropics PDF Author: K. Subramanya Sastry
Publisher: Springer Science & Business Media
ISBN: 9400778201
Category : Science
Languages : en
Pages : 511

Book Description
Around the globe, besides fungal and bacterial diseases, both virus and viroid diseases have acquired greater importance in the realm of plant pathology and call for effective management measures as they are responsible for heavy yield losses and are a matter of vital importance and concern to farmers, horticulturists, gardeners and foresters. Understanding disease epidemiology is of vital importance for formulating viable disease management practices in a given agro-ecosystem. The development and progress of plant disease epidemics are variable from region to region. Epidemiology is not a static process, but rather a dynamic course that varies with a change in the ecology, host, vector and virus systems.

Molecular Methods in Plant Disease Diagnostics

Molecular Methods in Plant Disease Diagnostics PDF Author: Neil Boonham
Publisher: CABI
ISBN: 1780641478
Category : Science
Languages : en
Pages : 214

Book Description
Using molecular methods for plant disease diagnosis provides diagnosticians with a number of advantages over more traditional methods. They can allow the identification of morphologically similar species, for example, or the detection of infection prior to symptom formation. Not only can molecular tools help by increasing the efficacy, accuracy and speed of diagnosis; their common technological basis provides further benefits, especially where resources are limited and traditional skills are hard to sustain. This book provides protocols for nucleic acid-based methods currently applied to plant pathogen detection and identification. It takes the practitioner through the full range of molecular diagnostic and detection methods and, as these generic techniques are appropriate for use on any target with minimal modification, also provides a useful resource for students of plant pathology and plant pathologists. Beginning with the background and future directions of the science, it then addresses DNA barcoding, microarrays, polymerase chain reactions (PCR), quality assurance and more, forming a complete reference on the subject.