Author: William James Gordon-KammPublisher:
ISBN:
Category : Cell membranes
Languages : en
Pages : 640
Book Description
Changes in Membrane Morphology Associated with Two Forms of Freezing Injury in Isolated Rye Protoplasts (Secale Cereale L. Cv. Puma)
Author: William James Gordon-KammPublisher:
ISBN:
Category : Cell membranes
Languages : en
Pages : 640
Book Description
The Role of Soluble Sugars During Dehydrative Stress in Plants
Author: Karen Lee KosterComprehensive Dissertation Index
Author: Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 798
Book Description
American Doctoral Dissertations
Author: Publisher:
ISBN:
Category : Dissertation abstracts
Languages : en
Pages : 696
Book Description
Proceedings of the National Academy of Sciences of the United States of America
Author: Proceedings of the ... International Conference on Electron Microscopy
Author: Publisher:
ISBN:
Category : Electron microscopes
Languages : en
Pages : 932
Book Description
Effects of Freezing and Cold Acclimation on the Plasma Membrane of Isolated Protoplasts
Author: Publisher:
ISBN:
Category :
Languages : en
Pages : 7
Book Description
Our goal is to provide a mechanistic understanding of the cellular and molecular aspects of freezing injury and cold acclimation from a perspective of the structural and functional integrity of the plasma membrane -- the primary site of freezing injury in winter cereals. We have utilized protoplasts isolated from leaves of winter rye (Secale cereale L. cv Puma) to study the cryobehavior of the plasma membrane during a freeze/thaw cycle. The focus of our current studies is on lesions in the plasma membrane that result from severe freeze-induced dehydration and result in the alteration of the semipermeable characteristics of the plasma membrane so that the protoplasts are osmotically unresponsive. In protoplasts isolated from non-acclimated rye leaves (NA protoplasts), injury is associated with the formation of aparticulate domains in the plasma membrane, aparticulate lamellae subtending the plasma membrane, and lamellar-to-hexagonal II phase transitions in the plasma membrane and the subtending lamellae. However, lamellar-to-hexagonal II phase transitions are not observed following severe dehydration of protoplasts isolated from cold-acclimated rye leaves (ACC protoplasts). Rather, injury is associated with the fracture-jump lesion, '' which, in freeze-fracture electron microscopy studies, is manifested as localized deviations in the fracture face of the plasma membrane. The fracture plane jumps'' from the plasma membrane to either subtending aparticulate lamellae or aparticulate regions of various endomembranes (predominantly chloroplast envelopes) that are in close apposition with the plasma membrane.
Electron Microscopy
Author: The Plant Plasma Membrane
Author: Christer LarssonPublisher: Springer Science & Business Media
ISBN: 3642745229
Category : Science
Languages : en
Pages : 427
Book Description
The plasma membrane forms the living barrier between the cell and its surroundings. For this reason it has a wide range of important functions related to the regulation of the composition of the cell interior and to com munication with the cell exterior. The plasma membrane has therefore attracted a lot of research interest. Until the early 1970's it was only pos sible to study the plasma membrane in situ, its structure e. g. by electron microscopy and its function e. g. by uptake of radioactively labeled com pounds into the intact cell or tissue. The first isolation of plant protoplasts by enzymatic digestion of the cell wall in the early 1970's was an important step forward in that it provided direct access to the outer surface of the plasma membrane. More importantly, T. K. Hodges and R. J. Leonard in 1972 published the description of a method by which a fraction enriched in plasma membranes could be isolated from plant tissues using sucrose gradient centrifugation. As a result, the 1970's saw a leap forward in our understanding of the structurc and function of the plasma membrane. In 1981, S. Widell and C. Larsson published the first of a series of papers in which plasma membrane vesicles of high yield and purity were isolated from a wide range of plant tissues using aqueous polymer two-phase parti tioning.
Author: