Cell separation : methods and selected applications. 1 (1982) PDF Download

Author: Thomas G. Pretlow
Publisher:
ISBN:
Category : Cell separation
Languages : en
Pages : 330

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Author: Thomas G. Pretlow
Publisher: Academic Press
ISBN: 1483219380
Category : Science
Languages : en
Pages : 343

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Book Description
Cell Separation: Methods and Selected Applications is a compendium of articles on the design and/or application of methods for the separation of cells. This volume presents contributions on relatively finite subjects on cell separation. It covers topics on cell separation such as methods for obtaining cells in suspension from animal tissues; some of the kinds of data that are helpful in the description of cell purifications; and separation of host cells infiltrating tumors and allografts by velocity sedimentation at unit gravity. The separation of different kinds of nucleated cells from blood by centrifugal elutriation; a new approach to the separation of cells at unit gravity; and the isolation and culture of homogeneous populations of glomerular cell types are elucidated as well. Experimental oncologists, hematologists, immunologists, cell biologists, endocrinologists, and others who are not already expert in the use of methods for cell separation will find the book highly useful.

Author: Diether Recktenwald
Publisher: CRC Press
ISBN: 1482273640
Category : Medical
Languages : en
Pages : 352

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Book Description
"Offers complete coverage and assessment of cell separation technologies for analytical and preparative isolations of biological cell populations-demonstrating how to select and devise optimal sorting strategies for applications in biochemistry, immunology, cell and molecular biology, and clinical research. "

Author: A. M. Cheret
Publisher:
ISBN:
Category : Cell separation
Languages : en
Pages : 400

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Author: Theresa P Pretlow
Publisher:
ISBN: 9780125645034
Category :
Languages : en
Pages :

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Author: Dhinakar S. Kompala
Publisher:
ISBN:
Category : Language Arts & Disciplines
Languages : en
Pages : 320

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Book Description
This new volume provides theoretical and practical information on the state of the science of cell separation, including applications to biotechnology. Presentations from the world's leading authorities cover the four principal modern methods: flow sorting and optical methods; sedimentation and flow fractionation; affinity adsorption and extraction methods; and electrophoresis and magnetic methods. An introductory chapter describes the rationale for separating living cells, the parameters within which separation methods are evaluated, and some physical characteristics of each of the methods reported in the volume. Readers will be able to choose, evaluate, and in some cases, build cell separation procedures and equipment for their own purposes based on the contents of the book, which also covers many applications of each method.

Author: P.T. Sharpe
Publisher: Elsevier
ISBN: 0080858872
Category : Science
Languages : en
Pages : 287

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Book Description
Methods of Cell Separation brings to the attention of researchers at all levels the variety of methods available for separating viable populations of cells. Methods are grouped into 3 categories based on the criteria of separation, namely; size or density; non-specific surface properties; and specific surface properties. The principle of each method is described together with general and, where possible, specific protocols for conducting cell separation experiments.A central theme of the book is the separation of populations of blood lymphocytes which is used as an example for each method.Methods of Cell Separation is distinguished by three powerful assets: descriptions of the majority of cell separation methods currently being used; details of the experimental procedures involved in each method; and comparisons of the different methods for separating cell populations with particular reference to blood lymphocytes.An excellent addition to a distinguished series, and extremely useful as a laboratory manual.

Author: Thomas G. Pretlow
Publisher: Academic Press
ISBN: 1483219399
Category : Science
Languages : en
Pages : 344

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Book Description
Cell Separation: Methods and Selected Applications, Volume 2 provides information pertinent to the design and application of methods for the separation of cells. This book covers a variety of topics, including thyroid structure and function, procurement of eosinophils, elutriation, stromal cells, isolation of epithelial cells, and separation of cells. Organized into 15 chapters, this volume begins with an overview of the structure of thyroid parenchyma and the properties of endocrine parafollicular cells. This text then summarizes the various aspects of the separation methodology to assist investigators with the use of elutriation. Other chapters describe the methods that are useful for the separation of stromal cells from tumors. This book discusses as well the types of methods that are available for the purification of cells from tumors. The final chapter deals with the biological significance of the erythroblastic nest. This book is a valuable resource for cell biologists, experimental oncologists, hematologists, immunologists, and endocrinologists.

Author: Derek Fisher
Publisher: OUP Oxford
ISBN: 0191565555
Category : Science
Languages : en
Pages : 290

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Book Description
Techniques for separating cells are needed in many areas of cell biology. This book presents modern methods from the laboratories of experts in the field, and includes tested, reproducible protocols, hints and tips for success, and troubleshooting suggestions. It will be invaluable to a wide range of cell biologists.

Author: Thomas Laurell
Publisher: Royal Society of Chemistry
ISBN: 1849737061
Category : Technology & Engineering
Languages : en
Pages : 593

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Book Description
The manipulation of cells and microparticles within microfluidic systems using external forces is valuable for many microscale analytical and bioanalytical applications. Acoustofluidics is the ultrasound-based external forcing of microparticles with microfluidic systems. It has gained much interest because it allows for the simple label-free separation of microparticles based on their mechanical properties without affecting the microparticles themselves. Microscale Acoustofluidics provides an introduction to the field providing the background to the fundamental physics including chapters on governing equations in microfluidics and perturbation theory and ultrasound resonances, acoustic radiation force on small particles, continuum mechanics for ultrasonic particle manipulation, and piezoelectricity and application to the excitation of acoustic fields for ultrasonic particle manipulation. The book also provides information on the design and characterization of ultrasonic particle manipulation devices as well as applications in acoustic trapping and immunoassays. Written by leading experts in the field, the book will appeal to postgraduate students and researchers interested in microfluidics and lab-on-a-chip applications.