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Author: Zhixiang Wang Publisher: Springer Nature ISBN: 1071627368 Category : Science Languages : en Pages : 248
Book Description
This volume covers a broad range of cell types including cultured cell lines, primary cells, and various unicellular organisms such as fission yeast, budding yeast, parasite Leishmania amazonensis, and parasite Trypanosoma brucei. The chapters in this book are organized into four parts. Part One looks at a general overview of cell cycle control and synchronization. Part Two discusses techniques to synchronize mammalian cells to various cell cycle phases including mitotic sub-phases. Part Three covers synchronization of unicellular organisms and Part Four analyzes cell cycle progression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Cell-Cycle Synchronization: Methods and Protocols is a valuable resource for both novice and expert scientists in this developing field.
Author: Zhixiang Wang Publisher: Springer Nature ISBN: 1071627368 Category : Science Languages : en Pages : 248
Book Description
This volume covers a broad range of cell types including cultured cell lines, primary cells, and various unicellular organisms such as fission yeast, budding yeast, parasite Leishmania amazonensis, and parasite Trypanosoma brucei. The chapters in this book are organized into four parts. Part One looks at a general overview of cell cycle control and synchronization. Part Two discusses techniques to synchronize mammalian cells to various cell cycle phases including mitotic sub-phases. Part Three covers synchronization of unicellular organisms and Part Four analyzes cell cycle progression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Cell-Cycle Synchronization: Methods and Protocols is a valuable resource for both novice and expert scientists in this developing field.
Author: Amanda S. Coutts Publisher: Humana ISBN: 9781493929566 Category : Science Languages : en Pages : 0
Book Description
This volume brings together a unique collection of protocols that cover standard, novel, and specialized techniques. Cell Cycle Oscillators: Methods and Protocols guides readers through recent progress in the field from both holistic and reductionist perspectives, providing the latest developments in molecular biology techniques, biochemistry, and computational analysis used for studying oscillatory networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell Cycle Oscillators: Methods and Protocols will serve as an invaluable reference to gain further insight into the complex and incompletely understood processes that are involved in the cell cycle and its regulation by oscillatory networks.
Author: Ivan L. Cameron Publisher: Academic Press ISBN: 1483268977 Category : Science Languages : en Pages : 409
Book Description
Cell Synchrony: Studies in Biosynthetic Regulation focuses on the processes, principles, applications, methodologies, and approaches involved in biosynthetic regulation, including chromosome replication, cellular biosynthesis, and enzyme synthesis. The selection first takes a look at the theory of synchronous cultures, synchronous replication of the chromosome in Bacillus subtilis, and chromosome replication in Escherichia coli. Discussions focus on attempts to characterize the physical state of replicating DNA, cell division and the regulation of chromosome replication, regulation of sequential chromosome synthesis, synchrony of chromosome replication, and determination of the cell doubling-time distribution function from the decay of synchronization. The text then examines nuclear events in synchronously dividing yeast cultures, periodic enzyme synthesis in synchronous cultures of yeast, and light-induced synchronous sporulation of a myxomycete. Topics include periodicity of enzyme synthesis, use of synchronous cultures for studying cellular biosynthesis, and cytological observations on synchronously dividing cultures. The book explores the effects of temperature on the mitotic cycle of normal and synchronized mammalian cells; behavior of sulfhydryl groups in synchronous division; and morphogenetic and macromolecular aspects of synchronized Tetrahymena. The selection is highly recommended for researchers interested in biosynthetic regulation.
Author: Tim Humphrey Publisher: Humana ISBN: 9781588291448 Category : Science Languages : en Pages : 404
Book Description
The fundamental question of how cells grow and divide has perplexed biologists since the development of the cell theory in the mid-19th century, when it was recognized by Virchow and others that “all cells come from cells.” In recent years, considerable effort has been applied to the identification of the basic molecules and mechanisms that regulate the cell cycle in a number of different organisms. Such studies have led to the elucidation of the central paradigms that underpin eukaryotic cell cycle control, for which Lee Hartwell, Tim Hunt, and Paul Nurse were jointly awarded the Nobel Prize for Medicine and Physiology in 2001 in recognition of their seminal contributions to this field. The importance of understanding the fundamental mechanisms that modulate cell division has been reiterated by relatively recent discoveries of links between cell cycle control and DNA repair, growth, cellular metabolism, development, and cell death. This new phase of integrated cell cycle research provides further challenges and opportunities to the biological and medical worlds in applying these basic concepts to understanding the etiology of cancer and other proliferative diseases.
Author: R. J. C. Harris Publisher: Academic Press ISBN: 1483282007 Category : Science Languages : en Pages : 352
Book Description
Cell Growth and Cell Division is a collection of papers dealing with the biochemical and cytological aspects of cell development and changes in bacterial, plant, and animal systems. One paper discusses studies on the nuclear and cytoplasmic growth of ten different strains of the genus Blepharisma, in which different types of nutrition at high and low temperatures alter the species to the extent that they became morphologically indistinguishable. The paper describes the onset of death at high and low temperatures as being preceded by a decrease in the size of the cytoplasm and a corresponding decrease in the size of the macronucleus. The moribund organisms, still possessing structure, are motionless with no distinguishable macronuclear materials. Another paper presents the response of meiotic and mitotic cells to azaguanine, chloramphenicol, ethionine, and 5-methyltryptophan. The paper describes the failure of spindle action, arrest of second division, inhibition of cytokinesis, aberrant wall synthesis, and alterations in chromosome morphology in meiosis cells. In the case of mitosis, a single enzyme—thymidine phosphorylase—shows that reagents which inhibit protein synthesis also inhibit the appearance of that enzyme if the reagent is applied one day before it normally appears. Other papers discuss control mechanisms for chromosome reproduction in the cell cycle, as well as the force of cleavage of the dividing sea urchin egg. The collection can prove valuable for bio-chemists, cellular biologists, micro-biologists, and developmental biologists.
Author: Ralf Pörtner Publisher: Humana ISBN: 9781493963188 Category : Science Languages : en Pages : 0
Book Description
Animal Cell Biotechnology: Methods and Protocols, Third Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially high-throughput synchronization, insect cell lines, transient gene and protein expression, DNA Profiling and Characterisation. Part II addresses tools for process and medium optimization as well as microcarrier technology while Part III covers monitoring of cell growth, viability and apoptosis, metabolic flux estimation, quenching methods as well as NMR-based techniques. Part IV details cultivation techniques, and Part V describes special applications, including vaccine production, baculovirus protein expression, chromatographic techniques for downstream as well as membrane techniques for virus separation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Animal Cell Biotechnology: Methods and Protocols, Third Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.
Author: Toshiyuki Nagata Publisher: Springer Science & Business Media ISBN: 3662105721 Category : Technology & Engineering Languages : en Pages : 371
Book Description
The first compilation of a wealth of knowledge on tobacco BY-2 cells, often cited as the HeLa cell line of higher plants. Basic issues of cell cycle progression, cytokinesis, cell organization and factors that are involved in these processes are covered in detail. Since the tobacco cell line is used as a tool for research in molecular and cellular biology, several chapters on such studies are also included. Further, changes of primary and secondary metabolites during culture and factors that affect these processes are treated. Last but not least, the so far unpublished historical background of the BY-2 cell line is described. This volume is a must for any scientist working in the field of plant biology.
Author: Eishi Noguchi Publisher: Humana ISBN: 9781493947287 Category : Science Languages : en Pages : 0
Book Description
A collection of new reviews and protocols from leading experts in cell cycle regulation, Cell Cycle Control: Mechanisms and Protocols, Second Edition presents a comprehensive guide to recent technical and theoretical advancements in the field. Beginning with the overviews of various cell cycle regulations, this title presents the most current protocols and state-of-the-art techniques used to generate latest findings in cell cycle regulation, such as protocols to analyze cell cycle events and molecules. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Cell Cycle Control: Mechanisms and Protocols, Second Edition will be a valuable resource for a wide audience, ranging from the experienced cell cycle researchers looking for new approaches to the junior graduate students giving their first steps in cell cycle research.
Author: Zhixiang Wang
Author: Zhixiang Wang
Author: Amanda S. Coutts
Author: A.A. Polezhaev
Author: Ivan L. Cameron
Author: Tim Humphrey
Author: R. J. C. Harris
Author: Ralf Pörtner
Author: Toshiyuki Nagata
Author: Randal Arthur Halfmann
Author: Eishi Noguchi