Author: Ashok Kumar Rathoure Publisher: ISBN: 9789351309543 Category : Medical Languages : en Pages : 328
Book Description
This book has been divided into four segments. First segment has introduction about biotechnology and its emerging fields. Second segment has 13 basic experiments for the students who are entering into the laboratory for the first time. In this segment, students will learn about the preparation of buffer and solutions, quantitative and qualitative tests about amino acids, protein, nucleic acids, (RNA & DNA) by different methods and isolation of casein protein from the milk. In the segment 2, we have elaborated about physiology. Here, the students will learn about expression of solutions in concentrations, determination of urine urea nitrogen, calculating acid number of fats, calculation of saponification number and average molecular weight of triacylglycerols, salting in, salting out and dialysis of proteins, determination of serum albumin, total cholesterol, etc., blood group analysis, ouchterlony double diffusion test and determination of haemoglobin content by haemoglobinometer. The last segment has 28 experiments about biotechnology and biological techniques for microbiology, plant tissue culture, genetic engineering and industrial biotechnology. This book will extensively assist students, teachers and academicians to further extend their knowledge beyond the course work and related subject matter but onto the practical application to gain insights of what happening new in the present era of applied science.
Author: S. Harisha Publisher: Laxmi Publications, Ltd. ISBN: 9788131803790 Category : Science Languages : en Pages : 726
Book Description
Biotechnology Is One Of The Major New Technologies Of The Twenty-First Century That Covers Multi-Disciplinary Issues, Including Recombinant DNA Techniques, Cloning, Genetics, And The Application Of Microbiology To The Production Of Goods. It Continues To Revolutionize Treatments Of Many Diseases, And It Is Used To Deal With Environmental Solutions. The Biotechnology Procedures And Experiments Handbook Provides Practicing Professionals And Biotechnology Students Over 150 Applied, Up-To-Date Laboratory Techniques And Experiments Related To Modern Topics Such As Recombinant DNA, Electrophoresis, Stem Cell Research, Genetic Engineering, Microbiology, Tissue Culture, And More. Each Lab Technique Includes 1)A Principle, 2)The Necessary Reagents, 3)A Step By Step Procedure, And 4)A Final Result. Also Included Is A Section That Shows How To Avoid Potential Pitfalls Of A Specific Experiment. The Book Is Accompanied By A CD-ROM Containing Simulations, White Papers, And Other Relevant Material To Biotechnology.
Author: Perry D. Haaland Publisher: CRC Press ISBN: 1000103706 Category : Mathematics Languages : en Pages : 284
Book Description
This book provides the first time user of statistics with an understanding of how and why statistical experimental design and analysis can be an effective problem solving tool. It presents experimental designs which are useful for small screening and response surface experiments.
Author: M. P. Bansal Publisher: The Energy and Resources Institute (TERI) ISBN: 8179933792 Category : Science Languages : en Pages : 392
Book Description
Molecular Biology and Biotechnology: basic experimental protocols is a compilation of methods and techniques commonly used in biomedical and biotechnological studies. The book aims to provide ample support to both students and faculty while conducting practical lessons. Four sections are covered in this book—Genomics, Proteomics, Quantitative Biochemistry, and Bioinformatics. A concise introductory note accompanies each protocol/method described for better comprehension. Every topic discussed is supported by actual methods and their expected results, and is accompanied by relevant questions.
Author: Frank H. Stephenson Publisher: Academic Press ISBN: 012375691X Category : Science Languages : en Pages : 520
Book Description
Calculations for Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory, Second Edition, provides an introduction to the myriad of laboratory calculations used in molecular biology and biotechnology. The book begins by discussing the use of scientific notation and metric prefixes, which require the use of exponents and an understanding of significant digits. It explains the mathematics involved in making solutions; the characteristics of cell growth; the multiplicity of infection; and the quantification of nucleic acids. It includes chapters that deal with the mathematics involved in the use of radioisotopes in nucleic acid research; the synthesis of oligonucleotides; the polymerase chain reaction (PCR) method; and the development of recombinant DNA technology. Protein quantification and the assessment of protein activity are also discussed, along with the centrifugation method and applications of PCR in forensics and paternity testing. - Topics range from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology - Each chapter includes a brief explanation of the concept and covers necessary definitions, theory and rationale for each type of calculation - Recent applications of the procedures and computations in clinical, academic, industrial and basic research laboratories are cited throughout the text New to this Edition: - Updated and increased coverage of real time PCR and the mathematics used to measure gene expression - More sample problems in every chapter for readers to practice concepts
Author: Michael Fry Publisher: Academic Press ISBN: 012802108X Category : Science Languages : en Pages : 572
Book Description
Landmark Experiments in Molecular Biology critically considers breakthrough experiments that have constituted major turning points in the birth and evolution of molecular biology. These experiments laid the foundations to molecular biology by uncovering the major players in the machinery of inheritance and biological information handling such as DNA, RNA, ribosomes, and proteins. Landmark Experiments in Molecular Biology combines an historical survey of the development of ideas, theories, and profiles of leading scientists with detailed scientific and technical analysis. - Includes detailed analysis of classically designed and executed experiments - Incorporates technical and scientific analysis along with historical background for a robust understanding of molecular biology discoveries - Provides critical analysis of the history of molecular biology to inform the future of scientific discovery - Examines the machinery of inheritance and biological information handling
Author: Kenneth G. Rainis Publisher: Franklin Watts ISBN: 9780531159132 Category : Juvenile Nonfiction Languages : en Pages : 160
Book Description
Gives instructions for and explains the principles behind a variety of biotechnology experiments from the simple to the more difficult.
Author: David J. Glass Publisher: CSHL Press ISBN: 0879697350 Category : Biology Languages : en Pages : 211
Book Description
The effective design of scientific experiments is critical to success, yet graduate students receive very little formal training in how to do it. Based on a well-received course taught by the author, Experimental Design for Biologistsfills this gap. Experimental Design for Biologistsexplains how to establish the framework for an experimental project, how to set up a system, design experiments within that system, and how to determine and use the correct set of controls. Separate chapters are devoted to negative controls, positive controls, and other categories of controls that are perhaps less recognized, such as “assumption controls†and “experimentalist controls†. Furthermore, there are sections on establishing the experimental system, which include performing critical “system controls†. Should all experimental plans be hypothesis-driven? Is a question/answer approach more appropriate? What was the hypothesis behind the Human Genome Project? What color is the sky? How does one get to Carnegie Hall? The answers to these kinds of questions can be found in Experimental Design for Biologists. Written in an engaging manner, the book provides compelling lessons in framing an experimental question, establishing a validated system to answer the question, and deriving verifiable models from experimental data. Experimental Design for Biologistsis an essential source of theory and practical guidance in designing a research plan.
Author: P.R. Yadav Publisher: Discovery Publishing House ISBN: 9788183560207 Category : Biotechnology Languages : en Pages : 324
Book Description
Contents: Introduction, Separation Techniques, Tools for Biotechnology, Auto Analyzer, Additional Techniques, Techniques for Bioreactor, Statistical Analysis, Statistical
Author: Surajit Das Publisher: Springer ISBN: 8132220951 Category : Science Languages : en Pages : 252
Book Description
Microorganisms play an important role in the maintenance of the ecosystem structure and function. Bacteria constitute the major part of the microorganisms and possess tremendous potential in many important applications from environmental clean up to the drug discovery. Much advancement has been taken place in the field of research on bacterial systems. This book summarizes the experimental setups required for applied microbiological studies. Important background information, representative results, step by step protocol in this book will be of great use to the students, early career researchers as well as the academicians. The book describes many experiments covering the basic microbiological experiments to the applications of microbial systems for advanced research. Researchers in any field who utilize bacterial systems will find this book very useful. In addition to microbiology and bacteriology, this book will also find useful in molecular biology, genetics, and pathology and the volume should prove to be a valuable laboratory resource in clinical and environmental microbiology, microbial genetics and agricultural research. Unique features • Easy to follow by the users as the experiments have been written in simple language and step-wise manner. • Role of each reagents to be used in each experiment have been described which will help the beginners to understand quickly and design their own experiment. • Each experiment has been equipped with the coloured illustrations for proper understanding of the concept. • Trouble-shootings at the end of each experiment will be helpful in overcoming the problems faced by the users. • Flow-chart of each experiment will quickly guide the users in performing the experiments.
Author: Ashok Kumar Rathoure
Author: S. Harisha
Author: Perry D. Haaland
Author: M. P. Bansal
Author: Frank H. Stephenson
Author: Michael Fry
Author: Kenneth G. Rainis
Author: David J. Glass
Author: P.R. Yadav
Author: Surajit Das