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Author: P.L. Dubin Publisher: Elsevier ISBN: 0080858449 Category : Science Languages : en Pages : 473
Book Description
The rapid development of new packings for aqueous size-exclusion chromatography has revolutionized this field. High resolution non-adsorptive columns now make possible the efficient separation of proteins and the rapid and precise determination of the molecular weight distribution of synthetic polymers. This technology is also being applied to the separation of small ions, the characterization of associating systems, and the measurement of branching. At the same time, fundamental studies are elucidating the mechanisms of the various chromatographicprocesses.These developments in principles and applications are assembled for the first time in this book. Fundamental issues are dealt with: the roles of pore structure and macromolecular dimensions, hydrophobic and electrostatic effects, and the determination and control of column efficiency. High-performance packings based on derivatized silica are reviewed in detail. Special techniques are thoroughly described, including SEC/LALLS, inverse exclusion chromatography, and frontal zone chromatography. Attention is focussed on special applications of size-exclusion methods, such as the characterization of micelles, separations of inorganic ions, and Hummel-Dreyer and related methods for equilibrium systems. Protein chromatography is dealt with in both dedicated sections and throughout the book as a whole.This is a particularly comprehensive and authoritative work - all the contributions review broad topics of general significance and the authors are of high repute. The material will be of special value for the characterization of synthetic water-soluble polymers, especially polyelectrolytes. Biochemists will find fundamental and practical guidance on protein separations. Researchers confronted with solutes that exhibit complex chromatographic behavior, such as humic acids, aggregating proteins, and micelles should find the contents of this volume illuminating.
Author: P.L. Dubin Publisher: Elsevier ISBN: 0080858449 Category : Science Languages : en Pages : 473
Book Description
The rapid development of new packings for aqueous size-exclusion chromatography has revolutionized this field. High resolution non-adsorptive columns now make possible the efficient separation of proteins and the rapid and precise determination of the molecular weight distribution of synthetic polymers. This technology is also being applied to the separation of small ions, the characterization of associating systems, and the measurement of branching. At the same time, fundamental studies are elucidating the mechanisms of the various chromatographicprocesses.These developments in principles and applications are assembled for the first time in this book. Fundamental issues are dealt with: the roles of pore structure and macromolecular dimensions, hydrophobic and electrostatic effects, and the determination and control of column efficiency. High-performance packings based on derivatized silica are reviewed in detail. Special techniques are thoroughly described, including SEC/LALLS, inverse exclusion chromatography, and frontal zone chromatography. Attention is focussed on special applications of size-exclusion methods, such as the characterization of micelles, separations of inorganic ions, and Hummel-Dreyer and related methods for equilibrium systems. Protein chromatography is dealt with in both dedicated sections and throughout the book as a whole.This is a particularly comprehensive and authoritative work - all the contributions review broad topics of general significance and the authors are of high repute. The material will be of special value for the characterization of synthetic water-soluble polymers, especially polyelectrolytes. Biochemists will find fundamental and practical guidance on protein separations. Researchers confronted with solutes that exhibit complex chromatographic behavior, such as humic acids, aggregating proteins, and micelles should find the contents of this volume illuminating.
Author: Sadao Mori Publisher: Springer Science & Business Media ISBN: 3662039109 Category : Technology & Engineering Languages : en Pages : 236
Book Description
Although size exclusion chromatography (SEC) is perhaps the most popular and widely used technique for determining the molecular weight distribution of polymeric materials, there have been very few texts written on this topic. During the past decade, SEC has experienced a considerable amount of growth in regard to column and detector technology and new applications. With these advances, SEC can now be used for determining absolute molecular weight, polymer chain conformation and size, and branching, as well as polymer solution properties. This book introduces the reader to the fundamentals of SEC with emphasis on practical aspects of the technique, such as column and mobile selection, calibration, new detector capabilities and guidelines for performing SEC on most types of polymers, especially those of industrial importance. This book is intended for either those new to the field of SEC, or for those research workers who require a more comprehensive background.
Author: Andre Striegel Publisher: John Wiley & Sons ISBN: 0470442832 Category : Science Languages : en Pages : 512
Book Description
The Second Edition of Modern Size-Exclusion Chromatography offers a complete guide to the theories, methods, and applications of size-exclusion chromatography. It provides an unparalleled, integrated, up-to-date treatment of gel permeation and gel filtration chromatography. With its detailed descriptions of techniques, data handling, compilations of information on columns and column packings, and tables of important solvents and reference materials, the book offers readers everything they need to take full advantage of this popular macromolecular characterization technique. Since publication of the first edition in 1979, there have been many important advances in the field of size-exclusion chromatography. This Second Edition brings the book thoroughly up to date, with expert coverage of: New and emerging industrial and research applications Practical aspects of size-exclusion chromatography (SEC) and multidetector and multidimensional SEC technologies for polymer architecture and copolymer analysis Updated information on the latest equipment and techniques New best practices for the lab SEC in relation to polymer characterization techniques such as GPEC, LCCC, and rheology Throughout the text, detailed examples guide you step by step through all the latest techniques and applications. With its extensive revisions and updates written by leading experts and pioneers in the field, Modern Size-Exclusion Liquid Chromatography remains the definitive resource for the broad range of researchers and scientists who use HPLC and GPC methods.
Author: Chi-san Wu Publisher: Elsevier ISBN: 0080529399 Category : Science Languages : en Pages : 663
Book Description
Column Handbook for Size Exclusion Chromatography is the first comprehensive reference to provide everything one needs to know about commercial analytical and preparative columns for size exclusion and gel filtration chromatography (SEC and GFC). SEC is now widely used as a quality assurance method in the polymer industry (both synthetic and biopolymers) to determine molecular weight and molecular weight distribution. The Handbook contains contributions from every column manufacturer around the world and from many experienced column users. It covers the technology, characterization, application, evaluation, maintenance, and quality control of analytical and preparative columns for SEC and GFC. Also included are columns for two closely related techniques, hydrodynamic chromatography and high osmotic pressure chromatography.Key Features* Evaluate and select columns with confidence for specific applications* Optimize separations and improve the ruggedness of analytical methods* Extend the service time of a column* Establish a quality-control program to ensure consistency in column performance* Avoid the expense of column damage or purchases that do not give the expected results
Author: B. J. Hunt Publisher: Springer Science & Business Media ISBN: 1461578612 Category : Science Languages : en Pages : 292
Book Description
There is a large and increasing variety of polymers currently in use both for domestic and industrial applications. The properties of polymers are deter mined not only by their chemical type, but also by their molecular mass and molecular mass distributions. However, while the chemical type of polymers can be determined relatively easily, the average molecular masses and molecular mass distributions are more difficult to measure. The molecular mass averages of a polymer are measured by specialized and complex techniques such as light scattering (for weight average) and osmometry (for number average). Thus, complete characterization of the molecular mass distribution of a polymer by such means requires separating the sample into many fractions which can then be examined individually. Since size exclusion chromatography was introduced as a rapid and straightforward technique for the characterization of polymer molecular mass distributions, there have been tremendous increases in development and applications, and it was felt appropriate to bring together into a single volume the information required by scientists from many disciplines who wish to use the technique. This book should be useful to existing users, those who are new to the technique, and those who may be familiar with the basic technique and now wish to extend their capabilities to more complex applications (or to consider the potential of a number of related techniques). The book will also be of general interest to the experienced liquid chromatographer.
Author: Chi-San Wu Publisher: CRC Press ISBN: 0824757793 Category : Science Languages : en Pages : 721
Book Description
Documenting critical advances in this rapidly evolving field, the Second Edition highlights the need for new applications and technologies that assist in the determination of molecular weight and molecular weight distributions of polymers in an accurate, efficient manner. This volume presents the latest findings from a international team of specialists and continues to inspire and extend practical applications of size exclusion chromatography (SEC). It includes six new chapters covering high-speed size exclusion chromatography, SEC of low molecular weight materials, and the extended family of techniques, from two-dimensional liquid chromatography to high osmotic pressure chromatography.
Author: Sabu Thomas Publisher: Elsevier ISBN: 032346145X Category : Technology & Engineering Languages : en Pages : 294
Book Description
Thermal and Rheological Measurement Techniques for Nanomaterials Characterization, Second Edition covers thermal and rheological measurement techniques, including their principle working methods, sample preparation and interpretation of results. This important reference is an ideal source for materials scientists and industrial engineers who are working with nanomaterials and need to know how to determine their properties and behaviors. - Outlines key characterization techniques to determine the thermal and rheological behavior of different nanomaterials - Explains how the thermal and rheological behavior of nanomaterials affect their usage - Provides a method-orientated approach that explains how to successfully use each technique
Author: Nelu Grinberg Publisher: CRC Press ISBN: 1482218682 Category : Medical Languages : en Pages : 975
Book Description
This handbook is a guide for workers in analytical chemistry who need a starting place for information about a specific instrumental technique. It gives a basic introduction to the techniques and provides leading references on the theory and methodology for an instrumental technique. This edition thoroughly expands and updates the chapters to include concepts, applications, and key references from recent literature. It also contains a new chapter on process analytical technology.
Author: Muhammad Imran Malik Publisher: Elsevier ISBN: 012822598X Category : Technology & Engineering Languages : en Pages : 670
Book Description
Molecular Characterization of Polymers presents a range of advanced and cutting-edge methods for the characterization of polymers at the molecular level, guiding the reader through theory, fundamentals, instrumentation, and applications, and supporting the end goal of efficient material selection and improved material performance. Each chapter focuses on a specific technique or family of techniques, including the different areas of chromatography, field flow fractionation, long chain branching, static and dynamic light scattering, mass spectrometry, NMR, X-Ray and neutron scattering, polymer dilute solution viscometry, microscopy, and vibrational spectroscopy. In each case, in-depth coverage explains how to successfully implement and utilize the technique. This practical resource is highly valuable to researchers and advanced students in polymer science, materials science, and engineering, and to those from other disciplines and industries who are unfamiliar with polymer characterization techniques. - Introduces a range of advanced characterization methods, covering aspects such as molecular weight, polydispersity, branching, composition, and tacticity - Enables the reader to understand and to compare the available technique, and implement the selected technique(s), with a view to improving properties of the polymeric material - Establishes a strong link between basic principles, characterization techniques, and real-life applications
Author: P.L. Dubin
Author: P.L. Dubin
Author: Sadao Mori
Author: Andre Striegel
Author: Chi-san Wu
Author: B. J. Hunt
Author: Chi-San Wu
Author: Paul Dubin
Author: Sabu Thomas
Author: Nelu Grinberg
Author: Muhammad Imran Malik