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Application of Genomic Assisted Breeding for Improvement of Barley Cultivars

Application of Genomic Assisted Breeding for Improvement of Barley Cultivars PDF Author: William Duke Pauli
Publisher:
ISBN:
Category : Barley
Languages : en
Pages : 292

Book Description
The use of genome-wide association studies (GWAS) to detect quantitative trait loci (QTL) controlling complex traits has become a popular approach for studying key traits in crop plants. The goal of this research was to identify regions of the barley (Hordeum vulgare L.) genome that impact both agronomic and malting quality traits. By identifying these regions of the genome and their associated diagnostic markers, we gain an understanding of the genetic architecture of the traits as well as develop informative markers that can be utilized for marker-assisted selection. We used the data generated by the Barley Coordinated Agricultural Program to identify marker-trait associations impacting agronomic performance using a Q+K mixed linear model accounting for population structure and relatedness among lines. This data was also used to develop a genotyping platform specific to the Montana State University (MSU) Barley Breeding Program. This genotyping platform was used to genotype 650 advance generation lines from eleven bi-parental families to investigate the genetic basis of malting quality traits and the regions of the barley genome impacting them. We detected 41 significant marker-trait associations for the agronomic traits we studied with 31 of those being previously detected in bi-parental mapping studies. We detected 54 significant marker-trait associations for the malting quality traits with 24 of those being previously reported. The combined results from both studies indicate that major genes impacting key traits in barley are still segregating in US germplasm as well as in the MSU germplasm. This demonstrates that there is useful standing genetic variation that can be utilized for superior barley cultivar development and further genetic gain. Furthermore, by identifying the beneficial alleles, and their associated markers, we can form a "catalog" of major genes and QTL impacting agronomic and malting quality traits which can be used for marker-assisted selection. This work also demonstrates the feasibility and utility of conducting GWAS in narrow germplasm arrays like those found in regional breeding programs and serves as a paradigm for other cereal breeding programs. Together, these studies show how genomic data can be leveraged for varietal improvement in regional plant breeding programs.

Application of Genomic Assisted Breeding for Improvement of Barley Cultivars

Application of Genomic Assisted Breeding for Improvement of Barley Cultivars PDF Author: William Duke Pauli
Publisher:
ISBN:
Category : Barley
Languages : en
Pages : 292

Book Description
The use of genome-wide association studies (GWAS) to detect quantitative trait loci (QTL) controlling complex traits has become a popular approach for studying key traits in crop plants. The goal of this research was to identify regions of the barley (Hordeum vulgare L.) genome that impact both agronomic and malting quality traits. By identifying these regions of the genome and their associated diagnostic markers, we gain an understanding of the genetic architecture of the traits as well as develop informative markers that can be utilized for marker-assisted selection. We used the data generated by the Barley Coordinated Agricultural Program to identify marker-trait associations impacting agronomic performance using a Q+K mixed linear model accounting for population structure and relatedness among lines. This data was also used to develop a genotyping platform specific to the Montana State University (MSU) Barley Breeding Program. This genotyping platform was used to genotype 650 advance generation lines from eleven bi-parental families to investigate the genetic basis of malting quality traits and the regions of the barley genome impacting them. We detected 41 significant marker-trait associations for the agronomic traits we studied with 31 of those being previously detected in bi-parental mapping studies. We detected 54 significant marker-trait associations for the malting quality traits with 24 of those being previously reported. The combined results from both studies indicate that major genes impacting key traits in barley are still segregating in US germplasm as well as in the MSU germplasm. This demonstrates that there is useful standing genetic variation that can be utilized for superior barley cultivar development and further genetic gain. Furthermore, by identifying the beneficial alleles, and their associated markers, we can form a "catalog" of major genes and QTL impacting agronomic and malting quality traits which can be used for marker-assisted selection. This work also demonstrates the feasibility and utility of conducting GWAS in narrow germplasm arrays like those found in regional breeding programs and serves as a paradigm for other cereal breeding programs. Together, these studies show how genomic data can be leveraged for varietal improvement in regional plant breeding programs.

The Barley Genome

The Barley Genome PDF Author: Nils Stein
Publisher: Springer
ISBN: 3319925288
Category : Science
Languages : en
Pages : 400

Book Description
This book presents an overview of the state-of-the-art in barley genome analysis, covering all aspects of sequencing the genome and translating this important information into new knowledge in basic and applied crop plant biology and new tools for research and crop improvement. Unlimited access to a high-quality reference sequence is removing one of the major constraints in basic and applied research. This book summarizes the advanced knowledge of the composition of the barley genome, its genes and the much larger non-coding part of the genome, and how this information facilitates studying the specific characteristics of barley. One of the oldest domesticated crops, barley is the small grain cereal species that is best adapted to the highest altitudes and latitudes, and it exhibits the greatest tolerance to most abiotic stresses. With comprehensive access to the genome sequence, barley’s importance as a genetic model in comparative studies on crop species like wheat, rye, oats and even rice is likely to increase.

Genomics-assisted Breeding of Malting Barley for New York State

Genomics-assisted Breeding of Malting Barley for New York State PDF Author: Daniel William Sweeney
Publisher:
ISBN:
Category :
Languages : en
Pages : 0

Book Description
Barley (Hordeum vulgare L.) has been the primary ingredient in brewing for millennia. Recent increases in demand for local malting barley production outside of western North America for craft brewing and distilling have created a need for new varieties adapted to humid continental climates. Malting quality can be negatively affected by several seed pathogens and preharvest sprouting (PHS) in regions with wet conditions during the growing season. Genomics-assisted breeding methods, including genomic prediction and genome-wide association, have the potential to improve selection accuracy and reduce breeding cycle time to increase rates of genetic gain. My goals were to apply genomics-assisted breeding methods to address malting barley production challenges in the northeastern United States. Specific objectives included 1) examining the genetic architecture and environmental stability of the seed dormancy 2 (SD2) locus to determine if tight linkage or pleiotropy is responsible for negative correlations between PHS and malting quality, 2) studying the genetic relationships between PHS, seed dormancy, and seed germination rate to identify germplasm with PHS resistance and good malting quality, 3) exploring strategies to enhance genomic prediction accuracy in a newly established spring barley breeding program, 4) evaluating the merits of a genomic selection approach using a selection index in a newly established breeding program, and 5) releasing a two-row spring malting barley variety with disease resistance, PHS resistance, and good malting quality for local growers. The key findings of this research are 1) three functional alleles of HvMKK3 are the primary determinants of PHS resistance and seed dormancy at SD2 and HvGA20ox1 does not have an effect on these traits, 2) HvMKK3 and HvAlaAT1 are sensitive to environmental conditions and together explain a substantial portion of annual PHS variation, 3) a connected half-sib training population increases within and across family prediction accuracy and is highly powered for genome-wide association studies, 4) genomic selection using a selection index resulted in significant gain for highly weighted traits but retained genetic variance for lightly weighted traits, and 5) a new two-row spring barley variety, 'Excelsior Gold', was released as a result of these combined efforts.

Genomics Assisted Breeding of Crops for Abiotic Stress Tolerance, Vol. II

Genomics Assisted Breeding of Crops for Abiotic Stress Tolerance, Vol. II PDF Author: Vijay Rani Rajpal
Publisher: Springer
ISBN: 3319995731
Category : Science
Languages : en
Pages : 260

Book Description
The abiotic stresses like drought, temperature, cold, salinity, heavy metals etc. affect a great deal on the yield performance of the agricultural crops. To cope up with these challenges, plant breeding programs world-wide are focussing on the development of stress tolerant varieties in all crop species. Significant genomic advances have been made for abiotic stress tolerance in various crop species in terms of availability of molecular markers, QTL mapping, genome-wide association studies (GWAS), genomic selection (GS) strategies, and transcriptome profiling. The broad-range of articles involving genomics and breeding approaches deepens our existing knowledge about complex traits. The chapters are written by authorities in their respective fields. This book provides comprehensive and consolidated account on the applications of the most recent findings and the progress made in genomics assisted breeding for tolerance to abiotic stresses in many important major crop species with a focus on applications of modern strategies for sustainable agriculture. The book is especially intended for students, molecular breeders and scientists working on the genomics-assisted genetic improvement of crop species for abiotic stress tolerance.

Genetic and Genomic Resources for Grain Cereals Improvement

Genetic and Genomic Resources for Grain Cereals Improvement PDF Author: Mohar Singh
Publisher: Academic Press
ISBN: 0128020377
Category : Business & Economics
Languages : en
Pages : 386

Book Description
Genetic and Genomic Resources For Cereals Improvement is the first book to bring together the latest available genetic resources and genomics to facilitate the identification of specific germplasm, trait mapping, and allele mining that are needed to more effectively develop biotic and abiotic-stress-resistant grains. As grain cereals, including rice, wheat, maize, barley, sorghum, and millets constitute the bulk of global diets, both of vegetarian and non-vegetarian, there is a greater need for further genetic improvement, breeding, and plant genetic resources to secure the future food supply. This book is an invaluable resource for researchers, crop biologists, and students working with crop development and the changes in environmental climate that have had significant impact on crop production. It includes the latest information on tactics that ensure that environmentally robust genes and crops resilient to climate change are identified and preserved. Provides a single-volume resource on the global research work on grain cereals genetics and genomics Presents information for effectively managing and utilizing the genetic resources of this core food supply source Includes coverage of rice, wheat, maize, barley, sorghum, and pearl, finger and foxtail millets

Plant Breeding: Past, Present and Future

Plant Breeding: Past, Present and Future PDF Author: John E. Bradshaw
Publisher: Springer
ISBN: 3319232851
Category : Science
Languages : en
Pages : 710

Book Description
This book aims to help plant breeders by reviewing past achievements, currently successful practices, and emerging methods and techniques. Theoretical considerations are also presented to strike the right balance between being as simple as possible but as complex as necessary. The United Nations predicts that the global human population will continue rising to 9.0 billion by 2050. World food production will need to increase between 70-100 per cent in just 40 years. First generation bio-fuels are also using crops and cropland to produce energy rather than food. In addition, land area used for agriculture may remain static or even decrease as a result of degradation and climate change, despite more land being theoretically available, unless crops can be bred which tolerate associated abiotic stresses. Lastly, it is unlikely that steps can be taken to mitigate all of the climate change predicted to occur by 2050, and beyond, and hence adaptation of farming systems and crop production will be required to reduce predicted negative effects on yields that will occur without crop adaptation. Substantial progress will therefore be required in bridging the yield gap between what is currently achieved per unit of land and what should be possible in future, with the best farming methods and best storage and transportation of food, given the availability of suitably adapted cultivars, including adaptation to climate change. My book is divided into four parts: Part I is an historical introduction; Part II deals with the origin of genetic variation by mutation and recombination of DNA; Part III explains how the mating system of a crop species determines the genetic structure of its landraces; Part IV considers the three complementary options for future progress: use of sexual reproduction in further conventional breeding, base broadening and introgression; mutation breeding; and genetically modified crops.

Genetics and Improvement of Barley Malt Quality

Genetics and Improvement of Barley Malt Quality PDF Author: Guoping Zhang
Publisher: Springer Science & Business Media
ISBN: 3642012795
Category : Science
Languages : en
Pages : 309

Book Description
Genetics and Improvement of Barley Malt Quality presents up-to-date developments in barley production and breeding. The book is divided into nine chapters, including barley production and consumption, germplasm and utilization, chemical composition, protein and protein components, carbohydrates and sugars, starch degrading enzymes, endosperm cell walls and malting quality, genomics and malting quality improvement, and marker-assisted selection for malting quality. The information will be especially useful to barley breeders, malsters, brewers, biochemists, barley quality specialists, molecular geneticists, and biotechnologists. This book may also serve as reference text for post-graduate students and barley researchers. The authors for each chapter are the experts and frontier researchers in the specific areas. Professor Guoping Zhang is a barley breeder and crop physiologist in Department of Agronomy, Zhejiang University of China. Dr. Chengdao Li is a senior molecular geneticist and barley breeder in Department of Agriculture & Food, Western Australia. He is also an adjunct professor in Murdoch University of Australia and Zhejiang University of China.

Translational Genomics for Crop Breeding, Volume 2

Translational Genomics for Crop Breeding, Volume 2 PDF Author: Rajeev Varshney
Publisher: John Wiley & Sons
ISBN: 0470962917
Category : Science
Languages : en
Pages : 274

Book Description
Genomic Applications for Crop Breeding: Abiotic Stress, Quality and Yield Improvement is the second of two volumes looking at the latest advances in genomic applications to crop breeding. This volume focuses on advances improving crop resistance to abiotic stresses such as extreme heat, drought, flooding as well as advances made in quality and yield improvement. Chapters examine advances in such key crops as rice, maize, and sugarcane, among others. Genomic Applications for Crop Breeding: Abiotic Stress, Quality and Yield Improvement complements the earlier volume on biotic stressors and will be an essential purchase for those interested in crop science and food production.

Translational Genomics for Crop Breeding, Volume 2

Translational Genomics for Crop Breeding, Volume 2 PDF Author: Rajeev Varshney
Publisher: John Wiley & Sons
ISBN: 1118728378
Category : Science
Languages : en
Pages : 274

Book Description
Genomic Applications for Crop Breeding: Abiotic Stress, Quality and Yield Improvement is the second of two volumes looking at the latest advances in genomic applications to crop breeding. This volume focuses on advances improving crop resistance to abiotic stresses such as extreme heat, drought, flooding as well as advances made in quality and yield improvement. Chapters examine advances in such key crops as rice, maize, and sugarcane, among others. Genomic Applications for Crop Breeding: Abiotic Stress, Quality and Yield Improvement complements the earlier volume on biotic stressors and will be an essential purchase for those interested in crop science and food production.

Genomics-Assisted Crop Improvement

Genomics-Assisted Crop Improvement PDF Author: Rajeev K. Varshney
Publisher: Springer Science & Business Media
ISBN: 1402062974
Category : Technology & Engineering
Languages : en
Pages : 517

Book Description
This superb volume provides a critical assessment of genomics tools and approaches for crop breeding. Volume 1 presents the status and availability of genomic resources and platforms, and also devises strategies and approaches for effectively exploiting genomics research. Volume 2 goes into detail on a number of case studies of several important crop and plant species that summarize both the achievements and limitations of genomics research for crop improvement.