Analysis of Two Aspects of Zebrafish Primordial Germ Cell Development PDF Download

Author: Uta Wolke
Publisher:
ISBN:
Category :
Languages : en
Pages : 144

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Author: Markus J. Strasser
Publisher:
ISBN:
Category :
Languages : en
Pages : 254

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Author: Celeste Eno
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages :

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Book Description
Animals specify primordial germ cells (PGCs) in two alternate modes: preformation and epigenesis. Epigenesis relies on signal transduction from the surrounding tissues to instruct a group of cells to acquire PGC identity. Preformation, thought to be the more derived PGC specification mode, is instead based on the maternal inheritance of germ cell-determining factors. We use the zebrafish as a model system, in which PGCs are specified through maternal inheritance of germ plasm, to study this process in vertebrates. In zebrafish, maternally inherited germ plasm ribonucleoparticles (RNPs) have co-opted the cytoskeletal machinery to reach progressive levels of multimerization, resulting in the formation of four large masses of aggregated germ plasm RNPs. At later stages, germ plasm masses continue to use components of the cell division machinery, such as the spindles, centrosomes, and/or subcellular organelles to segregate asymmetrically during cell division and subsequently induce germ cell fate. This chapter discusses the current knowledge of germ cell specification focusing on the zebrafish as a model system. We also provide a comparative analysis of the mechanism for germ plasm RNP segregation in zebrafish versus other known vertebrate systems of germ cell preformation, such as in amphibian and avian models.

Author: Lilianna Solnica-Krezel
Publisher: Springer
ISBN: 9783642536670
Category : Science
Languages : en
Pages : 438

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Authored by leading experts in the field, this book provides the first comprehensive overview of the mechanisms of early patterning and morphogenesis in zebrafish. It summarizes the current knowledge and the key questions for the next decade of research.

Author: Florence Louise Marlow
Publisher: Morgan & Claypool Publishers
ISBN: 161504051X
Category : Family & Relationships
Languages : en
Pages : 221

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Eggs of all animals contain mRNAs and proteins that are supplied to or deposited in the egg as it develops during oogenesis. These maternal gene products regulate all aspects of oocyte development, and an embryo fully relies on these maternal gene products for all aspects of its early development, including fertilization, transitions between meiotic and mitotic cell cycles, and activation of its own genome. Given the diverse processes required to produce a developmentally competent egg and embryo, it is not surprising that maternal gene products are not only essential for normal embryonic development but also for fertility. This review provides an overview of fundamental aspects of oocyte and early embryonic development and the interference and genetic approaches that have provided access to maternally regulated aspects of vertebrate development. Some of the pathways and molecules highlighted in this review, in particular, Bmps, Wnts, small GTPases, cytoskeletal components, and cell cycle regulators, are well known and are essential regulators of multiple aspects of animal development, including oogenesis, early embryogenesis, organogenesis, and reproductive fitness of the adult animal. Specific examples of developmental processes under maternal control and the essential proteins will be explored in each chapter, and where known conserved aspects or divergent roles for these maternal regulators of early vertebrate development will be discussed throughout this review. Table of Contents: Introduction / Oogenesis: From Germline Stem Cells to Germline Cysts / Oocyte Polarity and the Embryonic Axes: The Balbiani Body, an Ancient Oocyte Asymmetry / Preparing Developmentally Competent Eggs / Egg Activation / Blocking Polyspermy / Cleavage/ Mitosis: Going Multicellular / Maternal-Zygotic Transition / Reprogramming: Epigenetic Modifications and Zygotic Genome Activation / Dorsal-Ventral Axis Formation before Zygotic Genome Activation in Zebrafish and Frogs / Maternal TGF-β and the Dorsal-Ventral Embryonic Axis / Maternal Control After Zygotic Genome Activation / Compensation by Stable Maternal Proteins / Maternal Contributions to Germline Establishment or Maintenance / Perspective / Acknowledgments / References

Author: Francisco F. Nogales
Publisher: Springer
ISBN: 3662537753
Category : Medical
Languages : en
Pages : 453

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This book provides, for the first time, a detailed, up-to-date, holistic approach to the pathology and biology of germ cell tumors. The main focus is the complex histopathology of these tumors, but special considerations are also given to their morphologic variations in relation to age, gender and organ. Critical updates are provided on terminology and classification; the discussion of biology and pathogenesis includes references to many molecular genetic and immunohistochemical findings from recent stem cell research. Individual chapters are devoted to germ cell tumors at different anatomic locations, including not only the ovaries and testes but also the mediastinum and central nervous system, and pediatric tumors. All of the contributing authors are opinion leaders from universities of excellence in Europe and the United States. This monograph will be of wide interest to practitioners and researchers in many fields of medicine, including pathology, gynecology, urology, and pediatrics, as well as stem cell scientists.

Author: Steven X. Hou
Publisher: Humana Press
ISBN: 9781617378805
Category : Science
Languages : en
Pages : 0

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Book Description
In this comprehensive and cutting-edge book, leading experts explore the parameters that define germline stem cells and the mechanisms that regulate the cell behavior in order to better isolate, characterize and maintain them. The volume begins by providing protocols for germline stem cell identification and regulation in model organisms, and concludes with detailed chapters covering current techniques involving in vitro culture and the applications of the cells.

Author: Ignacio Fernandez
Publisher: Academic Press
ISBN: 0128222743
Category : Science
Languages : en
Pages : 438

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Book Description
Cellular and Molecular Approaches in Fish Biology is a highly interdisciplinary resource to bring industry professionals, students and researchers up-to-date with the latest developments and information on fish biology research combining a historical overview of the different research areas in fish biology and detailed descriptions of cellular and molecular approaches with explanations and recommendations for research. The book presents a global perspective of each research area with detailed analytical methodologies on the cellular and molecular mechanisms within fish biology for expermentation. The book provides different points of view on how researchers have addressed timely issues, while describing and dissecting some of the new experimental/analytical approaches used to answer the key questions at cellular and molecular levels, making this a valuable resource to those in industry and academia as well as those entering the field. Provides detailed descriptions of each research approach, highlighting the tricks of the trade for its effective and successful application Includes the latest developments in fish reproduction, fish development and nutrition, fish welfare, fish immunology, ecology and biomedics Presents hot topics of research such as genetics, transcriptomics and epigenetics

Author: Patrick J. Babin
Publisher: Springer Science & Business Media
ISBN: 1402062338
Category : Nature
Languages : en
Pages : 513

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Book Description
This book addresses the growing needs in deciphering the biological processes associated with fish reproduction, in view of the growth of aquaculture and the dwindling natural stocks of commercially important fish. It presents a comprehensive overview on egg production in fish, from the standpoint of the oocyte. With this view in mind, the book includes chapters on oocyte development (oogenesis), hormonal regulation and hormone receptors, formation of the egg envelopes, growth, accumulation of nutrients and maternal transcripts, maturation, hydration, ovulation and fertilization. A special emphasis is placed on using state-of-the-art tools including electron microscopy for discerning the ultra-structure of the follicle and genomic/proteomic tools to fully understand biological basis of fish reproduction. Studies on promoting oocyte maturation, ovulation and spawning in farmed fish and preservation of fish oocytes at low temperatures are also included. The book will appeal to University lecturers, students, research scientists and those associated with culture of fish in freshwater or marine aquaculture.

Author: Kiyoshi Naruse
Publisher: Springer Science & Business Media
ISBN: 4431926917
Category : Science
Languages : en
Pages : 389

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Book Description
Oryzias latipes, known as medaka, is a model organism from East Asia. Breeding of this small, egg-laying freshwater teleost fish has long been popular among hobbyists in Japan. Now, as biological science has entered the genome era, the medaka provides significant advantages that make it one of the most valuable vertebrate models: a large collection of spontaneous mutants collected over a century, the presence of highly polymorphic inbred lines established over decades, and a recently completed genome sequence. This book is the first comprehensive monograph to cover a variety of medaka research. It opens with a historical view of medaka, followed by a series of research topics in the four major areas where the medaka is increasingly important: genomics, genetics, and resources; organogenesis and disease models; germ cells, sex determination, and reproduction; and evolution. Readers will find state-of-the-art information on medaka genetics and genomics such as the first isolation of active transposons in vertebrates, the influence of chromatin structure on sequence variation, fine QTL analysis, and versatile mutants as human disease models.