Author: Jeffrey David Alls
Publisher: Library and Archives Canada = Bibliothèque et Archives Canada
ISBN: 9780494025468
Category :
Languages : en
Pages : 278

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Book Description
Traffic jam (tj) encodes a soma-specific Maf transcription factor required for morphogenesis of the gonads in Drosophila melanogaster. I examined the requirement of tj function throughout oogenesis by studying the expression of follicle cell type specific markers in tj mutant cell clones. I also examined the interaction between tj and JAK-Stat pathway genes using a variety of loss- and gain-of-function mutations. As well as regulating the expression of a number of adhesion molecules, TJ appears to be a general requirement in follicle cells for their proper differentiation throughout oogenesis. Depending on when cells lose tj, their subsequent differentiation is blocked or defective. This suggests that like other Mafs, TJ is a regulator of differentiation. Furthermore, my findings suggest that TJ interacts with the JAK-Stat pathway during oogenesis. I have also identified a possible interaction between TJ and the EGF receptor signaling pathway.

Author: Milica Gioia Arandjelovic
Publisher: National Library of Canada = Bibliothèque nationale du Canada
ISBN: 9780612915879
Category :
Languages : en
Pages : 278

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Book Description
The gene traffic jam (tj) encodes a soma-specific, large Maf transcription factor, required for gonad morphogenesis in Drosophila. My gain-of-function analysis of tj in adult ovaries revealed both, defects in cell specification and cell behaviour. The ectopic expression of tj during oogenesis produced follicles with ectopic polar cells, missing inter-follicular stalks and stalk cells, absent or non-migrating border cells, non-migrating centripetal cells and defective stretch cells. These tj gain-of-function phenotypes mimic the phenotypes found in Jak-Stat signalling pathway mutants. My data suggest that TJ may normally be involved in cell specification, and may interact with the Jak-Stat signalling pathway. It was also found that two of the mouse homologues of tj, MafB and c-Maf, are expressed in somatic tissues of adult mouse gonads, suggesting that the role of tj in gonad development and gametogenesis may be evolutionarily conserved.

Author: Felix Gunawan
Publisher:
ISBN:
Category :
Languages : en
Pages :

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Author: Jean-Baptiste Coutelis
Publisher:
ISBN:
Category :
Languages : en
Pages : 226

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Book Description
DROSOPHILA MELANOGASTER BODY AXES ARE DEFINED BY PRECISE LOCALIZATION AND RESTRICTION OF MOLECULAR DETERMINANTS IN THE OOCYTE. POLARIZATION OF THE OOCYTE DURING OOGENESIS IS CRITICAL FOR THIS PROCESS. THE DIRECTED TRAFFIC OF MEMBRANES AND PROTEINS IS A CRUCIAL COMPONENT OF POLARITY ESTABLISHMENT IN VARIOUS CELL TYPES AND ORGANISMS. DURING MY PHD WORK, I INVESTIGATED THE ROLE OF THE SMALL MONOMERIC GTPASE RAB6 IN ORGANIZATION OF THE EGG-CHAMBER AND ASYMMETRIC LOCALIZATION OF THE BODY AXIS DETERMINANTS DURING OOGENESIS. I FOUND THAT ABSENCE OF RAB6 FUNCTION CAUSES EGG-CHAMBER DISORGANIZATION AND LOSS OF NURSE CELL PLASMA MEMBRANES. I ALSO OBSERVED THAT EXOCYTOSIS IS AFFECTED IN RAB6 NULL EGG-CHAMBERS, PROVIDING AN EXPLANATION FOR THE OBSERVED PHENOTYPES. RAB6 IS ALSO REQUIRED FOR POLARIZATION OF THE OOCYTE MICROTUBULE CYTOSKELETON AND LOCALIZATION OF OSKAR MRNA AT THE OOCYTE POSTERIOR POLE. FURTHERMORE, I SHOWED THAT, IN VIVO, RAB6 IS FOUND IN A COMPLEX WITH BICAUDAL-D, KNOWN FOR ITS ROLE IN MRNA LOCALIZATION IN DROSOPHILA. GENETIC ANALYSIS REVEALS THAT, IN THE OOCYTE, RAB6 AND BICAUDAL-D COOPERATE IN OSKAR MRNA LOCALIZATION. AS A CONCLUSION, I PROPOSE THAT DURING DROSOPHILA OOGENESIS, RAB6-DEPENDENT MEMBRANE TRAFFICKING IS DOUBLY REQUIRED, FIRST FOR GENERAL ORGANIZATION AND GROWTH OF THE EGG- CHAMBER, AND SECOND, MORE SPECIFICALLY, FOR POLARIZATION OF THE MICROTUBULE CYTOSKELETON AND LOCALIZATION OF OSKAR MRNA. THESE FINDINGS HIGHLIGHT THE CENTRAL ROLE OF VESICULAR TRAFFICKING IN ESTABLISHMENT OF POLARITY AND DETERMINANT LOCALIZATION IN DROSOPHILA.

Author: David Moore
Publisher: Springer Science & Business Media
ISBN: 3642610994
Category : Science
Languages : en
Pages : 582

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Book Description
Life Science studies in space were initially driven by the need to explore how man could survive spaceflight conditions; the effects of being launched un der high accelerations, exposed to weightlessness and radiation for different periods of time, and returned to Earth in safety. In order to substantiate the detailed knowledge of potentially adverse effects, many model experiments were launched using organisms which ranged from bacteria, plants, inverte brates, rodents and primates through to man. Although no immediate life threatening effects were found, these experiments can be considered today as the precursors to life science research in space. Many unexplained effects on these life forms were attributed to the condition of weightlessness. Most of them were poorly recorded, poorly published, or left simply with anecdotal information. Only with the advent of Skylab, and later Spacelab, did the idea emerge, and indeed the infrastructure permit, weightlessness to be considered as an ex tended tool for research into some fundamental mechanisms or processes as sociated with the effect of gravity on organisms at all levels. The initial hy pothesis to extrapolate from hypergravity through 1 x g to near 0 x g effects could no longer be retained, since many of the experiment results were seen to contradict the models or theories in the current textbooks of biology and physiology. The past decade has been dedicated primarily to exploratory research.

Author: Patricia Y. Fechner
Publisher: Springer Nature
ISBN: 303034150X
Category : Medical
Languages : en
Pages : 280

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Book Description
Comprehensive and practical, this is a unique and multidisciplinary resource for the clinician caring for the girl or woman with Turner syndrome. Although approximately one in 2000 women are affected, many have not been diagnosed; as the advent of prenatal genetic testing becomes more prevalent, the diagnosis of Turner syndrome will be made much more frequently. There is therefore a greater need for this single source that provides the clinician with the information required to care for this multifaceted disorder. The opening chapters discuss the biology, genetics and current standard of care for females with Turner syndrome in order to provide proper background and context for the remaining chapters. The main section of the book, taking a body system approach, is comprised of chapters written by an expert in his or her subspecialty and will discuss pathophysiology and diagnosis, as well as therapeutic options. Reproductive, cardiac, renal, endocrine, neurologic, musculoskeletal, and sensory issues and symptoms associated with Turner syndrome are all covered in detail. Additional chapters describe current resources available to both caregiver and patient as well as future directions for research and management. Currently, there are no similar books on the market that take an all-inclusive, multidisciplinary approach in the care of individuals with Turner syndrome. As such, this book should be the standard of care for management of this challenging and multifaceted condition.

Author: United States. Drug Enforcement Administration. National Training Institute
Publisher:
ISBN:
Category : Government publications
Languages : en
Pages : 38

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Book Description

Author: Takaomi Arai
Publisher: Springer Science & Business Media
ISBN: 4431857095
Category : Science
Languages : en
Pages : 431

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Book Description
Organotin compounds, used as antifouling biocides since 1960, are chemical compounds that act as endocrine disrupters. It is not known how organotin compounds cause hormone disturbance, however, and many questions remain about their effect on aquatic organisms. Studies on organotin compounds have recently evolved, with many new findings reported. Following a worldwide ban on organotin compounds in 2008, alternative compounds will mainly be used, with the potential for coastal areas to become contaminated, causing, among other effects, cholinesterase inhibition in aquatic organisms. Use of alternative compounds must be controlled to avoid such errors. These and other findings are described and concisely summarized in this book, providing a useful reference in countries where alternative biocides are being considered. Included are studies on the effects on marine organisms, making this book an excellent aid to experts in environmental chemistry, to government organizations, and to students.

Author: Bloomsbury Publishing
Publisher: Bloomsbury Publishing
ISBN: 1408102579
Category : Games & Activities
Languages : en
Pages : 719

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Book Description
Anagram Solver is the essential guide to cracking all types of quiz and crossword featuring anagrams. Containing over 200,000 words and phrases, Anagram Solver includes plural noun forms, palindromes, idioms, first names and all parts of speech. Anagrams are grouped by the number of letters they contain with the letters set out in alphabetical order so that once the letters of an anagram are arranged alphabetically, finding the solution is as easy as locating the word in a dictionary.

Author: Benjamin R. Arenkiel
Publisher: Humana Press
ISBN: 9781493919628
Category : Medical
Languages : en
Pages : 0

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Book Description
This volume seeks to familiarize readers with a diverse range of technologies and approaches for probing neuron and circuit architecture, and, when possible, to attach detailed protocols to help guide readers toward practical application. From classical lipophilic dye and conjugated lectin tracing techniques, to electrophysiological, in vivo imaging, viral tract tracing, and emerging genetic methods to mark, manipulate, and monitor neural circuits, Neural Tracing Methods: Tracing Neurons and Their Connections includes reference to an arsenal of tools and technologies currently being implemented in model systems ranging from flies to mice. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Essential and authoritative, Neural Tracing Methods: Tracing Neurons and Their Connections collects a comprehensive compilation of chapters authored by inventors and expert users, that describes state-of-the-art neuronal tracing and functional analysis methods in order to aid researchers in continuing this vital pathway of study.