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Lipid Analysis

Lipid Analysis PDF Author: W. W. Christie
Publisher: Elsevier
ISBN: 0857097865
Category : Technology & Engineering
Languages : en
Pages : 447

Book Description
This well-known and highly successful book was first published in 1973 and has been completely re-written in subsequent editions (published in 1982 and 2003). This new Fourth Edition has become necessary because of the pace of developments in mass spectrometry of intact lipids, which has given recognition of lipid analysis and ‘lipidomics’ as a distinct science. To bring the book up to date with these developments, author William W. Christie is joined by co-author Xianlin Han. Although devoting considerable space to mass spectrometry and lipidomics, Lipid analysis remains a practical guide, in one volume, to the complexities of the analysis of lipids. As in past editions, it is designed to act as a primary source, of value at the laboratory bench rather than residing on a library shelf. Lipid analysis deals with the isolation, separation, identification and structural analysis of glycerolipids, including triacylglycerols, phospholipids, sphingolipids, and the various hydrolysis products of these. The chapters follow a logical sequence from the extraction of lipids to the isolation and characterization of particular lipid classes and of molecular species of each, and to the mass spectrometric analysis of lipids and lipidomics. The new influence of mass spectrometry is due mainly to the development of electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). Most emphasis in this book is placed on ESI, which is enabling structural characterization of different lipid classes and the identification of novel lipids and their molecular species.

Lipid Analysis

Lipid Analysis PDF Author: W. W. Christie
Publisher: Elsevier
ISBN: 0857097865
Category : Technology & Engineering
Languages : en
Pages : 447

Book Description
This well-known and highly successful book was first published in 1973 and has been completely re-written in subsequent editions (published in 1982 and 2003). This new Fourth Edition has become necessary because of the pace of developments in mass spectrometry of intact lipids, which has given recognition of lipid analysis and ‘lipidomics’ as a distinct science. To bring the book up to date with these developments, author William W. Christie is joined by co-author Xianlin Han. Although devoting considerable space to mass spectrometry and lipidomics, Lipid analysis remains a practical guide, in one volume, to the complexities of the analysis of lipids. As in past editions, it is designed to act as a primary source, of value at the laboratory bench rather than residing on a library shelf. Lipid analysis deals with the isolation, separation, identification and structural analysis of glycerolipids, including triacylglycerols, phospholipids, sphingolipids, and the various hydrolysis products of these. The chapters follow a logical sequence from the extraction of lipids to the isolation and characterization of particular lipid classes and of molecular species of each, and to the mass spectrometric analysis of lipids and lipidomics. The new influence of mass spectrometry is due mainly to the development of electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). Most emphasis in this book is placed on ESI, which is enabling structural characterization of different lipid classes and the identification of novel lipids and their molecular species.

Lipid Analysis

Lipid Analysis PDF Author: William W. Christie
Publisher: Elsevier
ISBN: 1483187179
Category : Science
Languages : en
Pages : 353

Book Description
Lipid Analysis: Isolation, Separation, Identification, and Structural Analysis of Lipids focuses on the methods, principles, and approaches involved in the isolation, identification, separation, and structural analysis of lipids. The publication first elaborates on the structure, chemistry and occurrence of lipids, isolation of lipids from tissues, and chromatographic and spectroscopic analysis of lipids. Discussions focus on equipping a laboratory for lipid analysis, spectroscopy, chromatographic procedures, general principles of solvent extraction procedures, structural features of lipids important in analyses, simple and complex lipids, and fatty acids. The text then examines the preparation of volatile derivatives of lipids and analysis of fatty acids and simple lipid classes. Topics include alkyl diglycerides and neutral plasmalogens, analysis of the hydrolysis products of simple lipids, preparation of large quantities of pure fatty acids, physical characterization of fatty acids, location of double bonds in fatty acid chains, and saponification of lipids. The manuscript covers the analysis and radioassay of isotopically-labelled lipids, enzymatic hydrolysis of lipids, and analysis of molecular species of lipids. The publication is a primary reference for researchers interested in lipid analysis.

Lipid Analysis in Oils and Fats

Lipid Analysis in Oils and Fats PDF Author: R.J. Hamilton
Publisher: Springer Science & Business Media
ISBN: 1461311314
Category : Technology & Engineering
Languages : en
Pages : 406

Book Description
This book focuses on the developments in the field of lipid analysis, providing an up-to-date review of the analytical techniques available to chemists and technologists to identify complex molecules. The requisite theoretical background will be provided for individual techniques, together with their strengths and weaknesses, and a guide to the enormous range of commercial applications. It will be an invaluable reference source to all sectors of the oils and fats industry where accurate labeling of foods, food contamination and adulteration are issues of increasing interest and concern.

Lipidomics and Bioactive Lipids: Mass Spectrometry Based Lipid Analysis

Lipidomics and Bioactive Lipids: Mass Spectrometry Based Lipid Analysis PDF Author:
Publisher: Elsevier
ISBN: 0080554881
Category : Science
Languages : en
Pages : 432

Book Description
This volume in the well-established Methods in Enzymology series features methods for the study of lipids using mass spectrometry techniques. Articles in this volume cover topics such as Qualitative Analysis and Quantitative Assessment of Changes in Neutral Glycerol Lipid Molecular Species within Cells; Glycerophospholipid identification and quantitation by electrospray ionization mass spectrometry; Detection and Quantitation of Eicosanoids via High Performance Liquid Chromatography/Electrospray Ionization Mass Spectrometry; Structure-specific, quantitative methods for "lipidomic" analysis of sphingolipids by tandem mass spectrometry; Analysis of Ubiquinones, Dolichols and Dolichol Diphosphate-Oligosaccharides by Liquid Chromatography Electrospray Ionization Mass Spectrometry; Extraction and Analysis of Sterols in Biological Matrices by High-Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry; The Lipid Maps Initiative in Lipidomics; Basic analytical systems for lipidomics by mass spectrometry in Japan; The European Lipidomics Initiative Enabling technologies; Lipidomic analysis of Signaling Pathways; Bioinformatics for Lipidomics; Mediator Lipidomics: Search Algorithms for Eicosanoids, Resolvins and Protectins; A guide to biochemical systems modeling of sphingolipids for the biochemist; and Quantitation and Standardization of Lipid Internal Standards for Mass Spectroscopy.

Advances in Lipid Methodology

Advances in Lipid Methodology PDF Author: W. W. Christie
Publisher: Elsevier
ISBN: 0857097997
Category : Science
Languages : en
Pages : 305

Book Description
This is the fourth volume of an occasional series of review volumes dealing with aspects of lipid methodology. As with the first three volumes, topics have been selected that have been developing rapidly in recent years and have some importance to lipid analysis. The authors are all leading international experts.Topics covered include: analysis of plant lipoxygenase metabolites, preparative high-performance liquid chromatography of lipids, structural analysis of fatty acids, and analysis of stable isotopes in lipids, among others.

Diet and Health

Diet and Health PDF Author: National Research Council
Publisher: National Academies Press
ISBN: 0309039940
Category : Medical
Languages : en
Pages : 765

Book Description
Diet and Health examines the many complex issues concerning diet and its role in increasing or decreasing the risk of chronic disease. It proposes dietary recommendations for reducing the risk of the major diseases and causes of death today: atherosclerotic cardiovascular diseases (including heart attack and stroke), cancer, high blood pressure, obesity, osteoporosis, diabetes mellitus, liver disease, and dental caries.

High Performance Liquid Chromatography in Plant Sciences

High Performance Liquid Chromatography in Plant Sciences PDF Author: Hans-Ferdinand Linskens
Publisher: Springer Science & Business Media
ISBN: 3642829511
Category : Science
Languages : en
Pages : 267

Book Description
Modern Methods of Plant Analysis When the handbook Modern Methods of Plant Analysis was first introduced in 1954 the considerations were: 1. the dependence of scientific progress in biology on the improvement of existing and the introduction of new methods; 2. the difficulty in finding many new analytical methods in specialized journals which are normally not accessible to experimental plant biologists; 3. the fact that in the methods sections of papers the description of methods is frequently so compact, or even sometimes so incomplete that it is difficult to reproduce experiments. These considerations still stand today. The series was highly successful, seven volumes appearing between 1956 and 1964. Since there is still today a demand for the old series, the publisher has decided to resume publication of Modern Methods of Plant Analysis. It is hoped that the New Series will be just as acceptable to those working in plant sciences and related fields as the early volumes undoubtedly were. It is difficult to single out the major reasons for success of any publication, but we believe that the methods published in the first series were up-to-date at the time and presented in a way that made description, as applied to plant material, complete in itselfwith little need to consult other publications. Contributing authors have attempted to follow these guidelines in this New Series of volumes.

Lipids in Freshwater Ecosystems

Lipids in Freshwater Ecosystems PDF Author: Michael T. Arts
Publisher: Springer Science & Business Media
ISBN: 1461205476
Category : Science
Languages : en
Pages : 333

Book Description
The fundamental purpose of this book is to synthesise the divergent literature on aquatic lipids into a co-ordinated, digestible form. A large part of the book addresses lipid composition and production in freshwater organisms, with chapters on phytoplankton, zooplankton and benthic invertebrates. A common theme throughout the book is the function of lipids in aquatic food webs, with a chapter devoted exclusively to lipids as indicators of health in fish populations. A complementary chapter highlights the role of lipids and essential fatty acids in mariculture. Methodologies to determine the lipid content of aquatic samples and suggestions as to the utility of fatty acids as trophic markers are included, as is one chapter on the role of lipids in the bioaccumulation and bioconcentration of toxicants and another on the relationships between lipids and surface films and foams. The final chapter highlights the similarities and differences between lipids of marine and freshwater origin. Students and researchers in ecology, phycology, aquatic toxicology, physiological ecology and limnology will find this an invaluable guide and reference.

Ionic Liquids in Lipid Processing and Analysis

Ionic Liquids in Lipid Processing and Analysis PDF Author: Xuebing Xu
Publisher: Elsevier
ISBN: 1630670480
Category : Science
Languages : en
Pages : 486

Book Description
This book serves as a reference for those interested in state-of-the-art research on the science and technology of ionic liquids (ILs), particularly in relation to lipids processing and analysis. Topics include a review of the chemistry and physics of ILs as well as a quantitative understanding of structure-activity relationships at the molecular level. Further, chapter authors examine the molecular basis of the toxicity of ILs, the prediction of the properties of ILs, and the rationale and steps toward a priori design of ionic liquids for task-defined applications. Emerging research in developing lipid-inspired ILs and their prospective use in drug formulation is described. Among the highlights are the latest advances in IL-mediated biocatalysis and biotransformation, along with lipase production, purification, and activation. - Reviews the state-of-the-art applications of ionic liquids in lipid processing and relevant areas from a variety of perspectives - Summarizes the latest advances in the measurement of the physical and chemical properties of ionic liquids and available databases of thermodynamic property datapoints - Presents the tremendous opportunities provided and challenges faced from ionic liquids as a newly emerging technology for lipids processing area

Lipid Analysis

Lipid Analysis PDF Author: Prof F W Hemming
Publisher: Garland Science
ISBN: 1000102548
Category : Science
Languages : en
Pages : 189

Book Description
There is increasing recognition of the diversity and biological importance of lipids. Lipid modifications of other biological molecules are now also the subject of intense research activity. This 'user-friendly' introduction describes the techniques currently in use, with many clear figures and line drawings to illustrate separations.