Analysis of Fungal Inteins PDF Download

Author: Annika Anna Maria Bokor
Publisher:
ISBN:
Category : DNA.
Languages : en
Pages : 596

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Author: Sumana Islam
Publisher:
ISBN:
Category : Aspergillus nidulans
Languages : en
Pages : 766

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Author: Miquel Vila-Perelló
Publisher: Humana
ISBN: 9781071604335
Category : Science
Languages : en
Pages : 0

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Book Description
This book provides a comprehensive overview of Expressed Protein Ligation (EPL), detailing methods and protocols to generate site-specifically modified proteins. Chapters include an overview of the protein semi-synthesis field, as well as related areas that have contributed to the development of EPL such as protein splicing and peptide synthesis. Following the introductory chapters, the rest of the book guides readers through protocols to perform EPL reactions, methods to synthesize peptide thioesters and to perform peptide and protein ligations, label proteins inside living cells, protocols for the semi-synthesis of phorphorylated, glycosylated and ubiquitylated proteins, synthesis and assembly of assymetrically modified nucleosomes, use of ligation auxiliaries and synthesis of cyclic proteins, as well as novel desulfurization strategies and use of selective Cys side chain protection to obtain precisely modified proteins.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Expressed Protein Ligation: Methods and Protocols will ensure successful implementation of protein semi-synthesis methods to further study the structure and function of proteins.

Author:
Publisher: Academic Press
ISBN: 0128191295
Category : Science
Languages : en
Pages : 310

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Book Description
Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part C, Volume 633, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial. This release includes sections on Next generation probes for molecular imaging in cells, Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluorescein, Converting avidin to bind ligands other than biotin, especially steroids, Chemoenzymatic Labeling Strategy, Engineered Siderophores, Small molecules to inhibit bacterial population behavior, NMR tube bioreactor, Small molecule controlled RAS activation system, Small molecule regulated Cas9, the Design and application of synthetic receptors, and much more. - Contains the authority of authors who are leaders in their field - Provides a comprehensive source on new methods and research in enzymology

Author: Alan J. Barrett
Publisher: Elsevier
ISBN: 0080984150
Category : Science
Languages : en
Pages : 1182

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Handbook of Proteolytic Enzymes, Second Edition, Volume 1: Aspartic and Metallo Peptidases is a compilation of numerous progressive research studies on proteolytic enzymes. This edition is organized into two main sections encompassing 328 chapters. This handbook is organized around a system for the classification of peptidases, which is a hierarchical one built on the concepts of catalytic type, clan, family and peptidase. The concept of catalytic type of a peptidase depends upon the chemical nature of the groups responsible for catalysis. The recognized catalytic types are aspartic, cysteine, metallo, serine, threonine, and the unclassified enzymes, while clans and families are groups of homologous peptidases. Homology at the level of a family of peptidases is shown by statistically significant relationship in amino acid sequence to a representative member called the type example, or to another member of the family that has already been shown to be related to the type example. Each chapter discusses the history, activity, specificity, structural chemistry, preparation, and biological aspects of the enzyme. This book will prove useful to enzyme chemists and researchers.

Author: Sabine Fillinger
Publisher: Springer
ISBN: 3319233718
Category : Science
Languages : en
Pages : 488

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The fungal genus Botrytis is the focus of intensive scientific research worldwide. The complex interactions between this pathogen and the plants it infects and the economic importance of the diseases caused by Botrytis (principally grey mould) on more than 1400 species of cultivated plants pre- and post-harvest, render this pathogen of particular interest to farmers, advisers, students and researchers in many fields worldwide. This 20-chapter book is a comprehensive treatise covering the rapidly developing science of Botrytis and reflecting the major developments in studies of this fungus. It will serve as a source of general information for specialists in agriculture and horticulture, and also for students and scientists interested in the biology of this fascinating, multifaceted phytopathogenic fungal species.

Author: Joseph Heitman
Publisher: John Wiley & Sons
ISBN: 1555819583
Category : Medical
Languages : en
Pages : 1136

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Book Description
Fungi research and knowledge grew rapidly following recent advances in genetics and genomics. This book synthesizes new knowledge with existing information to stimulate new scientific questions and propel fungal scientists on to the next stages of research. This book is a comprehensive guide on fungi, environmental sensing, genetics, genomics, interactions with microbes, plants, insects, and humans, technological applications, and natural product development.

Author: Barry S. C. Leadbeater
Publisher: Cambridge University Press
ISBN: 0521884446
Category : Science
Languages : en
Pages : 331

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Book Description
A unique account of the biology, ecology and evolution of choanoflagellates - the closest, known, living, unicellular relatives of animals.

Author: Marlene Belfort
Publisher: Springer Science & Business Media
ISBN: 9783540852353
Category : Science
Languages : en
Pages : 404

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Book Description
This book provides the first and only comprehensive description and detailed summary of the genetics, structure, function, mechanisms of action, evolution and engineering of homing endonucleases and inteins. These two unique protein superfamilies, which are tied together through their frequent fusion and coevolution, have generated considerable excitement for their fundamental, structural, and functional properties, their evolution as parasitic elements, and their widespread applications as gene targeting agents and as instruments for the generation of modified proteins and novel protein combinations.

Author: Ashraf Brik
Publisher: John Wiley & Sons
ISBN: 3527346600
Category : Science
Languages : en
Pages : 626

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Book Description
How to synthesize native and modified proteins in the test tube With contributions from a panel of experts representing a range of disciplines, Total Chemical Synthesis of Proteins presents a carefully curated collection of synthetic approaches and strategies for the total synthesis of native and modified proteins. Comprehensive in scope, this important reference explores the three main chemoselective ligation methods for assembling unprotected peptide segments, including native chemical ligation (NCL). It includes information on synthetic strategies for the complex polypeptides that constitute glycoproteins, sulfoproteins, and membrane proteins, as well as their characterization. In addition, important areas of application for total protein synthesis are detailed, such as protein crystallography, protein engineering, and biomedical research. The authors also discuss the synthetic challenges that remain to be addressed. This unmatched resource: Contains valuable insights from the pioneers in the field of chemical protein synthesis Presents proven synthetic approaches for a range of protein families Explores key applications of precisely controlled protein synthesis, including novel diagnostics and therapeutics Written for organic chemists, biochemists, biotechnologists, and molecular biologists, Total Chemical Synthesis of Proteins provides key knowledge for everyone venturing into the burgeoning field of protein design and synthetic biology.