An Immunocapture\reverse Transcription\polymerase Chain Reaction Technique for Detection and Molecular Characterization of Bean Common Mosaic Virus in Mixed Infections PDF Download

Author: Roshan Abdallah
Publisher:
ISBN:
Category : Bean common mosaic virus
Languages : en
Pages : 228

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Book Description

Author: Ling Xu
Publisher:
ISBN:
Category : Bean common mosaic virus
Languages : en
Pages : 110

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Book Description
Polyclonal antisera were raised against isolates of bean common mosaic virus (BCMV) and bean common mosaic necrosis virus (BCMNV) using conventional serological methods. Infected tissues containing, respectively, 22 recognized BCMV and BCMNV isolates were tested against the two antisera by antigen-coated plate (ACP) ELISA and double antibody sandwich (DAS) ELISA. Results indicated that each immunoglobulin was virus-specific by DAS-ELISA, providing clear distinction between BCMV and BCMNV. A reverse transcription, polymerase chain reaction (RT-PCR)-based assay in combination with restriction endonuclease analyses, was developed for molecular detection of BCMV, BCMNV and their pathogroups. Specific detection of the two viruses was accomplished by constructing two virus-specific primer pairs that amplified a PCR product specific for each virus. Distinction of two BCMNV pathogroups (PG-III and PG-VI) was achieved by restriction enzyme XbaI digestion of BCMNV PCR products. However, none of the tested restriction enzymes clearly differentiated the five recognized BCMV pathogroups. A primer pair Dts/Uny15 specific for BCMV pathogroup V was also developed. By its RT-PCR application, four BCMV-PG-V isolates were differentiated from the other known variants of BCMV pathogroup I, II, IV and VII. Thus, by a combination of RT-PCR and restriction enzyme analyses, it was possible to differentiate both viruses, and two pathogroups of BCMNV, and one pathogroup of BCMV.

Author: Baozhong Meng
Publisher: Springer
ISBN: 3319577069
Category : Science
Languages : en
Pages : 688

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Book Description
The domestication of grapes dates back five thousand years ago and has spread to nearly all continents. In recent years, grape acreage has increased dramatically in new regions, including the United States of America, Chile, Asia (China and India), and Turkey. A major limiting factor to the sustained production of premium grapes and wines is infections by viruses. The advent of powerful molecular and metagenomics technologies, such as molecular cloning and next generation sequencing, allowed the discovery of new viruses from grapes. To date, grapevine is susceptible to 64 viruses that belong to highly diverse taxonomic groups. The most damaging diseases include: (1) infectious degeneration; (2) leafroll disease complex; and (3) rugose wood complex. Recently, two new disease syndromes have been recognized: Syrah decline and red blotch. Losses due to fanleaf degeneration are estimated at $1 billion annually in France alone. Other diseases including leafroll, rugose wood, Syrah de cline and red blotch can result in total crop loss several years post-infection. This situation is further exacerbated by mixed infections with multiple viruses and other biotic as well as adverse abiotic environmental conditions, such as drought and winter damage, causing even greater destruction. The book builds upon the last handbook (written over twenty years ago) on the part of diagnostics and extensively expands its scope by inclusion of molecular biology aspects of select viruses that are widespread and economically most important. This includes most current information on the biology, transmission, genome replication, transcription, subcellular localization, as well as virus-host interactions. It also touches on several novel areas of scientific inquiry. It also contains suggested directions for future research in the field of grapevine virology.

Author: K. Subramanya Sastry
Publisher: Springer Science & Business Media
ISBN: 8132208137
Category : Medical
Languages : en
Pages : 343

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Book Description
Seeds provide an efficient means in disseminating plant virus and viroid diseases. The success of modern agriculture depends on pathogen free seed with high yielding character and in turn disease management. There is a serious scientific concern about the transmission of plant viruses sexually through seed and asexually through plant propagules. The present book provides the latest information along with the total list of seed transmitted virus and viroid diseases at global level including, the yield losses, diagnostic techniques, mechanism of seed transmission, epidemiology and virus disease management aspects. Additional information is also provided on the transmission of plant virus and virus-like diseases through vegetative propagules. It is also well known that seed transmitted viruses are introduced into new countries and continents during large-scale traffic movements through infected germplasm and plant propogules. The latest diagnostic molecular techniques in different virus-host combinations along with disease management measures have been included. The book shall be a good reference source and also a text book to the research scientists, teachers, students of plant pathology, agriculture, horticulture, life sciences, green house managers, professional entrepreneurs, persons involved in quarantines and seed companies. This book has several important features of seed transmitted virus diseases and is a good informative source and thus deserves a place in almost all university libraries, seed companies and research organizations.

Author: Bikash Mandal
Publisher: Springer
ISBN: 9811056722
Category : Science
Languages : en
Pages : 800

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Book Description
The book is a compilation of research work carried out on plant viruses during past 100 years in India. Plant viruses are important constraints in Indian agriculture. Tropical and sub-tropical environments and intensive crop cultivation practices ideally favours perpetuation of numerous plant viruses and their vectors in India, which often cause wide spread crop losses. Of all the plant pathogens, studies of plant viruses have received a special attention as they are difficult to manage. A large body of literature has been published on the plant virus research from India during past 100 years; however the information is so far not available in one place. This book provides comprehensive information on the biology, molecular biology, epidemics, crop losses, diagnosis and management of viruses and viroids occurring in India. Description of properties of the viruses are provided in the chapters comprising of different genera such as Allexivirus, Begomovirus, Babuvirus, Badnavirus, Carlavirus, Carmovirus, Cucumovirus, Closterovirus, Ilavirus, Mandrivirus, Potyvirus, Tospovirus, Tungrovirus and Sobemovirus. Virus-vector research related to aphid, thrips and whitefly is discussed. The work on the management aspects of plant viral diseases has been described with reference to the conventional, antiviral and transgenic approaches. Further, the quarantine mechanism developed in India for the exclusion of viruses and vectors has also been included. The book also provides useful information about the capacity building on the research and education on Plant Virology in India. Overall, the book covers a wide range of accounts of research findings and innovations in Plant Virology in India during past 100 years. The book will be a resourceful reference to the students, scientists, agricultural professionals and policy makers.

Author: Paul Neergaard
Publisher:
ISBN:
Category : Pathology
Languages : en
Pages : 878

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Book Description
V.1: Pathognes-diseases-hosts; Mechanisms of seed transmission; Principles of control; Seed health testing methods; Assessment of seed-borne inoculum. v. 2: Pathogens-diseases-hosts; Mechanisms of seed transmission; Principles of control; Seed health testing methods; Assessment of seed-borne inoculum.

Author: K. Subramanya Sastry
Publisher: Springer
ISBN: 9788132239130
Category : Science
Languages : en
Pages : 2936

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Book Description
The Encyclopedia of Plant Viruses and Viroids provides an up-to-date information on the viruses and viroids infecting all types of cultivated and weed plants at global level; and is unique among plant virology texts as it is organized alphabetically by the genus name of the host plant infected. It allows the reader to readily determine all of the different viruses currently reported naturally infecting the plant genus and species. Information is provided for each virus and viroids on common synonyms, current taxonomic status, geographical distribution, symptoms induced, other known hosts, means of transmission and properties of both the virus particles and the genome. Where ever the same virus is known to infect multiple hosts, host-specific information, biological properties and genome characters are presented under each host affected. The index can be utilized to identify other crops infected by the same virus, showing which other crops might be at risk of infection in the event of introduction of a virus that has not previously reported in an area, or which might serve as potential virus reservoirs for infection of more sensitive or economically important crops than the host in which it is initially identified. The taxonomy and nomenclature of the viruses and viroids are followed based on the current guidelines of the 10th ICTV Report, covering up to 2018 and also about many new viruses and viroids that have been reported but not yet recognized as species by ICTV, have been included in this Encyclopedia. The uniqueness of this Encyclopedia is that all the known viruses and viroids affecting more than 1010 plant species at global level are described and the plant species are arranged in alphabetical order of the scientific name of the plant along the relevant information on 1518 viruses and viroids and is the ready-reckoner of the global plant species and their viruses and viroids for students, scientists, teachers of Plant Pathology& Virology; and also for the crop protection professionals, agricultural policymakers, seed companies and quarantine agencies.

Author: R. Z. Sayyed
Publisher: Springer
ISBN: 9811367906
Category : Technology & Engineering
Languages : en
Pages : 334

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Book Description
Sustainable increase in agricultural production while keeping the environmental quality, agro-ecosystem function and biodiversity is a real challenge in current agricultural practices. Application of PGPR can help in meeting the expected demand for increasing agricultural productivity to feed the world’s booming population. Global concern over the demerits of chemicals in agriculture has diverted the attention of researchers towards sustainable agriculture by utilizing the potential of Plant Growth Promoting Rhizobacteria (PGPR). Use of PGPR as biofertilizers, biopesticides, soil, and plant health managers has gained considerable agricultural and commercial significance. The book Plant Growth Promoting Rhizobacteria (PGPR): Prospects for Sustainable Agriculture has contributions in the form of book chapter from 25 eminent global researchers, that discusses about the PGPRs and their role in growth promotion of various crop plants, suppression of wide range of phytopathogens, their formulation, effect of various factors on growth and performance of PGPR, assessment of diversity of PGPR through microsatellites and role of PGPR in mitigating biotic and abiotic stress.This book will be helpful for students, teachers, researchers, and entrepreneurs involved in PGPR and allied fields. The book will be highly useful to researchers, teachers, students, entrepreneurs, and policymakers.

Author: Muhamed Brka
Publisher: Springer Nature
ISBN: 3030400492
Category : Technology & Engineering
Languages : en
Pages : 377

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Book Description
This book gathers the proceedings of the 30th Scientific-Experts Conference of Agriculture and Food Industry, held on September 26-27, 2019, in Sarajevo, Bosnia and Herzegovina. It reports on the application of innovative technologies in food sciences and agriculture, and covers research in plant and animal production, agricultural economics and food production. Further, the book discusses key social and environmental issues, and proposes answers to current challenges. The conference was jointly organized by the Faculty of Agriculture and Food Sciences of the University of Sarajevo, Bosnia and Herzegovina, the Faculty of Agriculture of Ege University, Turkey, the Bosnia and Herzegovina Medical and Biological Engineering Society, and the Faculty of Agriculture of the University of Belgrade, Serbia. The proceedings offer a timely snapshot of cutting-edge, multidisciplinary research and developments in modern agriculture. As such, they address the needs of researchers and professionals, agricultural companies, food producers, and regulatory and food safety agencies.

Author: Ravindra Kumar
Publisher: Springer Nature
ISBN: 9813290463
Category : Technology & Engineering
Languages : en
Pages : 860

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Book Description
The global population is increasing rapidly, and feeding the ever-increasing population poses a serious challenge for agriculturalists around the world. Seed is a basic and critical input in agriculture to ensure global food security. Roughly 90 percent of the crops grown all over the world are propagated by seed. However, seed can also harbour and spread pathogens, e.g. fungi, bacteria, nematodes, viruses etc., which cause devastating diseases. Seed-borne pathogens represent a major threat to crop establishment and yield. Hence, timely detection and diagnosis is a prerequisite for their effective management. The book "Seed-Borne Diseases of Agricultural Crops: Detection, Diagnosis & Management" addresses key issues related to seed-borne/transmitted diseases in various agricultural crops. Divided into 30 chapters, it offers a comprehensive compilation of papers concerning: the history of seed pathology, importance of seed-borne diseases, seed-borne diseases and quarantine, seed health testing and certification, detection and diagnosis of seed-borne diseases and their phytopathogens, host-parasite interactions during development of seed-borne diseases, diversity of seed-borne pathogens, seed-borne diseases in major agricultural crops, non-parasitic seed disorders, mechanisms of seed transmission and seed infection, storage fungi and mycotoxins, impact of seed-borne diseases on human and animal health, and management options for seed-borne diseases. We wish to thank all of the eminent researchers who contributed valuable chapters to our book, which will be immensely useful for students, researchers, academics, and all those involved in various agro-industries.