Amine Oxidases and Methods for Their Study PDF Download

Author: R. Kapeller-Adler
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 344

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Author: V. Z. Gorkin
Publisher: Elsevier
ISBN: 148316165X
Category : Science
Languages : en
Pages : 291

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Book Description
Amine Oxidases in Clinical Research discusses the functions of amine oxidases in the rapid development of medical and biological research. Composed of 13 chapters, the book focuses on amine oxidases, including their key role in the metabolism of biogenic amines and other nitrogenous compounds in diseases and human health. The book considers primarily the enzymes denoted as EC 1.4.3.4 (flavin amine oxidases or monoamine oxidases) and EC 1.4.3.6 (copper-containing amine oxidase or diamine oxidases). To measure the estimates of amine oxidase activity, several methods and a number of requirements are presented. A description of the distribution and intracellular localization of amine oxidase follows. The methods of isolation and purification of amine oxides from different sources and the results of these various methods are explained. The text also considers the properties of the purified amine oxidases: the different methods of isolation and purification; storage conditions; and analytical techniques. Care is given when examining amine oxidases, because these may exhibit considerable differences in substrate specificity even if their physicochemical properties are similar. Inhibition of amine oxidase activity by unspecific and specific inhibitions is further discussed, including the biological functions and known effects of hormones on the biosynthesis and catabolism of the amine oxidase. Finally, the possible pathogenetic and/or diagnostic significance of the amine oxidases, as well as the corrective approaches seen in pathological conditions and in clinical medicine, are explained. Written as a handbook for the medically oriented investigators, biologists, and medical and chemical researchers, this book will prove a handy reference.

Author: Mondovi
Publisher: CRC Press
ISBN: 1351085409
Category : Medical
Languages : en
Pages : 299

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Book Description
A good portion of this book has been devoted to the copper-dependent enzymes, these being the more numerous. The chapter dealing with serum amine oxidases also focuses attention on their catalytic mechanism, as these enzymes have been studied in greater depth.As the presentation of topics whose experimental basis is rapidly developing is likely to stimulate the reader‘s interest, many bibliographic references have been included.Readers could find this book poor, as far as many topics are dealt with in a relatively little space, but we believe it essential to trace the background of our present knowledge in the field of amine oxidases, stressing the future outlook of research on these enzymes, for they are becoming more and more important in general and medical biochemistry.

Author: Claudia Binda
Publisher: Springer Nature
ISBN: 1071626434
Category : Medical
Languages : en
Pages : 259

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Book Description
This detailed book examines the main methods to study mammalian monoamine oxidases (MAOs), ranging from cell biology to computational chemistry. Beginning with techniques on how to obtain pure samples of MAO A and MAO B, the volume continues by covering assays and techniques used to measure MAO enzymatic activity and perform inhibition studies, methods to address cellular localization and function of MAOs, either in cell lines or in animal models, as well as computational methods applied to rational drug design approaches that are used to develop new MAO inhibitors. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Monoamine Oxidase: Methods and Protocols serves as a vital resource for scientists who are interested in studying MAOs and other similar amine oxidase enzymes.

Author: M. C. Boadle
Publisher:
ISBN:
Category : Amine oxidase
Languages : en
Pages : 0

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Book Description

Author: Mondovi
Publisher: CRC Press
ISBN: 9781315897851
Category :
Languages : en
Pages : 299

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Book Description
A good portion of this book has been devoted to the copper-dependent enzymes, these being the more numerous. The chapter dealing with serum amine oxidases also focuses attention on their catalytic mechanism, as these enzymes have been studied in greater depth.As the presentation of topics whose experimental basis is rapidly developing is likely to stimulate the reader�s interest, many bibliographic references have been included.Readers could find this book poor, as far as many topics are dealt with in a relatively little space, but we believe it essential to trace the background of our present knowledge in the field of amine oxidases, stressing the future outlook of research on these enzymes, for they are becoming more and more important in general and medical biochemistry.

Author: Gonzalo de Gonzalo
Publisher: John Wiley & Sons
ISBN: 352734683X
Category : Science
Languages : en
Pages : 54

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Book Description
This reference book originates from the interdisciplinary research cooperation between academia and industry. In three distinct parts, latest results from basic research on stable enzymes are explained and brought into context with possible industrial applications. Downstream processing technology as well as biocatalytic and biotechnological production processes from global players display the enormous potential of biocatalysts. Application of "extreme" reaction conditions (i.e. unconventional, such as high temperature, pressure, and pH value) - biocatalysts are normally used within a well defined process window - leads to novel synthetic effects. Both novel enzyme systems and the synthetic routes in which they can be applied are made accessible to the reader. In addition, the complementary innovative process technology under unconventional conditions is highlighted by latest examples from biotech industry.

Author: Corinna R. Hess
Publisher:
ISBN:
Category : Amine oxidase
Languages : en
Pages : 194

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Book Description
Amine oxidases (AO) are copper-containing enzymes that catalyze the conversion of amines to aldehydes. The active site is deeply buried within the protein network, a factor which has precluded many investigations into the mechanism. A series of electron tunneling molecular wires has been synthesized, designed to access the cofactors of amine oxidase by means of the substrate channel. These wires are comprised of phenyl-ethynyl units and thus promote rapid rates of electron transfer to the active site. An amine group attached to one end of the oligomer serves as the channel-specific functionality, which binds within the channel. Binding of the oligomers to AO was demonstrated through inhibition and fluorescence quenching studies. A variety of reporter groups has been attached to the chain end opposite the amine group, allowing us to probe the enzyme using a variety of techniques. A Re terminated complex was designed for electron transfer studies with the enzyme. The photophysics of this complex were investigated in the presence of AO. A thiol terminated wire also has been synthesized, which was employed in electrochemical studies of the amine oxidase cofactors. These studies provide evidence for the ability to communicate with the active site using the molecular wires. In addition, these complexes fulfilled our primary goal and enabled us to determine a redox potential of the TPQ cofactor.

Author:
Publisher: Academic Press
ISBN: 012820138X
Category : Science
Languages : en
Pages : 568

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Book Description
The Enzymes, Volume 47, highlights new advances in the field, with this new volume presenting interesting chapters on The Multipurpose Family of Oxidases, Vanillyl alcohol oxidase, Choline oxidases, Aryl alcohol oxidase, D- and L-amino acid oxidases, Sugar oxidases, Phenolic Compounds hydroxylases, Baeyer-Villiger Monooxygenases, Flavin-dependent halogenases, Flavin-dependent dehalogenases, Styrene Monooxygenases, Bacterial luciferases, Cellobiose Dehydrogenases, Prenylated flavoenzymes, Ene-reductases, Flavoenzymes in Biocatalysis. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in The Enzymes series

Author: Fernanda Duarte
Publisher: John Wiley & Sons
ISBN: 1119245397
Category : Science
Languages : en
Pages : 139

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A comprehensive overview of current empirical valence bond (EVB) theory and applications, one of the most powerful tools for studying chemical processes in the condensed phase and in enzymes. Discusses the application of EVB models to a broad range of molecular systems of chemical and biological interest, including reaction dynamics, design of artificial catalysts, and the study of complex biological problems Edited by a rising star in the field of computational enzymology Foreword by Nobel laureate Arieh Warshel, who first developed the EVB approach