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Author: Pierre Taberlet Publisher: Oxford University Press ISBN: 0191079995 Category : Science Languages : en Pages : 272
Book Description
Environmental DNA (eDNA) refers to DNA that can be extracted from environmental samples (such as soil, water, feces, or air) without the prior isolation of any target organism. The analysis of environmental DNA has the potential of providing high-throughput information on taxa and functional genes in a given environment, and is easily amenable to the study of both aquatic and terrestrial ecosystems. It can provide an understanding of past or present biological communities as well as their trophic relationships, and can thus offer useful insights into ecosystem functioning. There is now a rapidly-growing interest amongst biologists in applying analysis of environmental DNA to their own research. However, good practices and protocols dealing with environmental DNA are currently widely dispersed across numerous papers, with many of them presenting only preliminary results and using a diversity of methods. In this context, the principal objective of this practical handbook is to provide biologists (both students and researchers) with the scientific background necessary to assist with the understanding and implementation of best practices and analyses based on environmental DNA.
Author: Pierre Taberlet Publisher: Oxford University Press ISBN: 0191079995 Category : Science Languages : en Pages : 272
Book Description
Environmental DNA (eDNA) refers to DNA that can be extracted from environmental samples (such as soil, water, feces, or air) without the prior isolation of any target organism. The analysis of environmental DNA has the potential of providing high-throughput information on taxa and functional genes in a given environment, and is easily amenable to the study of both aquatic and terrestrial ecosystems. It can provide an understanding of past or present biological communities as well as their trophic relationships, and can thus offer useful insights into ecosystem functioning. There is now a rapidly-growing interest amongst biologists in applying analysis of environmental DNA to their own research. However, good practices and protocols dealing with environmental DNA are currently widely dispersed across numerous papers, with many of them presenting only preliminary results and using a diversity of methods. In this context, the principal objective of this practical handbook is to provide biologists (both students and researchers) with the scientific background necessary to assist with the understanding and implementation of best practices and analyses based on environmental DNA.
Author: Jerney, Jacqueline Publisher: Nordic Council of Ministers ISBN: 9289375191 Category : Science Languages : en Pages : 68
Book Description
We present guidelines for using environmental DNA metabarcoding, together with conventional techniques, to monitor the taxonomic diversity of phytoplankton in marine and brackish waters. The focus is on eukaryotic and prokaryotic phytoplankton using 18S and 16S rRNA primers and high-throughput sequencing. Information and recommendations on sampling, sample processing, molecular biological work, quality control, bioinformatics, data storage and management and cost estimates are included so that the method can be used to complement standardized light microscopy. A scientific literature review, discussion on future perspectives, reference databases and standardization are included. Using eDNA metabarcoding to complement standardized light microscopy advances conventional monitoring and research on phytoplankton communities to assess biodiversity and the state of the marine environment.
Author: Jeremy Barnes Publisher: Mountaineers Books ISBN: 1680510789 Category : Sports & Recreation Languages : en Pages : 481
Book Description
100 hikes in the Alpine Lakes Wilderness, including the newly protected Middle Fork Snoqualmie Valley Mix of day hikes and classic backpacking routes Stunning, oversized full-color guide The lush Alpine Lakes Wilderness in Washington’s Central Cascades contains a plethora of trails, rugged glacier-carved mountains, and more than 700 sparkling alpine lakes and ponds. Accessed via nearly 50 trailheads, more than 600 miles of trails offer hikers leisurely strolls along wooded creeks, climbs up mountain passes, or lunch spots next to glassy tarns. It is one of the most popular and beloved places for hikers in this region. The all-new guide, Alpine Lakes Wilderness, features a wide range of hikes that vary in difficulty, geography, and theme so that hikers of any age and skill level will find trails that fit their taste. Easy to use, the guide includes details on overnight permits, car-camping options near wilderness access points, detailed maps, elevation gain/ loss, and turn-by-turn mileage and directions. Interesting historical background and natural history round out the trail descriptions.
Author: Mary Daheim Publisher: Ballantine Books ISBN: 0345468155 Category : Fiction Languages : en Pages : 335
Book Description
For many mystery readers, Alpine, Washington–Mary Daheim’s fictional small town in the foothills of the Cascade Mountains–has become a beloved second home, a delicious retreat from the stresses of life. Yet the editor of The Alpine Advocate, Emma Lord, knows all too well that the picturesque old logging town is loaded with scandal: family feuds, illicit romance, chicanery, and sometimes deadly violence. THE ALPINE RECLUSE In the middle of a hot midsummer night, Emma is awakened by fire trucks rushing to a blaze at the nearby home of newlyweds Tim and Tiffany Rafferty. At daybreak, Tiffany and her unborn child are safe, but Tim, never blessed with good luck in all his thirty-plus years, has perished in the fierce conflagration. Sheriff Milo Dodge suspects murder and arson, and rumors fly from the Burger Barn and Mugs Ahoy to the Grocery Basket and the Venison Inn. Some swear the Rafferty marriage was crumbling. Others hint at stock fraud. A few mention momentary sightings of a possibly mad recluse known as Old Nick. Sacrificing the heady enticements of a budding romance to nail down a great story, Emma shifts into high investigative gear while her fearless House & Home editor, Vida Runkel, rushes in where angels fear to tread: straight into the private lives of some of Alpine’s most respectable–and now terminally edgy–citizens. But neither Emma nor Vida suspects the unbelievable truth.
Author: Pierre Taberlet Publisher: Oxford University Press ISBN: 0198767226 Category : Medical Languages : en Pages : 268
Book Description
Environmental DNA (eDNA) refers to DNA that can be extracted from environmental samples (such as soil, water, feces, or air) without the prior isolation of any target organism. The analysis of environmental DNA has the potential of providing high-throughput information on taxa and functional genes in a given environment, and is easily amenable to the study of both aquatic and terrestrial ecosystems. It can provide an understanding of past or present biological communities as well as their trophic relationships, and can thus offer useful insights into ecosystem functioning. There is now a rapidly-growing interest amongst biologists in applying analysis of environmental DNA to their own research. However, good practices and protocols dealing with environmental DNA are currently widely dispersed across numerous papers, with many of them presenting only preliminary results and using a diversity of methods. In this context, the principal objective of this practical handbook is to provide biologists (both students and researchers) with the scientific background necessary to assist with the understanding and implementation of best practices and analyses based on environmental DNA.
Author: David C. Culver Publisher: Oxford University Press ISBN: 0192552767 Category : Science Languages : en Pages : 336
Book Description
The second edition of this widely cited textbook continues to provide a concise but comprehensive introduction to cave and subterranean biology, describing this fascinating habitat and its biodiversity. It covers a range of biological processes including ecosystem function, evolution and adaptation, community ecology, biogeography, and conservation. The authors draw on a global range of examples and case studies from both caves and non-cave subterranean habitats. One of the barriers to the study of subterranean biology has been the extraordinarily large number of specialized terms used by researchers; the authors explain these terms clearly and minimize the number that they use. This new edition retains the same 10 chapter structure of the original, but the content has been thoroughly revised and updated throughout to reflect the huge increase in publications concerning subterranean biology over the last decade.
Author: Don Graham Publisher: Macmillan + ORM ISBN: 1466867973 Category : Performing Arts Languages : en Pages : 408
Book Description
A larger-than-life narrative of the making of the classic film, marking the rise of America as a superpower, the ascent of Hollywood celebrity, and the flowering of Texas culture as mythology. Featuring James Dean, Rock Hudson, and Elizabeth Taylor, Giant is an epic film of fame and materialism, based around the discovery of oil at Spindletop and the establishment of the King Ranch of south Texas. Isolating his star cast in the wilds of West Texas, director George Stevens brought together a volatile mix of egos, insecurities, sexual proclivities, and talent. Stevens knew he was overwhelmed with Hudson’s promiscuity, Taylor’s high diva-dom, and Dean’s egotistical eccentricity. Yet he coaxed performances out of them that made cinematic history, winning Stevens the Academy Award for Best Director and garnering nine other nominations, including a nomination for Best Actor for James Dean, who died before the film was finished. In this compelling and impeccably researched narrative history of the making of the film, Don Graham chronicles the stories of Stevens, whose trauma in World War II intensified his ambition to make films that would tell the story of America; Edna Ferber, a considerable literary celebrity, who meets her match in the imposing Robert Kleberg, proprietor of the vast King Ranch; and Glenn McCarthy, an American oil tycoon; and Errol Flynn lookalike with a taste for Hollywood. Drawing on archival sources Graham’s Giant is a comprehensive depiction of the film’s production showing readers how reality became fiction and fiction became cinema.
Author: Pierre Taberlet
Author: Pierre Taberlet
Author: Jerney, Jacqueline
Author: Jeremy Barnes
Author: Mary Daheim
Author: 
Author: Pierre Taberlet
Author: David C. Culver
Author: New York (State). Department of Agriculture
Author: 
Author: Don Graham