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Advanced Protocols in Oxidative Stress II

Advanced Protocols in Oxidative Stress II PDF Author: Donald Armstrong
Publisher: Humana Press
ISBN: 9781607614104
Category : Science
Languages : en
Pages : 468

Book Description
Expanding upon the research elucidated by the first volume of this collection, Advanced Protocols in Oxidative Stress II presents thirty additional cutting-edge chapters focusing on novel techniques for detecting ROS/RNS, unique AOX technology and applications, gene expression and biostatistics for evaluating OS-derived experimental data. The international panel of authors also provide animal models and numerous studies concentrating on mitochondria during hypoxic conditions using advanced methods for pO2, peroxynitrate, reactive S-nitrosothiols, lipid peroxides, COX, and the mitochondrial membrane potential. Due to the dynamic nature of this topic, this book is the second of several volumes of Advanced Protocols in Oxidative Stress, all included in the highly successful Methods in Molecular BiologyTM series. As part of the series, the chapters of this volume present brief introductions to the respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting to ensure easy replication of the technology involved. Authoritative and convenient, Advanced Protocols in Oxidative Stress II is an ideal desk reference for scientists wishing to further the research in this exciting, unique, and vital field of study.

Advanced Protocols in Oxidative Stress II

Advanced Protocols in Oxidative Stress II PDF Author: Donald Armstrong
Publisher: Humana Press
ISBN: 9781607614104
Category : Science
Languages : en
Pages : 468

Book Description
Expanding upon the research elucidated by the first volume of this collection, Advanced Protocols in Oxidative Stress II presents thirty additional cutting-edge chapters focusing on novel techniques for detecting ROS/RNS, unique AOX technology and applications, gene expression and biostatistics for evaluating OS-derived experimental data. The international panel of authors also provide animal models and numerous studies concentrating on mitochondria during hypoxic conditions using advanced methods for pO2, peroxynitrate, reactive S-nitrosothiols, lipid peroxides, COX, and the mitochondrial membrane potential. Due to the dynamic nature of this topic, this book is the second of several volumes of Advanced Protocols in Oxidative Stress, all included in the highly successful Methods in Molecular BiologyTM series. As part of the series, the chapters of this volume present brief introductions to the respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting to ensure easy replication of the technology involved. Authoritative and convenient, Advanced Protocols in Oxidative Stress II is an ideal desk reference for scientists wishing to further the research in this exciting, unique, and vital field of study.

Oxidative Stress and Antioxidant Protection

Oxidative Stress and Antioxidant Protection PDF Author: Donald Armstrong
Publisher: John Wiley & Sons
ISBN: 111883240X
Category : Science
Languages : en
Pages : 742

Book Description
Oxidative Stress and Antioxidant Protection: The Science of Free Radical Biology and Disease Oxidative Stress and Antioxidant Protection begins with a historical perspective of pioneers in oxidative stress with an introductory section that explains the basic principles related to oxidative stress in biochemistry and molecular biology, demonstrating both pathways and biomarkers. This section also covers diagnostic imaging and differential diagnostics. The following section covers psychological, physiologic, pharmacologic and pathologic correlates. This section addresses inheritance, gender, nutrition, obesity, family history, behavior modification, natural herbal-botanical products, and supplementation in the treatment of disease. Clinical trials are also summarized for major medical disorders and efficacy of treatment, with particular focus on inflammation, immune response, recycling, disease progression, outcomes and interventions. Each of the chapters describes what biomarker(s) and physiological functions may be relevant to a concept of specific disease and potential alternative therapy. The chapters cover medical terminology, developmental change, effects of aging, senescence, lifespan, and wound healing, and also illustrates cross-over exposure to other fields. The final chapter covers how and when to interpret appropriate data used in entry level biostatistics and epidemiology. Authored and edited by leaders in the field, Oxidative Stress and Antioxidant Protection will be an invaluable resource for students and researchers studying cell biology, molecular biology, and biochemistry, as well professionals in various health science fields.

Advanced Protocols in Oxidative Stress II

Advanced Protocols in Oxidative Stress II PDF Author: Donald Armstrong
Publisher: Humana
ISBN: 9781493957033
Category : Science
Languages : en
Pages : 444

Book Description
Expanding upon the research elucidated by the first volume of this collection, Advanced Protocols in Oxidative Stress II presents thirty additional cutting-edge chapters focusing on novel techniques for detecting ROS/RNS, unique AOX technology and applications, gene expression and biostatistics for evaluating OS-derived experimental data. The international panel of authors also provide animal models and numerous studies concentrating on mitochondria during hypoxic conditions using advanced methods for pO2, peroxynitrate, reactive S-nitrosothiols, lipid peroxides, COX, and the mitochondrial membrane potential. Due to the dynamic nature of this topic, this book is the second of several volumes of Advanced Protocols in Oxidative Stress, all included in the highly successful Methods in Molecular BiologyTM series. As part of the series, the chapters of this volume present brief introductions to the respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting to ensure easy replication of the technology involved. Authoritative and convenient, Advanced Protocols in Oxidative Stress II is an ideal desk reference for scientists wishing to further the research in this exciting, unique, and vital field of study.

Advanced Protocols in Oxidative Stress I

Advanced Protocols in Oxidative Stress I PDF Author: Donald Armstrong
Publisher: Humana Press
ISBN: 9781603279277
Category : Science
Languages : en
Pages : 430

Book Description
Protocols books specializing in measuring free radical and antioxidant biomarkers began to be published in 1998. Many of these methods are currently finding use in diagnostic medicine. Advanced Protocols in Oxidative Stress I covers the field of oxidative stress with state-of-the-art technology to utilize in research, contributed by an international panel of experts renowned for developing new procedures and methods. Included are sections on reactive oxygen and nitrogen species techniques, antioxidant technology and application, methods for analyzing gene expression, the exciting new area of oxidative stress and stem cell differentiation and specific biostatistical evaluation of biomarkers. This volume presents the current high-tech methodologies and provides a perspective on the diversity of applications in the ever-emerging field of free radical reactions and antioxidants. Due to the dynamic nature of this topic, this book will be the first of several volumes of Advanced Protocols in Oxidative Stress, all part of the highly successful Methods in Molecular BiologyTM series. As part of the series, the chapters include a brief introduction to the material, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and ensuring replication of technology. Cutting-edge and convenient, Advanced Protocols in Oxidative Stress I is an ideal desk reference for scientists wishing to further this research in this exciting, unique and vital field of study.

Fluorescent Tools for Imaging Oxidative Stress in Biology

Fluorescent Tools for Imaging Oxidative Stress in Biology PDF Author: Amandeep Kaur
Publisher: Springer
ISBN: 3319734059
Category : Science
Languages : en
Pages : 265

Book Description
This thesis advances the long-standing challenge of measuring oxidative stress and deciphering its underlying mechanisms, and also outlines the advantages and limitations of existing design strategies. It presents a range of approaches for the chemical synthesis of fluorescent probes that detect reversible changes in cellular oxidative stress. The ability to visualise cellular processes in real-time is crucial to understanding disease development and streamline treatment, and this can be achieved using fluorescent tools that can sense reversible disturbances in cellular environments during pathogenesis. The perturbations in cellular redox state are of particular current interest in medical research, since oxidative stress is implicated in the pathogenesis of a number of diseases. The book investigates different strategies used to achieve ratiometric fluorescence output of the reversible redox probes, which nullify concentration effects associated with intensity-based probes. It also describes suitable approaches to target these probes to specific cellular organelles, thereby enabling medical researchers to visualise sub-cellular oxidative stress levels, and addressing the typically poor uptake of chemical tools into biological studies. In total it reports on four new probes that are now being used by over twenty research groups around the globe, and two of which have been commercialised. The final chapters of this thesis demonstrate successful applications of the sensors in a variety of biological systems ranging from prokaryotes to mammalian cells and whole organisms. The results described clearly indicate the immense value of collaborative, cross-disciplinary research.

Measuring Oxidants and Oxidative Stress in Biological Systems

Measuring Oxidants and Oxidative Stress in Biological Systems PDF Author: Lawrence J. Berliner
Publisher: Springer Nature
ISBN: 303047318X
Category : Science
Languages : en
Pages : 237

Book Description
This book describes the methods of analysis and determination of oxidants and oxidative stress in biological systems. Reviews and protocols on select methods of analysis of ROS, RNS, oxygen, redox status, and oxidative stress in biological systems are described in detail. It is an essential resource for both novices and experts in the field of oxidant and oxidative stress biology.

Advances in Plant Ecophysiology Techniques

Advances in Plant Ecophysiology Techniques PDF Author: Adela M. Sánchez-Moreiras
Publisher: Springer
ISBN: 3319932330
Category : Science
Languages : en
Pages : 491

Book Description
This handbook covers the most commonly used techniques for measuring plant response to biotic and abiotic stressing factors, including: in vitro and in vivo bioassays; the study of root morphology, photosynthesis (pigment content, net photosynthesis, respiration, fluorescence and thermoluminiscence) and water status; thermal imaging; the measurement of oxidative stress markers; flow cytometry for measuring cell cycle and other physiological parameters; the use of microscope techniques for studying plant microtubules; programmed-cell-death; last-generation techniques (metabolomics, proteomics, SAR/QSAR); hybridization methods; isotope techniques for plant and soil studies; and the measurement of detoxification pathways, volatiles, soil microorganisms, and computational biology.

Advances in Food Science and Nutrition, Volume 2

Advances in Food Science and Nutrition, Volume 2 PDF Author: Visakh P. M.
Publisher: John Wiley & Sons
ISBN: 1118865634
Category : Technology & Engineering
Languages : en
Pages : 566

Book Description
This important book comprehensively reviews research on new developments in all areas of food chemistry/science and nutrition Advances in Food Science and Nutrition covers topics such as food safety objectives, risk assessment, quality assurance and control, good manufacturing practices, food processing systems, design and control, and rapid methods of analysis and detection, as well as sensor technology, environmental control, and safety. The thirteen chapters are written by prominent researchers from industry, academia, and government/private research laboratories around the world. The book details many of the recent technical research accomplishments in the areas of food science, including: Potato production, composition, and starch processing Milk and different types of milk products Processing and preservation of meat, poultry, and seafood Food ingredients including additives and natural plant-based ingredients Fruits and fruit processing Antioxidant activity of phytochemicals and their method of analysis The effect of food processing on bioactive compounds Food safety regulations including foodborne pathogens, probiotics, genetically modified foods, and bioavailability of nutrients Trends in sensory characterization of food products Ultrasound applications in food technology Transformations of food flavor including aroma compounds and chemical reactions that influence flavor Storage technologies for fresh fruits

High Performance Logic And Circuits For High-speed Electronic Systems

High Performance Logic And Circuits For High-speed Electronic Systems PDF Author: Faquir C Jain
Publisher: World Scientific
ISBN: 981120845X
Category : Technology & Engineering
Languages : en
Pages : 191

Book Description
In this volume, we have put together papers spanning a broad range — from the area of modeling of strain and misfit dislocation densities, microwave absorption characteristics of nanocomposites, to X-ray diffraction studies.Specific topics in this volume include:In summary, papers selected in this volume cover various aspects of high performance logic and circuits for high-speed electronic systems.

Progress in Volatile Organic Compounds Research

Progress in Volatile Organic Compounds Research PDF Author: Igor Jerković
Publisher: MDPI
ISBN: 3039288717
Category : Science
Languages : en
Pages : 228

Book Description
Volatile organic compounds (VOCs) have been intensively investigated in the last few decades. Their origins differ: plant secondary metabolites, food/beverages aromas, fungal/bacterial volatiles, and others. VOCs typically occur as complex mixtures of compounds (e.g., monoterpenes, sesquiterpenes, norisoprenoids, aliphatic/aromatic compounds, sulfur containing compounds, and others). They form through different biochemical pathways and can be modified or created during drying or maturation, thermal treatment, and others. Different conventional or modern methods of VOCs isolation, followed by the analysis with chromatographic and spectroscopic techniques, usually provide different chemical profiles and have been under constant modification and upgrading. The ecological interactions are mediated by VOCs (inter- and intra-organismic communication) and they can act as pheromones, attractants, or alleochemicals. Among them, chemical biomarkers of botanical origin or chemotaxonomic markers may be found. Many VOCs possess different biological activities, such as antioxidant, antimicrobial, antiviral, anticancer, and other activities. VOCs research from different sources is required to report their distribution and chemical profiles, and to discover new compounds. This Special Issue aims to attract up-to-date contributions on all aspects of VOCs chemistry, from challenges in their isolation to analysis, and on unlocking their biological activities or other useful properties