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Author: Alfred Pühler Publisher: Springer Science & Business Media ISBN: 3642693059 Category : Science Languages : en Pages : 353
Book Description
The development of powerful new techniques and refmements of tech niques in molecular genetics in recent years, and the surge in interest in biotechnology based on genetic methods, have heralded a new golden age in molecular genetics, and stimulated in diverse disciplines much interest in the technologies themselves and their potential uses in basic and applied biomedical sciences. Although some excellent specialist laboratory manuals (especially the Cold Spring Harbor Laboratory manuals by I. H. Miller; R. W. Davies et al. ; and T. Maniatis et al. ) on certain chapters of molecular genetics exist, no general text that covers a broad spectrum of the sub ject has thus far been published. The purpose of this manual is to pre sent most, though of necessity not all of the important methods of molecular genetics, in a series of simple experiments, many of which can be readily accomplished by the microbiologist, biochemist or biotechnologist that has had only limited exposure to genetics. The remainder of the experiments require either greater familiarity with the subject, or guidance by someone with such experience. The book should, therefore, not only enable individuals to acquire new proce dures for ongoing projects, but also serve as a basis for the teaching of molecular genetic techniques in formal predoctoral and postdoctoral laboratory courses.
Author: Richard M. Twyman Publisher: Taylor & Francis ISBN: 9781859961414 Category : Science Languages : en Pages : 499
Book Description
Advanced Molecular Biology - A Concise Reference provides in-depth coverage of 30 essential topics in molecular biology with particular focus on genetic information and its expression. The book emphasizes unifying principles and mechanisms, with comprehensive use of tables and boxes to summarize experimental data, gene and protein functions. Advanced Molecular Biology - A Concise Reference is written for upper level undergraduates, postgraduates and academics with an interest in molecular biology who need a convenient entry into the field.
Author: Khalid Z. Masoodi Publisher: Academic Press ISBN: 012824450X Category : Science Languages : en Pages : 220
Book Description
Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. - Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology - Features clear, step-by-step instruction for applying the techniques covered - Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment
Author: Alfred Pühler Publisher: Springer Science & Business Media ISBN: 3642693059 Category : Science Languages : en Pages : 353
Book Description
The development of powerful new techniques and refmements of tech niques in molecular genetics in recent years, and the surge in interest in biotechnology based on genetic methods, have heralded a new golden age in molecular genetics, and stimulated in diverse disciplines much interest in the technologies themselves and their potential uses in basic and applied biomedical sciences. Although some excellent specialist laboratory manuals (especially the Cold Spring Harbor Laboratory manuals by I. H. Miller; R. W. Davies et al. ; and T. Maniatis et al. ) on certain chapters of molecular genetics exist, no general text that covers a broad spectrum of the sub ject has thus far been published. The purpose of this manual is to pre sent most, though of necessity not all of the important methods of molecular genetics, in a series of simple experiments, many of which can be readily accomplished by the microbiologist, biochemist or biotechnologist that has had only limited exposure to genetics. The remainder of the experiments require either greater familiarity with the subject, or guidance by someone with such experience. The book should, therefore, not only enable individuals to acquire new proce dures for ongoing projects, but also serve as a basis for the teaching of molecular genetic techniques in formal predoctoral and postdoctoral laboratory courses.
Author: Heather B. Miller Publisher: Academic Press ISBN: 0123855454 Category : Science Languages : en Pages : 227
Book Description
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Author: Richard Twyman Publisher: Garland Science ISBN: 1135324468 Category : Science Languages : en Pages : 763
Book Description
Advanced Molecular Biology emphasises the unifying principles and mechanisms of molecular biology, with frequent use of tables and boxes to summarise experimental data and gene and protein functions. Extensive cross-referencing between chapters is used to reinforce and broaden the understanding of core concepts. This is the ideal source of comprehensive, authoritative and up-to-date information for all those whose work is in the field of molecular biology. This book emphasises the unifying principles and mechanisms of molecular biology, with frequent use of tables and boxes to summarise experimental data and gene and protein functions.
Author: Richard M. Twyman Publisher: Springer ISBN: Category : Medical Languages : en Pages : 522
Book Description
A well organized reference that provides clear and concise explanations of the terminology used in molecular biology and genetics. It covers key areas such as the cell cycle, organelles and plasmids, replication and recombinational, transcription, gene expression and regulation, and PCR. In addition, DNA, RNA and chromosomes are covered, including their structure, function, modification, mutation and repair. 80 illus.
Author: Richard M. Twyman Publisher: Springer ISBN: 9789813083813 Category : Molecular biology Languages : en Pages : 499
Book Description
Advanced Molecular Biology - A Concise Reference provides in-depth coverage of 30 essential topics in molecular biology with particular focus on genetic information and its expression. The book emphasizes unifying principles and mechanisms, with comprehensive use of tables and boxes to summarize experimental data, gene and protein functions. Advanced Molecular Biology - A Concise Reference is written for upper level undergraduates, postgraduates and academics with an interest in molecular biology who need a convenient entry into the field.
Author: John Wilson Publisher: Garland Science ISBN: 1317497279 Category : Science Languages : en Pages : 984
Book Description
The Problems Book helps students appreciate the ways in which experiments and simple calculations can lead to an understanding of how cells work by introducing the experimental foundation of cell and molecular biology. Each chapter reviews key terms, tests for understanding basic concepts, and poses research-based problems. The Problems Book has be
Author: Swati Agarwal Publisher: I K International Pvt Ltd ISBN: 9385909967 Category : Medical Languages : en Pages : 166
Book Description
This book provides detailed information on various instruments, techniques and experiment protocols of biochemistry and molecular biology. It deals with basic as well as advanced information and in-depth methodology in simple language to help students and professionals to perform experiments with ease. This book not only clears the practical concepts of Biochemistry and Molecular Biology at undergraduate and post-graduation levels, but also helps to pass the Ph.D. course work exam conducted by various universities. This book will develop research aptitude to clear the NET examination. This manual gives a comprehensive idea about the various instruments, their working, troubleshooting and their applications. It provides a wide spectrum of 14 chapters covering basic as well as advanced techniques and instrumentation, viz., Gas Chromatography (GC), Mass Spectrometry (MS), Scanning Electron Microscope (SEM), X-Ray Diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR) with detailed protocols. Most of the experiments can be easily performed in the laboratory having basic facilities. Historical background, experiment nature, its principle, step-by-step procedure with diagrammatic representation and important precautions are given in the beginning of each experiment.
Author: Alfred Pühler
Author: Richard M. Twyman
Author: Khalid Z. Masoodi
Author: Alfred Pühler
Author: Heather B. Miller
Author: Richard Twyman
Author: Richard M. Twyman
Author: Richard M. Twyman
Author: 
Author: John Wilson
Author: Swati Agarwal