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Author: Randall Mark Stoltenberg Publisher: Stanford University ISBN: Category : Languages : en Pages : 249
Book Description
Directed assembly of single molecules is a central theme in nanotechnology. This body of work was inspired by a specific challenge involving ordered deposition of single DNAs on surfaces for massively parallel single molecule DNA sequencing via fluorescence microscopy. A potential 10-fold gain in data density is possible if single molecules can be forced into a regular array rather than randomly deposited. The dimensions of such an array are difficult to achieve with conventional lithography techniques. On one end, molecules must be separated by sufficient distance so their optical signatures do not overlap. This distance is on the order of hundreds of nanometers. On the other end, the attachment points for the molecules must have molecular dimensions. Bridging these two length scales is a formidable task. The ability to place nanometer scale objects with nanometer precision can been achieved through atomic force microscopy, scanning tunneling microscopy, optical tweezers, and ebeam lithography. All of these techniques, however, are serial in nature and hence do not serve the intended gain in data density. Another approach toward directed patterning of single molecules is through self-assembly. In this work, self-assembly of block copolymers is explored as a means to addressing the molecular and optical resolution length scales simultaneously. First, the challenge of molecular patterning for single molecule fluorescence microscopy is explored theoretically and the limits of this approach are defined. Block copolymers are introduced as a possible solution to generating the correct surface patterns for improved data density, and experimental results are compared to theoretical predictions. Second, the surface chemistry of these arrays is characterized, and I will show they can be selectively functionalized in preparation for directed assembly of DNAs. Third, these arrays are integrated into single molecule fluorescence imaging experiments to determine their potential for improved data density. What emerges from this work is not only a viable platform for increased single molecule fluorescence data density, but also a deeper understanding of the requirements for directed self-assembly of single molecules.
Author: Randall Mark Stoltenberg Publisher: Stanford University ISBN: Category : Languages : en Pages : 249
Book Description
Directed assembly of single molecules is a central theme in nanotechnology. This body of work was inspired by a specific challenge involving ordered deposition of single DNAs on surfaces for massively parallel single molecule DNA sequencing via fluorescence microscopy. A potential 10-fold gain in data density is possible if single molecules can be forced into a regular array rather than randomly deposited. The dimensions of such an array are difficult to achieve with conventional lithography techniques. On one end, molecules must be separated by sufficient distance so their optical signatures do not overlap. This distance is on the order of hundreds of nanometers. On the other end, the attachment points for the molecules must have molecular dimensions. Bridging these two length scales is a formidable task. The ability to place nanometer scale objects with nanometer precision can been achieved through atomic force microscopy, scanning tunneling microscopy, optical tweezers, and ebeam lithography. All of these techniques, however, are serial in nature and hence do not serve the intended gain in data density. Another approach toward directed patterning of single molecules is through self-assembly. In this work, self-assembly of block copolymers is explored as a means to addressing the molecular and optical resolution length scales simultaneously. First, the challenge of molecular patterning for single molecule fluorescence microscopy is explored theoretically and the limits of this approach are defined. Block copolymers are introduced as a possible solution to generating the correct surface patterns for improved data density, and experimental results are compared to theoretical predictions. Second, the surface chemistry of these arrays is characterized, and I will show they can be selectively functionalized in preparation for directed assembly of DNAs. Third, these arrays are integrated into single molecule fluorescence imaging experiments to determine their potential for improved data density. What emerges from this work is not only a viable platform for increased single molecule fluorescence data density, but also a deeper understanding of the requirements for directed self-assembly of single molecules.
Author: Publisher: Academic Press ISBN: 008095927X Category : Science Languages : en Pages : 559
Book Description
Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part A: Fluorescence Based Approaches captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. - Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools - Methods included are useful to both established researchers and newcomers to the field - Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines
Author: Alberto Fernandez-Nieves Publisher: John Wiley & Sons ISBN: 111806562X Category : Technology & Engineering Languages : en Pages : 444
Book Description
This book presents a compilation of self-contained chapters covering a wide range of topics within the broad field of soft condensed matter. Each chapter starts with basic definitions to bring the reader up-to-date on the topic at hand, describing how to use fluid flows to generate soft materials of high value either for applications or for basic research. Coverage includes topics related to colloidal suspensions and soft materials and how they differ in behavior, along with a roadmap for researchers on how to use soft materials to study relevant physics questions related to geometrical frustration.
Author: Bo Cui Publisher: BoD – Books on Demand ISBN: 953307602X Category : Science Languages : en Pages : 630
Book Description
Nanotechnology has experienced a rapid growth in the past decade, largely owing to the rapid advances in nanofabrication techniques employed to fabricate nano-devices. Nanofabrication can be divided into two categories: "bottom up" approach using chemical synthesis or self assembly, and "top down" approach using nanolithography, thin film deposition and etching techniques. Both topics are covered, though with a focus on the second category. This book contains twenty nine chapters and aims to provide the fundamentals and recent advances of nanofabrication techniques, as well as its device applications. Most chapters focus on in-depth studies of a particular research field, and are thus targeted for researchers, though some chapters focus on the basics of lithographic techniques accessible for upper year undergraduate students. Divided into five parts, this book covers electron beam, focused ion beam, nanoimprint, deep and extreme UV, X-ray, scanning probe, interference, two-photon, and nanosphere lithography.
Author: Alessandra Cambi Publisher: CRC Press ISBN: 1482209896 Category : Medical Languages : en Pages : 514
Book Description
Cell Membrane Nanodomains: From Biochemistry to Nanoscopy describes recent advances in our understanding of membrane organization, with a particular focus on the cutting-edge imaging techniques that are making these new discoveries possible. With contributions from pioneers in the field, the book explores areas where the application of these novel techniques reveals new concepts in biology. It assembles a collection of works where the integration of membrane biology and microscopy emphasizes the interdisciplinary nature of this exciting field. Beginning with a broad description of membrane organization, including seminal work on lipid partitioning in model systems and the roles of proteins in membrane organization, the book examines how lipids and membrane compartmentalization can regulate protein function and signal transduction. It then focuses on recent advances in imaging techniques and tools that foster further advances in our understanding of signaling nanoplatforms. The coverage includes several diffraction-limited imaging techniques that allow for measurements of protein distribution/clustering and membrane curvature in living cells, new fluorescent proteins, novel Laurdan analyses, and the toolbox of labeling possibilities with organic dyes. Since superresolution optical techniques have been crucial to advancing our understanding of cellular structure and protein behavior, the book concludes with a discussion of technologies that are enabling the visualization of lipids, proteins, and other molecular components at unprecedented spatiotemporal resolution. It also explains the ins and outs of the rapidly developing high- or superresolution microscopy field, including new methods and data analysis tools that exclusively pertain to these techniques. This integration of membrane biology and advanced imaging techniques emphasizes the interdisciplinary nature of this exciting field. The array of contributions from leading world experts makes this book a valuable tool for the visualization of signaling nanoplatforms by means of cutting-edge optical microscopy tools.
Author: Publisher: Elsevier ISBN: 0128164646 Category : Science Languages : en Pages : 404
Book Description
Spectroscopy and Dynamics of Single Molecules: Methods and Applications reviews the most recent developments in spectroscopic methods and applications. Spectroscopic techniques are the chief experimental methods for testing theoretical models and research in this area plays an important role in stimulating new theoretical developments in physical chemistry. This book provides an authoritative insight into the latest advances in the field, highlighting new techniques, current applications, and potential future developments An ideal reference for chemists and physicists alike, Spectroscopy and Dynamics of Single Molecules: Methods and Applications is a useful guide for all those working in the research, design, or application of spectroscopic tools and techniques across a wide range of fields. - Includes the latest research on ultrafast vibrational and electronic dynamics, nonlinear spectroscopies, and single-molecule methods - Makes the content accessible to researchers in chemistry, biophysics, and chemical physics through a rigorous multi-disciplinary approach - Provides content edited by a world-renowned chemist with more than 30 years of experience in research and instruction
Author: Alexei A. Maradudin Publisher: Cambridge University Press ISBN: 1139497871 Category : Science Languages : en Pages : 459
Book Description
Optical metamaterials are an exciting new field in optical science. A rapidly developing class of these metamaterials are those that allow the manipulation of volume and surface electromagnetic waves in desirable ways by suitably structuring the surfaces they interact with. They have applications in a variety of fields, such as materials science, photovoltaic technology, imaging and lensing, beam shaping and lasing. Describing techniques and applications, this book is ideal for researchers and professionals working in metamaterials and plasmonics, as well as those just entering this exciting new field. It surveys different types of structured surfaces, their design and fabrication, their unusual optical properties, recent experimental observations and their applications. Each chapter is written by an expert in that area, giving the reader an up-to-date overview of the subject. Both the experimental and theoretical aspects of each topic are presented.
Author: Philip Tinnefeld Publisher: Springer ISBN: 3662455471 Category : Science Languages : en Pages : 340
Book Description
This book describes developments in the field of super-resolution fluorescence microscopy or nanoscopy. In 11 chapters, distinguished scientists and leaders in their respective fields describe different nanoscopy approaches, various labeling technologies, and concrete applications. The topics covered include the principles and applications of the most popular nanoscopy techniques STED and (f)PALM/STORM, along with advances brought about by fluorescent proteins and organic dyes optimized for fluorescence nanoscopy. Furthermore, the photophysics of fluorescent labels is addressed, specifically for improving their photoswitching capabilities. Important applications are also discussed, such as the tracking and counting of molecules to determine acting forces in cells, and quantitative cellular imaging, respectively, as well as the mapping of chemical reaction centers at the nano-scale. The 2014 Chemistry Nobel Prize® was awarded for the ground-breaking developments of super-resolved fluorescence microscopy. In this book, which was co-edited by one of the prize winners, readers will find the most recent developments in this field.
Author: Randall Mark Stoltenberg
Author: Randall Mark Stoltenberg
Author: 
Author: Alberto Fernandez-Nieves
Author: Bo Cui
Author: Alessandra Cambi
Author: 
Author: 
Author: Alexei A. Maradudin
Author: Moran Rubinstein
Author: Philip Tinnefeld