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Author: Michael J. Dykstra Publisher: Springer Science & Business Media ISBN: 9780306444494 Category : Medical Languages : en Pages : 280
Book Description
This easy-to-follow manual describes tested procedures used to prepare biological samples for scanning and transmission electron microscopy, as well as methods for cytochemistry, immunocytochemistry, and scientific photography. The work is structured to clearly define testing objectives, necessary materials, procedural steps, and expected results; a list of references and trouble shooting techniques round out the text.
Author: Michael J. Dykstra Publisher: Springer Science & Business Media ISBN: 9780306444494 Category : Medical Languages : en Pages : 280
Book Description
This easy-to-follow manual describes tested procedures used to prepare biological samples for scanning and transmission electron microscopy, as well as methods for cytochemistry, immunocytochemistry, and scientific photography. The work is structured to clearly define testing objectives, necessary materials, procedural steps, and expected results; a list of references and trouble shooting techniques round out the text.
Author: Michael J. Dykstra Publisher: Springer Science & Business Media ISBN: 9780306477492 Category : Science Languages : en Pages : 558
Book Description
Electron microscopy is frequently portrayed as a discipline that stands alone, separated from molecular biology, light microscopy, physiology, and biochemistry, among other disciplines. It is also presented as a technically demanding discipline operating largely in the sphere of "black boxes" and governed by many absolute laws of procedure. At the introductory level, this portrayal does the discipline and the student a disservice. The instrumentation we use is complex, but ultimately understandable and, more importantly, repairable. The procedures we employ for preparing tissues and cells are not totally understood, but enough information is available to allow investigators to make reasonable choices concerning the best techniques to apply to their parti cular problems. There are countless specialized techniques in the field of electron and light microscopy that require the acquisition of specialized knowledge, particularly for interpretation of results (electron tomography and energy dispersive spectroscopy immediately come to mind), but most laboratories possessing the equipment to effect these approaches have specialists to help the casual user. The advent of computer operated electron microscopes has also broadened access to these instruments, allowing users with little technical knowledge about electron microscope design to quickly become operators. This has been a welcome advance, because earlier instru ments required a level of knowledge about electron optics and vacuum systems to produce optimal photographs and to avoid "crashing" the instruments that typically made it difficult for beginners.
Author: Elaine Evelyn Hunter Publisher: Cambridge University Press ISBN: 9780521385398 Category : Medical Languages : en Pages : 212
Book Description
For this new edition, the chapters on photography and the electron microscope have been completely rewritten and two new chapters have been added--on immuno electron microscopy using colloidal gold and on useful specialized techniques.
Author: M. J. Reigosa Roger Publisher: Springer Science & Business Media ISBN: 0306480573 Category : Science Languages : en Pages : 450
Book Description
The Handbook of Plant Ecophysiology Techniques you have now in your hands is the result of several combined events and efforts. The birth of this handbook can be traced as far as 1997, when our Plant Ecophysiology lab at the University of Vigo hosted a practical course on Plant Ecophysiology Techniques. That course showed us how much useful a handbook presenting a bunch of techniques would be for the scientists beginning to work on Plant Ecophysiology. In fact, we wrote a short handbook explaining the basics of the techniques taught in that 1997 course: Flow cytometry to measure ploidy levels, Use of a Steady-State porometer to measure transpiration, In vivo measure of fluorescence, HPLC analysis of low molecular weight phenolics, Spectrophotometric determinations of free proline and soluble proteins, TLC polyamines contents measures, Isoenzymatic electrophoresis, Use of IRGA and oxygen electrode. That modest handbook, written in Spanish, was very helpful, both for the people who attended the course and for other who have used it for beginning to work in Plant Ecophysiology. The present Handbook is much more ambitious, and it includes more techniques. But we have also had in mind the young scientists beginning to work on Plant Ecophysiology. In 1999 François Pellissier leaded a proposal presented to the European Commission in the Fifth Framework Program in the High Level * Scientific Conferences, including three EuroLab Courses about lab and field techniques useful to improve allelopathic research.
Author: Diane Schmidt Publisher: CRC Press ISBN: 0824741714 Category : Language Arts & Disciplines Languages : en Pages : 484
Book Description
"Provides an in-depth review of current print and electronic tools for research in numerous disciplines of biology, including dictionaries and encyclopedias, method guides, handbooks, on-line directories, and periodicals. Directs readers to an associated Web page that maintains the URLs and annotations of all major Inernet resources discussed in th
Author: Patrick Echlin Publisher: Springer Science & Business Media ISBN: 0387857311 Category : Technology & Engineering Languages : en Pages : 329
Book Description
Scanning electr on microscopy (SEM) and x-ray microanalysis can produce magnified images and in situ chemical information from virtually any type of specimen. The two instruments generally operate in a high vacuum and a very dry environment in order to produce the high energy beam of electrons needed for imaging and analysis. With a few notable exceptions, most specimens destined for study in the SEM are poor conductors and composed of beam sensitive light elements containing variable amounts of water. In the SEM, the imaging system depends on the specimen being sufficiently electrically conductive to ensure that the bulk of the incoming electrons go to ground. The formation of the image depends on collecting the different signals that are scattered as a consequence of the high energy beam interacting with the sample. Backscattered electrons and secondary electrons are generated within the primary beam-sample interactive volume and are the two principal signals used to form images. The backscattered electron coefficient ( ? ) increases with increasing atomic number of the specimen, whereas the secondary electron coefficient ( ? ) is relatively insensitive to atomic number. This fundamental diff- ence in the two signals can have an important effect on the way samples may need to be prepared. The analytical system depends on collecting the x-ray photons that are generated within the sample as a consequence of interaction with the same high energy beam of primary electrons used to produce images.
Author: Ronald R. Watson Publisher: CRC Press ISBN: 1000151794 Category : Medical Languages : en Pages : 420
Book Description
Written by the international community's leading experts, Trace Elements in Laboratory Rodents describes the best and most current methods to provide deficient or supplemental trace elements to laboratory animals, as well as how to assay them. The experts warn of the common pitfalls and hidden problems in nutritional testing and how to avoid them. This how-to approach focuses on the technical details that make good, reliable studies. Common as well as rare or recently recognized minerals are described relating to both dietary supplementation and measurement in tissues. If you are a researcher, professor, or student working in nutrition, food science, biochemistry, or veterinary medicine, you can't afford to be without this excellent hands-on methods manual!
Author: Marziale Milani Publisher: Springer Nature ISBN: 3031269497 Category : Medical Languages : en Pages : 242
Book Description
This interdisciplinary work deals with the bacterial degradation of organic and inorganic materials such as prosthetic devices and the consequent production of non-engineered nanoparticles (NPs). Focus is put on the interaction of these, often toxic, NPs with the environment, the microorganisms and the host human body. Electron Microscopy is the method of choice to investigate bacterial colonization and degradation of plastic polymers. Hence one section of the book is fully dedicated to the most recent and interesting microscopy technologies in microbiology and soft matters. The final chapter of the book on the complex and multivariate relationships between a microscopist and electron microscopy images is dedicated to Lyubov Vasilievna Didenko (1958 – 2015), a passionate researcher who contributed substantially to the field of Electron Microscopy research and its applications in studying bacterial-polymer interactions. The book addresses researchers and advanced students working in general and clinical microbiology, nanobiology, materials sciences and image analysis fields.
Author: Michael J. Dykstra
Author: Michael J. Dykstra
Author: Michael J. Dykstra
Author: Elaine Evelyn Hunter
Author: M. J. Reigosa Roger
Author: Diane Schmidt
Author: Patrick Echlin
Author: Allen Kent
Author: Ronald R. Watson
Author: Marziale Milani
Author: Anupam Rajak